| Duntarvie Construction Ltd | Construction | 2025 |
| Dymond Clean Services Limited | Administrative & support | 2021 |
| D’S Nuts Ltd | Wholesale & retail | 2025 |
| E Cunningham Ltd | Administrative & support | 2024 |
| E G B R Dalton LLP | | 2018 |
| Eaglegroup Investments Limited | Real estate | 1996 |
| East Mains Motor Company Ltd | Wholesale & retail | 2018 |
| East of Scotland Drainage Services Ltd | Water & waste | 2017 |
| Eastern Holdings Limited | Professional & scientific | 1927 |
| Eastern Motor Company Limited | Wholesale & retail | 1988 |
| Eastern Properties (Scotland) Limited | Real estate | 1994 |
| Eastern Western Financial Services Limited | Extraterritorial | 1992 |
| Eastern Western Leasing Limited | Extraterritorial | 2010 |
| Eastern Western Motor Group Limited | Wholesale & retail | 1962 |
| Easy Pick UP Ltd | Transport & logistics | 2026 |
| Easy Smart Apps Ltd | Information & communication | 2026 |
| Ecko Glazing Solutions Ltd | Construction | 2026 |
| Ecosse Events Ltd | Arts & recreation | 2021 |
| Ecosse Powder Coating Ltd | Construction | 2018 |
| Ecosse Residential Property Fund LP | | 2009 |
| Ecosteel (Holdings) Limited | Financial services | 2011 |
| ED Capets Limited | Manufacturing | 2025 |
| Edin Decorating Services Ltd | Construction | 2025 |
| Edinburgh 1041 Ltd. | Extraterritorial | 2003 |
| Edinburgh Car & Van Rental Ltd | Administrative & support | 2018 |
| Edinburgh GIN Limited | Manufacturing | 2013 |
| EDM Scotland Limited | Real estate | 2020 |
| Edwards Coaching & Visuals Ltd | Arts & recreation | 2024 |
| EH Electrical Solutions (Scotland) Ltd | Manufacturing | 2024 |
| Eighties Carcrowd Ltd | Wholesale & retail | 2014 |
| Eilean Pharma Limited | Wholesale & retail | 2026 |
| Ekco Glazing Solutions Ltd | Manufacturing | 2026 |
| Electroforce & Co Ltd | Construction | 2012 |
| Elegance Within Ltd | Extraterritorial | 2016 |
| Elevated Wellness by Renée Limited | Other services | 2026 |
| Eliminate Limited | Administrative & support | 2014 |
| Elite 24/7 Transport Ltd | Transport & logistics | 2026 |
| Elite Fibre Solutions Ltd | Construction | 2020 |
| Embee Solutions Ltd | Information & communication | 2016 |
| Emkay Entertainments Agency Limited | Arts & recreation | 2022 |
| Emmg Limited | Wholesale & retail | 2026 |
| En@Grow Limited | Other services | 2021 |
| Energy Efficient Consultants Ltd | Professional & scientific | 2024 |
| Energy NL Limited | Professional & scientific | 2023 |
| EP Property Rental Ltd | Real estate | 2019 |
| ER Skin Specialist Ltd | Other services | 2023 |
| ES Session Chanters Limited | Manufacturing | 2024 |
| Euriqa Ltd | Professional & scientific | 2009 |
| European Safety Ltd | Professional & scientific | 2020 |
| Eversee Limited | Information & communication | 2026 |
| Evlv Studio Ltd | Arts & recreation | 2026 |
| EW Leasing Limited | Extraterritorial | 2010 |
| Ewfs Limited | Extraterritorial | 1994 |
| Ewosi Properties Ltd | Real estate | 2024 |
| Express Spares UK Ltd | Wholesale & retail | 2026 |
| Ezeflow United Kingdom Limited | Manufacturing | 2012 |
| Faceit Ltd | Wholesale & retail | 2025 |
| Fast Food SW Ltd | Wholesale & retail | 2018 |
| Fauna Skis Ltd | Manufacturing | 2017 |
| Faur-Seen Engineering Limited | Other services | 2011 |
| Fernhaul Limited | Transport & logistics | 2014 |
| Field and Forest Machinery Limited | Agriculture & fishing | 2015 |
| Field and Lawn Limited | Other services | 1986 |
| Final Touch Beauty Limited | Other services | 2011 |
| Financial Growth Academy Ltd | Professional & scientific | 2016 |
| Finesse Creative Ltd | Professional & scientific | 2021 |
| Fiona Mcdonald Consulting Limited | Professional & scientific | 2026 |
| First European Finance (Scotland) Limited | Administrative & support | 1992 |
| First FIX Electrical Group Ltd | Construction | 2022 |
| Fit OUT Scotland Ltd | Construction | 2023 |
| Fitness League Scotland Ltd | Arts & recreation | 2023 |
| Fitz Timber Supplies Ltd | Wholesale & retail | 2024 |
| Fivestanks Engineering Ltd | Mining & quarrying | 2016 |
| Flayva Limited | Hospitality | 2026 |
| Fleming Brothers Property Ltd | Real estate | 2024 |
| Fleximix Concrete Ltd | Manufacturing | 2012 |
| Fleximix Ltd | Extraterritorial | 2012 |
| Floweb Studio Ltd | Information & communication | 2024 |
| Food Control Consultants Ltd. | Professional & scientific | 1999 |
| Forde Training Services Limited | Education | 2009 |
| Forest Fencing (Scotland) Limited | Administrative & support | 2021 |
| Form Access Ltd | Construction | 2002 |
| Forrest Growth Partners Limited | Professional & scientific | 2024 |
| Forthvale Limited | Professional & scientific | 2000 |
| Fortis Commercial Ltd | Professional & scientific | 2022 |
| Foxburgh Limited | Extraterritorial | 1992 |
| Freehuman Ltd | Professional & scientific | 2019 |
| Fynda Ltd | Information & communication | 2026 |
| G & a Taxis Ltd. | Transport & logistics | 2001 |
| G Thomson Ltd | Administrative & support | 2024 |
| G.M.C. Public House Limited | Hospitality | 2015 |
| GA Joiners Ltd | Construction | 2016 |
| Gamblor Gaming Technologies Ltd | Information & communication | 2023 |
| Garden Mile Ltd | Wholesale & retail | 2020 |
| Garlanding Limited | Professional & scientific | 2026 |
| Garler Solutions Ltd | Professional & scientific | 2013 |
| GAS and Appliance Services Ltd | Construction | 2024 |
| GC Concrete Limited | Financial services | 2021 |
| GEH Property Investments Limited | Real estate | 2024 |
| General Practice London Limited | Health & social care | 2021 |