| Dutch Imports & Daughters Limited | Wholesale & retail | 2003 |
| E G Solar Limited | Energy | 2015 |
| E J Hairdressing Limited | Other services | 2010 |
| EARS2CLEAR Ltd | Health & social care | 2023 |
| East Coast Classics & Restorations Ltd | Wholesale & retail | 1985 |
| East Riding Property (Welton) Limited | Administrative & support | 2018 |
| East Yorkshire Logistics Ltd | Transport & logistics | 2014 |
| East Yorkshire Motor Sport Limited | Wholesale & retail | 2000 |
| East Yorkshire Towbars Ltd | Professional & scientific | 2024 |
| Ebortec Limited | Professional & scientific | 1992 |
| Ebtech Energy Systems Ltd | Construction | 2008 |
| Ebtech Engineering Ltd | Wholesale & retail | 2014 |
| Ebtech Glasshouse Systems Ltd | Construction | 2015 |
| Ebtech Holdings Limited | Wholesale & retail | 2020 |
| Eccersley Estates Ltd | Real estate | 2026 |
| Eco Engineering Installations Limited | Manufacturing | 2010 |
| Eco Uniforms Ltd | Wholesale & retail | 2025 |
| Ecoev Ltd | Construction | 2020 |
| Eddie & Joan Ltd | Wholesale & retail | 2025 |
| Eddy Property Holdings Ltd | Real estate | 2026 |
| EDE Services Limited | Construction | 2025 |
| Edgeenergyco Ltd | Wholesale & retail | 2021 |
| Edis-Nps Ltd | Information & communication | 2022 |
| Edson Investments Limited | Real estate | 2020 |
| Edward Baarda Limited | Agriculture & fishing | 1942 |
| Edwards Orthopaedics (East Yorkshire) Limited | Health & social care | 2013 |
| Efficiency Heating Co Limited | Construction | 2018 |
| Eight Acre Lake Ltd | Wholesale & retail | 2018 |
| Elan Salon East Yorkshire Limited | Other services | 2013 |
| Eldritch Studios Limited | Extraterritorial | 2023 |
| Eleanor Tomlinson Art Ltd | Arts & recreation | 2021 |
| Eleanor Tomlinson Holding Limited | Financial services | 2024 |
| Eleanor Tomlinson Property Limited | Real estate | 2024 |
| Elegy Properties Limited | Real estate | 2023 |
| Ellerker Lodge Garden Centre Ltd | Wholesale & retail | 2019 |
| Elliott Joinery Solutions Limited | Construction | 2016 |
| Elliott Property Developments Ltd | Construction | 2025 |
| Elloughton Dog Lover Limited | Other services | 2017 |
| Elloughton Greenhouses Limited | Wholesale & retail | 2020 |
| Elloughton Holdings Limited | Construction | 2023 |
| Elloughton News Limited | Wholesale & retail | 2011 |
| Elloughton Private Nursery With Little Acorns Limited | Administrative & support | 2002 |
| Emon Spice Lounge Ltd | Hospitality | 2022 |
| Empower Financial Limited | Professional & scientific | 2025 |
| ENT East Limited | Health & social care | 2025 |
| Epitome Styling Limited | Professional & scientific | 2017 |
| Equimotion Physiotherapy Ltd | Health & social care | 2025 |
| Eric Property Group Limited | Real estate | 2026 |
| Essential Nutrition Limited | Manufacturing | 1989 |
| Estb Properties Ltd | Hospitality | 2025 |
| Etrickbank Limited | Hospitality | 1951 |
| EU Editorial Services Limited | Information & communication | 2003 |
| Euromotive Ltd | Wholesale & retail | 2026 |
| Europa Crown Limited | Professional & scientific | 1990 |
| Europoort Rotterdam Ltd | Financial services | 2025 |
| Eutopia Cleaning Limited | Administrative & support | 2025 |
| Everard Group Limited | Other services | 2007 |
| Evergreen Landscaping Service Ltd | Administrative & support | 2026 |
| Evermore Electrical Ltd | Construction | 2025 |
| Evolve Site Services Ltd | Construction | 2016 |
| EX Installation & Inspection Services Ltd | Energy | 2020 |
| Exotic Properties Holdings Limited | Financial services | 2023 |
| Exotic Properties Limited | Real estate | 2023 |
| EYL Ltd | Transport & logistics | 2026 |
| Eys-Home Ltd | Wholesale & retail | 2025 |
| F a W Electrical Services Limited | Construction | 2010 |
| Factory-Fit (UK) Limited | Professional & scientific | 1993 |
| Fairhall Marketing Ltd | Professional & scientific | 2026 |
| Farspi North Ltd | Hospitality | 2025 |
| Fastway Truck and BUS Parts Ltd | Wholesale & retail | 2025 |
| FEN Property Limited | Real estate | 2022 |
| Fern Key Property Management Ltd | Real estate | 2025 |
| Fi&S Services Ltd | Real estate | 2025 |
| Figleaf 121 Limited | Real estate | 2025 |
| Figleaf Investments Limited | Administrative & support | 2008 |
| Finalprice Travel Ltd | Administrative & support | 2021 |
| Finance 4 Business Limited | Financial services | 2007 |
| Fineaf Ltd | Wholesale & retail | 2026 |
| Finished Consultancy Ltd | Construction | 2022 |
| Finvera Holdings Ltd | Financial services | 2026 |
| First Level Training Ltd | Education | 2026 |
| Flamepro Global Ltd | Manufacturing | 2017 |
| Fleet Holdings (Hessle) Limited | Construction | 1986 |
| Food Machinery Solutions Limited | Wholesale & retail | 2012 |
| Fools Guild CIC | Education | 2025 |
| Forestclean Limited | Wholesale & retail | 2023 |
| Forge Partnerships (Yorkshire) Limited | Administrative & support | 2026 |
| Fostellis Limited | Construction | 2002 |
| Frigoscandia Refrigeration Limited | Wholesale & retail | 2019 |
| Funky Schnauzer Limited | Administrative & support | 2014 |
| FUR and Feathers Pet Care Ltd | Other services | 2020 |
| Furniture Innovations and Sourcing Limited | Wholesale & retail | 2009 |
| Future Choice Services Limited | Wholesale & retail | 2025 |
| G J Waterland Limited | Administrative & support | 2012 |
| G L Rowland Ltd | Hospitality | 2025 |
| G. Purdy Anaesthetic Services Limited | Health & social care | 2002 |
| G.E. Jibson & SON Limited | Real estate | 1966 |
| Gallipots Ltd | Wholesale & retail | 2020 |
| Garry Cooper Enterprises Limited | Wholesale & retail | 2011 |
| Gary Clarkson Tax Limited | Professional & scientific | 2020 |