| Encompass Ventures Limited | Professional & scientific | 2011 |
| Endeavour Programme UK Limited | Information & communication | 2020 |
| Endfield Goods Limited | Wholesale & retail | 2025 |
| Endless Mayhem Ltd | Wholesale & retail | 2023 |
| Endo 64 Limited | Health & social care | 2020 |
| ENH Estates Ltd | Real estate | 2026 |
| Enidamo Ltd | Hospitality | 2024 |
| Enigys Trading Limited | Energy | 2018 |
| Enjoy Education International Limited | Education | 2022 |
| Enriva Holdings Ltd | Real estate | 2026 |
| Enstore Ltd | Construction | 2022 |
| Envida Homes Ltd | Construction | 2022 |
| Environ Design (Sturry) Limited | Construction | 2009 |
| Envisage Groundcare Limited | Agriculture & fishing | 2021 |
| Epicvista Limited | Information & communication | 2024 |
| Epilepsygtx Limited | Professional & scientific | 2021 |
| Epower Engineering Services Ltd | Construction | 2023 |
| Equalangle Limited | Construction | 1986 |
| Equanimity Interiors Limited | Extraterritorial | 2022 |
| Equilibre HR Ltd | Administrative & support | 2022 |
| Equip Contract Solutions Limited | Manufacturing | 2010 |
| Equipment Supply Solutions Limited | Professional & scientific | 2012 |
| Equus Volans Ltd | Hospitality | 2026 |
| Equus Volans Ventures Ltd | Hospitality | 2026 |
| ERDEN2 Ltd | Wholesale & retail | 2026 |
| Ergan Capital Ltd | Real estate | 2018 |
| Ergan Property Limited | Real estate | 2020 |
| Ergun Besiktas Properties Ltd | Real estate | 2023 |
| Erii Food Limited | Hospitality | 2025 |
| Erkmar Limited | Professional & scientific | 2009 |
| ERM Solutions Ltd | Professional & scientific | 2021 |
| Escape Kent Ltd | Arts & recreation | 2016 |
| Esen&Co Ltd | Real estate | 2025 |
| Esposito Research & Strategy Limited | Professional & scientific | 2010 |
| Essen &Co Freehold Ltd | Real estate | 2026 |
| Essential Care Service Ltd | Health & social care | 2012 |
| Esteem Business Consultancy Limited | Manufacturing | 2023 |
| Estia Trinity Ltd | Real estate | 2025 |
| Estoneering Ltd | Professional & scientific | 2013 |
| Etch Software Ltd | Information & communication | 2016 |
| Eteknix Ltd | Information & communication | 2011 |
| Ethelbert Road Residents' Co. Limited | Households | 1995 |
| Ethereal Group Limited | Wholesale & retail | 2024 |
| Ethos Accountancy Partners Ltd | Professional & scientific | 2011 |
| Ethos Accountancy Solutions Limited | Professional & scientific | 2010 |
| Ethos Payroll Services Ltd | Professional & scientific | 2010 |
| Ethos School Improvement Limited | Education | 2012 |
| Etlco Ltd | Transport & logistics | 2023 |
| Euabalong Holdings Limited | Real estate | 2024 |
| Eudai Clinic Ltd | Health & social care | 2019 |
| Eulogy Media Ltd | Information & communication | 2007 |
| Eunice AI | Wholesale & retail | 2025 |
| Euphira Ltd | Professional & scientific | 2025 |
| Eureka Learning Systems UK LLP | | 2016 |
| Euro Property 1 Limited | Administrative & support | 2016 |
| Europatours Limited | Administrative & support | 2012 |
| European Association of Independent Research Providers Limited | Other services | 2003 |
| EV Chargers Kent Ltd | Construction | 2020 |
| Evancroft Trading Ltd | Wholesale & retail | 2026 |
| Evans Property Lets Ltd | Real estate | 2024 |
| EVDA2021 Ltd | Transport & logistics | 2023 |
| EVE MCM Ltd | Administrative & support | 2020 |
| Eventogy Ltd | Information & communication | 2011 |
| Everstar Consulting Management Ltd | Professional & scientific | 2022 |
| Everstream Ltd. | Professional & scientific | 2023 |
| Everyday Ltd | Other services | 2009 |
| Eviivo Europe Limited | Administrative & support | 2006 |
| Eviivo Limited | Information & communication | 2003 |
| EVO Finance Limited | Administrative & support | 2016 |
| Evolution Branded Clothing Limited | Manufacturing | 2010 |
| Evoque Projects Limited | Construction | 2016 |
| Excel Containers Limited | Wholesale & retail | 2014 |
| Excel Pro Ltd | Wholesale & retail | 2026 |
| Excel Trading Ltd | Water & waste | 2008 |
| Excellens Contact Lenses Limited | Wholesale & retail | 2025 |
| Exfit Ltd | Arts & recreation | 2015 |
| Experience Matters Ltd | Professional & scientific | 2024 |
| Expert Thinking (Consulting) Limited | Professional & scientific | 2017 |
| Expert Thinking Holdings Ltd | Financial services | 2023 |
| Expertvehiclesolutions Ltd | Wholesale & retail | 2025 |
| Explore Europe Limited | Administrative & support | 2015 |
| Explosive Boxing Ltd | Arts & recreation | 2024 |
| Exposis Trading Ltd | Wholesale & retail | 2023 |
| Expressaid Limited | Other services | 1986 |
| Extra Digital Limited | Information & communication | 2002 |
| Exurbia Limited | Real estate | 2009 |
| Eyelash Excellence Limited | Wholesale & retail | 2015 |
| Eyemats Limited | Professional & scientific | 2004 |
| Eyton Solutions Limited | Professional & scientific | 2009 |
| Eziflo Pumps Limited | Construction | 2024 |
| Ezri Consulting Limited | Professional & scientific | 2014 |
| F W Mansfield & SON Limited | Construction | 2022 |
| F&B Restaurant Ltd | Hospitality | 2021 |
| F. Sinclair & SON Limited | Wholesale & retail | 2025 |
| F.H. Browne & Sons (Organ Builders) Ltd | Administrative & support | 2001 |
| F.I.D. Integrated Services Ltd | Wholesale & retail | 2026 |
| F.I.S. Chemicals Holdings Limited | Financial services | 2020 |
| Fabric Construction Consultants Limited | Professional & scientific | 2022 |
| Fabric Society CIC | Information & communication | 2024 |
| Fadsam Triple OS Multipurpose Ltd | Wholesale & retail | 2025 |