| 4CIC Community Interest Company | Information & communication | 2014 |
| 4CORE Nutrition Ltd | Manufacturing | 2025 |
| 4DIMENSION Toys Ltd | Extraterritorial | 2023 |
| 4E2E Ltd | Administrative & support | 2016 |
| 4FOLD Bioscience Limited | Professional & scientific | 2025 |
| 4L Capital Limited | Financial services | 2022 |
| 4MAR C Ltd | Professional & scientific | 2026 |
| 4ME Global Private Limited | Information & communication | 2016 |
| 4T Medical Ltd | Wholesale & retail | 2012 |
| 4UBUDU Limited | Households | 2020 |
| 5 a Side Chess Limited | Education | 2015 |
| 5 Akhis Cambridge Limited | Hospitality | 2026 |
| 5 at 9 Ltd | Hospitality | 2026 |
| 5 Fitzwilliam Road Residents Association Limited | Households | 1995 |
| 5 Horizons Consulting Limited | Professional & scientific | 2021 |
| 5 New Road Harston Management Limited | Households | 2006 |
| 5 Star Facilities Management Ltd | Water & waste | 2015 |
| 50 - 74 (Even) Brackyn Road Freehold Limited | Households | 2019 |
| 50/51 Bateman Street Freehold Limited | Real estate | 2009 |
| 511 Solutions Ltd | Administrative & support | 2025 |
| 526 North Limited | Information & communication | 2013 |
| 529 Newmarket Road Limited | Real estate | 2020 |
| 556 Ltd | Manufacturing | 2025 |
| 57 High Street (Management) Limited | Households | 2015 |
| 57 ST. Philips Road Management Limited | Hospitality | 1990 |
| 57-62 Ravensworth Gardens Limited | Households | 2004 |
| 570 Newmarket Road Residential Limited | Real estate | 2015 |
| 595 Limited | Professional & scientific | 2021 |
| 5C Composites Ltd | Manufacturing | 2024 |
| 5SENSI Limited | Information & communication | 2015 |
| 5TH Axis Solutions Ltd | Manufacturing | 2025 |
| 5TH Column Studio Limited | Arts & recreation | 2022 |
| 5TH Studio Ltd | Professional & scientific | 1997 |
| 5XC Ltd | Manufacturing | 2019 |
| 615 Investments Limited | Administrative & support | 2018 |
| 615 Walnut Tree Limited | Financial services | 2023 |
| 63 Darwin Drive Holdings Ltd | Real estate | 2016 |
| 65-70 Ravensworth Gardens Limited | Real estate | 2004 |
| 66 Cambridge Road Management Limited | Households | 2009 |
| 68 Birdwood Road Ltd | Real estate | 2024 |
| 6BIT Consulting Ltd | Information & communication | 2026 |
| 7 Advisory Limited | Professional & scientific | 2024 |
| 7 of D Invest Limited | Financial services | 2020 |
| 7 of D Limited | Information & communication | 2020 |
| 718 Barber Studio Ltd | Other services | 2025 |
| 721 Genesis Ltd | Professional & scientific | 2022 |
| 740 (JC) Limited | Health & social care | 2011 |
| 740 (RH) Limited | Health & social care | 2011 |
| 78-80 Southampton Street Residents Association Limited | Households | 1983 |
| 79S Network Consulting Limited | Administrative & support | 2012 |
| 7TH Software Ltd | Information & communication | 2015 |
| 8 Cambridge Limited | Information & communication | 2024 |
| 8 Season Ltd | Wholesale & retail | 2023 |
| 8 Wired Films Limited | Arts & recreation | 2016 |
| 8, 9 and 17 Adam & EVE Court Limited | Households | 2022 |
| 80 Holland Park Management Limited | Households | 2018 |
| 80TH Atom Limited | Professional & scientific | 2013 |
| 83 Cherry Hinton Road (Cambridge) Ltd | Real estate | 2025 |
| 83 CHR Ltd | Real estate | 2025 |
| 83 Lovell Road Freehold Ltd | Real estate | 2023 |
| 8ENIGMA Limited | Professional & scientific | 2020 |
| 8POWER Limited | Professional & scientific | 2015 |
| 9 Albert Road N4 Limited | Households | 2021 |
| 9 Maitland Avenue Management Company Limited | Households | 2022 |
| 9 the Broadway Management Company Ltd | Real estate | 2021 |
| 9 to 16 ELY Place RTM Company Limited | Extraterritorial | 2025 |
| 95 Tenison Road Freehold Ltd | Households | 2024 |
| 97 Tavistock Road Flat Management Company Limited | Households | 2011 |
| A & a Chapman Limited | Wholesale & retail | 2004 |
| A & a Malaviya PVT Ltd | Health & social care | 2018 |
| A & a Services (Cambridge) Limited | Construction | 2016 |
| A & A(Uk) Limited | Administrative & support | 2022 |
| A & B Guesthouse Cambridge Ltd | Hospitality | 2016 |
| A & D Properties Cambridge Limited | Construction | 2021 |
| A & E Marquees Limited | Administrative & support | 2002 |
| A & H Upton Limited | Financial services | 2019 |
| A & K Curry Ltd | Hospitality | 2024 |
| A & K Star Ship Ltd | Manufacturing | 2026 |
| A & P Powell Holdings UK Limited | Real estate | 2024 |
| A 1 Closing Buddy Limited | Professional & scientific | 2026 |
| A a F Accountancy Ltd | Professional & scientific | 2006 |
| A B Consulting Engineers Limited | Professional & scientific | 2018 |
| A BIO Limited | Professional & scientific | 2021 |
| A Brit of Africa Ltd | Information & communication | 2019 |
| A Choudhury Ltd | Transport & logistics | 2025 |
| A Clark Landscapes & Groundworks Ltd | Administrative & support | 2024 |
| A Class Property Care Ltd | Construction | 2009 |
| A Colin Lunn (Tobacconist) Limited | Professional & scientific | 2015 |
| A DE Simone Investments Ltd | Financial services | 2013 |
| A DE Simone Ltd | Construction | 2012 |
| A Duckworth Ltd | Professional & scientific | 2020 |
| A F P Consulting Engineers Limited | Construction | 1999 |
| A G Electrical (Cambridge) Contractors Ltd | Construction | 2006 |
| A G Toft Garage Ltd | Wholesale & retail | 2015 |
| A Grant Roofing Ltd | Construction | 2004 |
| A H Orthopaedics Limited | Professional & scientific | 2011 |
| A Hauses Ltd | Real estate | 2021 |
| A Head for Work Limited | Professional & scientific | 2008 |
| A I Closing Buddy Limited | Professional & scientific | 2026 |
| A J Composites Ltd | Manufacturing | 2022 |