| DKS Groundworks Ltd | Construction | 2025 |
| Dlns Holdings Ltd | Hospitality | 2026 |
| DMA Fire Protection Ltd | Construction | 2019 |
| DMD Elite Deals Ltd | Real estate | 2023 |
| DMD Home Improvements Limited | Construction | 2016 |
| DMD Real Estate Group Limited | Real estate | 2023 |
| Dna-Uk Limited | Professional & scientific | 2025 |
| Docherty Engineering & Construction Limited | Wholesale & retail | 2000 |
| Doherty Services Group Limited | Professional & scientific | 2021 |
| Dolce Delicieux Ltd | Hospitality | 2023 |
| Domi Steelfixing Limited | Construction | 2017 |
| Dominatus Ltd | Wholesale & retail | 2024 |
| Don'T ASK Meow Ltd | Wholesale & retail | 2025 |
| Donjamat Ltd | Households | 2019 |
| Doran Hair Limited | Other services | 2011 |
| Dorys Little Scents Limited | Wholesale & retail | 2024 |
| Dory’S Little Socials Limited | Professional & scientific | 2024 |
| Douro Valley Limited | Hospitality | 2014 |
| DR Aslam Medical Services Limited | Health & social care | 2015 |
| Draystons Motorcycles Limited | Wholesale & retail | 2020 |
| DRC Properties Essex Limited | Real estate | 2019 |
| Dream Yard Ltd | Hospitality | 2019 |
| Driving Pride CIC | Other services | 2022 |
| Dsho's Grill Limited | Hospitality | 2016 |
| DSI Construction Limited | Construction | 2016 |
| Ductwork Hygiene Solutions Ltd | Administrative & support | 2019 |
| DUO and Skin Limited | Health & social care | 2024 |
| DUO Clinic Ltd | Health & social care | 2021 |
| Duration (U.P.V.C.) Limited | Professional & scientific | 1989 |
| Duration Windows Limited | Professional & scientific | 1989 |
| E & E Car Sales Ltd | Wholesale & retail | 2017 |
| Eagle Scaffolding Contracts Limited | Construction | 1999 |
| Earl & Manley Property Maintenance Limited | Construction | 2012 |
| Early Years Contracting Limited | Professional & scientific | 2021 |
| Easmi Limited | Health & social care | 2022 |
| East Coast Developments Limited | Construction | 1986 |
| East Coast Farm Contracts Ltd | Agriculture & fishing | 2001 |
| East Street ML Ltd | Real estate | 2025 |
| Eastside Properties Ltd | Real estate | 2024 |
| EAT Well Live Well Ltd | Wholesale & retail | 2025 |
| Eazl C.I.C. | Administrative & support | 2012 |
| Ebi's International Trading Co. Ltd | Wholesale & retail | 2018 |
| EBJ Consulting Limited | Professional & scientific | 2026 |
| Ebrit Services Limited | Other services | 2011 |
| ECG Kingsford Drive Limited | Health & social care | 2025 |
| Echo Romeo LLP | | 2013 |
| Eden Bookkeeping Solutions Ltd | Professional & scientific | 2021 |
| Eden Cocktails Ltd | Hospitality | 2023 |
| Eden Financial Advisers Ltd | Financial services | 2019 |
| EGM Plumbing and Heating Ltd | Construction | 2026 |
| Eholstah Ltd | Real estate | 2026 |
| Elecmac Electrical Limited | Construction | 2009 |
| Electecpro Limited | Professional & scientific | 2025 |
| Electrigal Ltd | Construction | 2017 |
| Elegna Care Ltd | Public administration | 2023 |
| ELEV8 Design Ltd | Construction | 2025 |
| Elevate Media 45 Limited | Professional & scientific | 2020 |
| Elevated Education Limited | Education | 2023 |
| Elisam Estates Limited | Real estate | 2019 |
| Elite Basements & Foundations Limited | Construction | 2018 |
| Elite PR Ltd | Professional & scientific | 2022 |
| Elliott Bracci Limited | Construction | 2019 |
| Elliott's Upper Crust Catering Services Limited | Hospitality | 1994 |
| Elms Collection Limited | Wholesale & retail | 2003 |
| Elnathan Investment Group Ltd | Financial services | 2025 |
| Emba Consulting Limited | Professional & scientific | 2023 |
| Embeecare Ltd | Health & social care | 2024 |
| Emberline K9 Limited | Other services | 2025 |
| Embo Destinys Venture Ltd | Health & social care | 2015 |
| EMK Building Ltd | Construction | 2025 |
| Emmay Mechanical Ltd | Construction | 2024 |
| Emperor Building Ltd | Construction | 2020 |
| Emperor Maintenance Ltd | Construction | 2020 |
| Emperor Roofing Limited | Construction | 2011 |
| Emperor Solar Ltd | Construction | 2025 |
| Emyral Builders Ltd | Construction | 2019 |
| En&S Ltd | Health & social care | 2021 |
| Encompass Insurance Brokers Limited | Financial services | 2019 |
| Encore Leisure Limited | Professional & scientific | 2019 |
| Encore Property Limited | Construction | 2019 |
| Energia INT. Ltd | Professional & scientific | 2013 |
| Energy Ease Ltd | Other services | 2014 |
| Energy Impact Limited | Administrative & support | 2007 |
| Energy Impact Water Ltd | Extraterritorial | 2017 |
| Engenius Designs Limited | Administrative & support | 2000 |
| Enhansmi Consultancy Limited | Hospitality | 2021 |
| Enjay Timber Services Limited | Construction | 1992 |
| Enrapturepro Ltd | Professional & scientific | 2022 |
| ENT Specialist Limited | Administrative & support | 2009 |
| Enterprise Smart Technologies Ltd | Administrative & support | 2001 |
| Enterprise Wide Property Limited | Real estate | 2019 |
| ENV Associates Limited | Administrative & support | 2022 |
| Eop-Pa Ltd | Professional & scientific | 2022 |
| Epsilon International London Ltd | Education | 2021 |
| Equielle Equestrian Limited | Wholesale & retail | 2021 |
| Eraphy Ltd | Real estate | 2024 |
| ERH Lift Services Ltd | Professional & scientific | 2026 |
| Ermac Tanning Studio Limited | Other services | 2024 |
| Ermine Tyres Limited | Wholesale & retail | 2015 |
| Ersin's Barbers Limited | Other services | 2018 |