| Empress Quilts Limited | Manufacturing | 2023 |
| EMS Recruitment Ltd | Professional & scientific | 2021 |
| Emsn Limited | Health & social care | 2016 |
| Emtee Logistics Ltd | Transport & logistics | 2026 |
| EN AY Consultants Limited | Professional & scientific | 2023 |
| EN Guard Securities Limited | Administrative & support | 2025 |
| ENABLE2 C.I.C. | Professional & scientific | 2011 |
| ENABLE2 Translate Limited | Health & social care | 2024 |
| Enamel Bright Ltd | Wholesale & retail | 2025 |
| ENC Property Ltd | Real estate | 2021 |
| Encircle Media Group Limited | Manufacturing | 1991 |
| Enco Wool Testing Services Limited | Professional & scientific | 2017 |
| Encon Group Holdings Ltd | Financial services | 2023 |
| Encon Pharma Ltd | Administrative & support | 2023 |
| Encon Staffing Associates Ltd. | Administrative & support | 2003 |
| Encrypted Fireworks Limited | Wholesale & retail | 2023 |
| END of Day Limited | Real estate | 2004 |
| END Violence UK Ltd | Other services | 2025 |
| Endless Ends Limited | Wholesale & retail | 2025 |
| Endo Shak Limited | Education | 2022 |
| Endo Skilled Ltd | Health & social care | 2020 |
| Endure Limited | Wholesale & retail | 2014 |
| Energi Haven Ltd | Other services | 2019 |
| Energised Yorkshire Limited | Construction | 2019 |
| Energy Systems CPI Ltd | Construction | 2015 |
| Energy Unique Limited | Manufacturing | 2010 |
| Energyglaze Glass Limited | Construction | 2025 |
| Energysave Glass Limited | Construction | 2025 |
| Energywise AZ Ltd | Other services | 2022 |
| Enfield Corner Childminding Limited | Health & social care | 2026 |
| ENG Solutionzzz Limited | Wholesale & retail | 2025 |
| Engaging Communities CIC | Professional & scientific | 2015 |
| Engine Oilz Ltd | Wholesale & retail | 2025 |
| Engine Pro Ltd | Wholesale & retail | 2026 |
| Engineering Particulate Ltd | Professional & scientific | 2020 |
| Engineering Productions (Pudsey) 1987 Limited | Professional & scientific | 1943 |
| Engineering Productions (Pudsey) Limited | Manufacturing | 1987 |
| English Real Estates Limited | Professional & scientific | 1982 |
| Enhance Academy Trust | Education | 2012 |
| Enhance Medical Aesthetics Ltd | Other services | 2025 |
| Enhanced Care Pharmacy Limited | Wholesale & retail | 2023 |
| Enhancing Nature Ltd | Other services | 2025 |
| Enigma Energy Ltd | Energy | 2019 |
| Eniko Limited | Extraterritorial | 2020 |
| Enkahnz Limited | Wholesale & retail | 2007 |
| Enkahnz Smart Repair Limited | Wholesale & retail | 2014 |
| Enkahnz Training Academy | Education | 2011 |
| ENO Properties Investments Ltd | Real estate | 2024 |
| Enodo Direct Ltd | Health & social care | 2019 |
| Enrg Products Limited | Wholesale & retail | 2018 |
| Enrg-Tech Ltd | Wholesale & retail | 2014 |
| Enrich Training Consultancy Ltd | Education | 2010 |
| Ensa Limited | Other services | 2015 |
| ENT Medical Reporting Ltd | Health & social care | 2022 |
| Enterprise Partnerships Ltd | Real estate | 2024 |
| Enterprise Property Rentals Limited | Real estate | 2020 |
| Enterprise Vehicle Hire Ltd | Administrative & support | 2018 |
| Enterprise Ventilation Centre Limited | Wholesale & retail | 2011 |
| Enterprise's World Ltd | Wholesale & retail | 2025 |
| Enterprises London Store Limited | Wholesale & retail | 2026 |
| Entex Doors Limited | Administrative & support | 2009 |
| Entra Ltd | Administrative & support | 2025 |
| Envii Clothing Ltd | Wholesale & retail | 2025 |
| Enviro (UK) Limited | Manufacturing | 2011 |
| Enviro Technical Solutions (UK) Limited | Construction | 2014 |
| Enviro Water Ltd | Wholesale & retail | 2012 |
| Enviro-Air Ltd | Energy | 2012 |
| Enviro-Grab Ltd | Construction | 2024 |
| Enviroclean Solutions Limited | Transport & logistics | 2023 |
| Envirohealth Ltd | Professional & scientific | 2026 |
| Environmental Asbestos Services Ltd | Construction | 2015 |
| Environmental Control Solutions Limited | Construction | 2006 |
| Enviroplumb Group Limited | Construction | 2016 |
| Envisage Group Europe Ltd | Administrative & support | 2016 |
| Envision Windows and Doors Ltd | Construction | 2023 |
| ENZ Autoz Ltd | Wholesale & retail | 2025 |
| Enzo Housing Ltd | Real estate | 2025 |
| Enzolia Ltd | Wholesale & retail | 2024 |
| EP Healthtech Group Ltd | Financial services | 2026 |
| EP Refrigeration Limited | Manufacturing | 2001 |
| EPA Home Improvements Ltd | Construction | 2019 |
| Epanthers Limited | Wholesale & retail | 2024 |
| Epayfx Limited | Financial services | 2020 |
| EPC Assessment Services Ltd | Professional & scientific | 2025 |
| EPE Brand Management Limited | Extraterritorial | 2013 |
| EPE Distribution Limited | Professional & scientific | 2015 |
| EPE Group Limited | Financial services | 2020 |
| Ephphatha Health Care Resources Ltd | Health & social care | 2025 |
| Epic Investments Yorkshire Ltd | Real estate | 2020 |
| EPM Landscaping Ltd | Administrative & support | 2022 |
| EPN Property Limited | Real estate | 2019 |
| Epos Network Ltd | Other services | 2022 |
| EPS Legacy Holdings Ltd | Financial services | 2022 |
| Epta UK Limited | Manufacturing | 1962 |
| EQ Plumbing & DIY Ltd | Wholesale & retail | 2023 |
| Eqofluids UK Ltd | Wholesale & retail | 2015 |
| Equality Together | Health & social care | 1988 |
| Equalsii Limited | Wholesale & retail | 2021 |
| Equi Car Solutions Ltd | Administrative & support | 2025 |
| Equiflair Saddlery Ltd | Wholesale & retail | 2013 |