| 52AJ Ltd | Real estate | 2022 |
| A & R Keir Limited | Construction | 2005 |
| Abow Fish Merchant Limited | Wholesale & retail | 2021 |
| AH Fish Merchants Ltd | Wholesale & retail | 2024 |
| AH Properties (Fife) Limited | Real estate | 2022 |
| AJ Shellfish Ltd | Agriculture & fishing | 2021 |
| AL Home Improvements (Tayside) Ltd | Construction | 2023 |
| Alba Roofing Ltd. | Construction | 1998 |
| Ambrose Consultancy Limited | Professional & scientific | 2022 |
| AMD Building Services Limited | Construction | 2023 |
| Anstruther Holidays Ltd | Hospitality | 2023 |
| Ashnor Technical Services Ltd | Professional & scientific | 2026 |
| Aurora PS Consulting Ltd | Administrative & support | 2025 |
| B.R.E. Limited | Manufacturing | 1987 |
| BAS Studios Ltd | Arts & recreation | 2024 |
| Bdrc 75 Limited | Professional & scientific | 2025 |
| Bdspecialistuk Limited | Professional & scientific | 2025 |
| Bentleys Home Care Limited | Health & social care | 2023 |
| Black Events (Fife) Ltd | Hospitality | 2022 |
| Bordan Limited | Manufacturing | 1997 |
| BST Dundee Ltd | Other services | 2021 |
| Cana Holdings Ltd | Wholesale & retail | 2024 |
| CC (Fife) Limited | Hospitality | 2024 |
| CC Fish Merchant Limited | Wholesale & retail | 2021 |
| CC Hair Design Ltd | Other services | 2022 |
| CDW (Fife) Ltd | Wholesale & retail | 2024 |
| Cellar Two Limited | Hospitality | 2024 |
| CF Lettings (Fife) Ltd | Real estate | 2024 |
| Clan Well Services Ltd | Mining & quarrying | 2025 |
| Comms International Ltd | Professional & scientific | 2022 |
| Consentience Limited | Professional & scientific | 2010 |
| Corsee Ventures Ltd | Professional & scientific | 2026 |
| Crane OP Services Ltd | Other services | 2024 |
| CRS (Scotland) Limited | Real estate | 2003 |
| CT Innovative People Solutions Ltd | Professional & scientific | 2025 |
| CW Structural Engineering Ltd | Professional & scientific | 2025 |
| D.W.L. Properties Limited | Real estate | 2003 |
| DAM Business Resources Ltd | Administrative & support | 2008 |
| Ddglf Limited | Real estate | 2023 |
| Decomworks Ltd | Professional & scientific | 2025 |
| Diamond Training Solutions Ltd | Education | 2025 |
| Dundee Access Services Ltd | Construction | 2018 |
| Dundee Landscaping Company Limited | Administrative & support | 2022 |
| East Neuk Housekeeping Limited | Administrative & support | 2023 |
| East Neuk Ironing Ltd | Other services | 2023 |
| East Neuk Laundry Ltd | Other services | 2021 |
| EB (Fishing) Limited | Agriculture & fishing | 2022 |
| Ecclesgreig Estates Limited | Agriculture & fishing | 2002 |
| EDM Property Management Ltd | Real estate | 2022 |
| Enerjoe Ltd | Construction | 2025 |
| Esena Group Limited | Health & social care | 2024 |
| F&L Wells Management Ltd | Professional & scientific | 2025 |
| Ferry at Consultancy Ltd | Professional & scientific | 2022 |
| Floorsplus Limited | Construction | 2022 |
| Garden Timber Solutions (Elgin) Ltd | Construction | 2023 |
| Goldcrest Gardens Ltd | Administrative & support | 2023 |
| GP Electrical Solutions Limited | Mining & quarrying | 2025 |
| Grampian Furnishers Limited | Wholesale & retail | 2021 |
| Greasley-Harte Ltd | Professional & scientific | 2022 |
| Grillverse Ltd | Wholesale & retail | 2025 |
| GS Fish Merchants Limited | Wholesale & retail | 2022 |
| H.B. Rutherford & Co. Limited | Manufacturing | 1974 |
| Harbour Engineering (Aberdeen) Limited | Wholesale & retail | 1981 |
| Harry Lawson Haulage Limited | Wholesale & retail | 1946 |
| Harry Lawson Limited | Transport & logistics | 1964 |
| Harry Lawson Tankers Limited | Professional & scientific | 1978 |
| Harry Lawson Transport Limited | Professional & scientific | 1948 |
| HB Decorators (Dundee) Ltd | Wholesale & retail | 2021 |
| Holden Chemicals Co. | | 2001 |
| Identity of Broughty Ferry Ltd | Other services | 2023 |
| Inchbrae Roofing Ltd | Construction | 2024 |
| Inshewan Property Limited | Hospitality | 2026 |
| Inshewan Salmon Fishing Limited | Agriculture & fishing | 2026 |
| Jules by the Sea Ltd | Hospitality | 2023 |
| KD Fish Merchant Ltd | Wholesale & retail | 2022 |
| Kinneuchar Developments Ltd | Administrative & support | 2025 |
| KL Fish Merchant Limited | Wholesale & retail | 2022 |
| LA Services (Fife) Limited | Construction | 2023 |
| LAMIN8 (Scotland) Limited | Construction | 2007 |
| Lawson Trailer Rentals Limited | Transport & logistics | 1978 |
| Less O ME Slimming Ltd | Wholesale & retail | 2022 |
| Lewis Cannon Photography Ltd | Professional & scientific | 2023 |
| Links Building & Development Company Limited | Construction | 1993 |
| Llsn Ltd | Mining & quarrying | 2026 |
| LM Photography Ltd | Professional & scientific | 2022 |
| Macleod Health Ltd | Health & social care | 2023 |
| Mcdermott Piping Services Ltd | Professional & scientific | 2014 |
| MG Fish Merchant Ltd | Wholesale & retail | 2022 |
| N.A.P. Properties Limited | Real estate | 2004 |
| Newmill Asset Management Limited | Real estate | 2024 |
| Newmill Projects Limited | Professional & scientific | 2023 |
| NI Fishing Limited | Agriculture & fishing | 2023 |
| NM Access Ltd | Construction | 2024 |
| Nova Alarms Limited | Administrative & support | 2008 |
| NT Motorsport Limited | Wholesale & retail | 2022 |
| Old Mart Limited | Administrative & support | 2006 |
| P&M Electrical Fife Ltd | Construction | 2022 |
| PAX Property Ltd | Real estate | 2021 |
| Peter Davies Garden Services Ltd | Administrative & support | 2023 |
| Peter Davies Landscapes Ltd | Administrative & support | 2023 |