| Robert Holme Academy Ltd | Education | 2025 |
| Rocal Endurance Holdings Limited | Financial services | 2020 |
| Rocal Extrusions Limited | Manufacturing | 2008 |
| Rowson Agriculture Limited | Agriculture & fishing | 2011 |
| RS Potter Associates Ltd | Professional & scientific | 2026 |
| RTL (Photography) Ltd | Information & communication | 2017 |
| Rusty's (Cycles) Limited | Wholesale & retail | 1967 |
| RWC Cooling Ltd | Construction | 2015 |
| S & L Retail Ltd | Wholesale & retail | 2026 |
| S Batch Transport Ltd | Transport & logistics | 2015 |
| S Clark Homes Ltd | Real estate | 2023 |
| S Clark Plumbing & Heating Services Ltd | Construction | 2011 |
| S Davies Building Services Ltd | Construction | 2025 |
| S. Mundey, Butchers, Limited | Wholesale & retail | 1998 |
| Sagel Property Ltd | Real estate | 2023 |
| Sam's Service and Repair Limited | Wholesale & retail | 2022 |
| Samantha Cameron Studio Limited | Wholesale & retail | 2016 |
| Sanctuary Woods Limited | Information & communication | 2019 |
| Sapprouk Limited | Wholesale & retail | 2011 |
| Sazin Estates Limited | Real estate | 2024 |
| Sazin Eyecare Limited | Health & social care | 2016 |
| Sazin Healthcare UK Limited | Health & social care | 2023 |
| SC Welding Solutions Ltd | Construction | 2025 |
| Scaw-Bee Honey Ltd | Hospitality | 2025 |
| Scawbrook Care Limited | Health & social care | 2026 |
| Scawby & South Ferriby Estate Company | Agriculture & fishing | 1925 |
| Scawby Brook Electrical Ltd | Construction | 2026 |
| SDO Motor Co Ltd | Wholesale & retail | 2023 |
| Seafox Management Consultants Limited | Professional & scientific | 2010 |
| Seafront Logistics Limited | Transport & logistics | 2010 |
| Seao Properties Limited | Real estate | 2021 |
| Searby Farming Company Limited | Agriculture & fishing | 2001 |
| SELLING4EVERYONE Ltd | Wholesale & retail | 2021 |
| Sentry Financial Ltd | Professional & scientific | 2014 |
| Serenity Beauty & Aesthetics Training Ltd | Administrative & support | 2020 |
| Seton Properties Limited | Construction | 1986 |
| SFC Business Consulting Ltd | Professional & scientific | 2023 |
| Shafford Fields Livery Stud Ltd | Agriculture & fishing | 2020 |
| Shaun Wilson Limited | Construction | 2002 |
| Sheilds Holdings Limited | Professional & scientific | 2016 |
| Shire Build Ltd | Construction | 2018 |
| Shopino Ltd | Wholesale & retail | 2018 |
| Silo Facilities Management Limited | Construction | 2020 |
| Silo Maintenance Ltd | Other services | 2022 |
| Simply Land Limited | Real estate | 2015 |
| Simri Properties Limited | Financial services | 2019 |
| Sino Fish&Chips Ltd | Hospitality | 2023 |
| Sipling Covermakers Ltd. | Manufacturing | 2004 |
| Sirius Heating Solutions Ltd | Construction | 2010 |
| SJB Property Management Limited | Real estate | 2015 |
| SJP Commerce Ltd | Wholesale & retail | 2019 |
| Skylight Logistics and Sales Ltd | Transport & logistics | 2022 |
| Slate House Quarry Limited | Real estate | 2026 |
| SLH Consultancy Ltd | Professional & scientific | 2020 |
| Smith Cranes Ltd | Wholesale & retail | 2024 |
| Snake and Arrows Ltd | Financial services | 2024 |
| Social Soda Limited | Professional & scientific | 2025 |
| Sockitz Limited | Manufacturing | 2009 |
| Solarfixer Ltd | Manufacturing | 2016 |
| Solder Technology Ltd | Manufacturing | 2025 |
| Sonic Vision Labs Ltd | Information & communication | 2025 |
| SP & C Ltd | Administrative & support | 2025 |
| Specialised Sports Investments (SSI) Ltd | Professional & scientific | 2021 |
| Spencerjoneses Limited | Manufacturing | 2026 |
| Spirit Specialist Claims Services Limited | Financial services | 2015 |
| SPL Properties NO.4 Limited | Real estate | 2016 |
| Splash With Tash Ltd | Education | 2022 |
| SR Scunthorpe Limited | Wholesale & retail | 2020 |
| ST Hybald's Academy Trust | Education | 2012 |
| Star Cyclez Ltd | Wholesale & retail | 2019 |
| Star Entertainment and Catering Ltd | Hospitality | 2025 |
| Starkey's Cafe Limited | Hospitality | 2018 |
| Stars and Stiles Ltd | Hospitality | 2022 |
| Steer Solutions Ltd | Wholesale & retail | 2023 |
| Stepping Stones of Scawby | Health & social care | 2013 |
| Sterling Estate Planning Ltd | Professional & scientific | 2021 |
| Steve Ball Joinery Ltd | Construction | 1998 |
| Steve the Electrician Ltd | Construction | 2026 |
| Stones & Newstead Ltd | Real estate | 2024 |
| Stowit Storage Limited | Transport & logistics | 2004 |
| Stratos Motorsport Limited | Transport & logistics | 2000 |
| Struxa Ltd | Construction | 2025 |
| Summit (Brigg) Limited | Financial services | 2003 |
| Survival Medic Books and Training Limited | Information & communication | 2012 |
| SW Powdercoat and Cerakote Ltd | Manufacturing | 2022 |
| Swain Transport Ltd | Transport & logistics | 2010 |
| Swashbuckle CL Ltd | Real estate | 2018 |
| Swift Business Finance Limited | Administrative & support | 2026 |
| Swiftmove Services Limited | Transport & logistics | 2026 |
| Sykes Motor Services Ltd | Wholesale & retail | 2023 |
| Synergy Hospitality Solutions Ltd | Real estate | 2022 |
| T & N Vehicle Sales Limited | Wholesale & retail | 2025 |
| T & T Associates Ltd | Wholesale & retail | 2024 |
| T Brighton Engineering Services Limited | Wholesale & retail | 2003 |
| T S Truck & Plant Ltd | Wholesale & retail | 2003 |
| Tale of Two Limited | Hospitality | 2018 |
| TAN Brigg Ltd | Hospitality | 2024 |
| Tangata Services Ltd | Health & social care | 2018 |
| Tankhouse Ltd | Construction | 2021 |
| Teals Close Limited | Administrative & support | 2011 |