| 4M Property Holdings Limited | Financial services | 2020 |
| 4X4 Durham Ltd | Manufacturing | 2015 |
| AEL Automation Ltd | Manufacturing | 2024 |
| Bella Bathrooms Limited | Wholesale & retail | 2006 |
| Crestpower Limited | Professional & scientific | 2006 |
| DH6 Properties Limited | Construction | 2020 |
| DPM Property Investments Ltd | Real estate | 2024 |
| Dunelm Homes Limited | Construction | 1995 |
| Dunelm Property Services Limited | Construction | 2000 |
| ESH Acorn Homes Limited | Real estate | 2002 |
| ESH Construction Limited | Construction | 1990 |
| ESH Developments Limited | Real estate | 1993 |
| ESH Holdings Limited | Construction | 1999 |
| ESH Investments (EBT) Limited | Professional & scientific | 2015 |
| GST Transport Ltd | Transport & logistics | 2018 |
| GT Cleaning Machines Limited | Wholesale & retail | 2001 |
| HFR Group Limited | Financial services | 2024 |
| Hobkirk Brickwork Ltd | Construction | 2018 |
| Honeymans of Bowburn Ltd | Hospitality | 2020 |
| I.D.P. Masonry Limited | Professional & scientific | 2001 |
| J. Tonks (Transport) Limited | Water & waste | 1963 |
| J.B Groundworks Durham Ltd | Construction | 2022 |
| Jacs Property (North East) Limited | Hospitality | 2022 |
| JDF Transport Solutions Ltd | Transport & logistics | 2022 |
| Katem Transport Limited | Transport & logistics | 2014 |
| Kestrel (NE) Limited | Financial services | 2025 |
| Let's GET Social Ltd | Professional & scientific | 2026 |
| Lifting and Cooling Limited | Manufacturing | 2007 |
| Mathewson Limited | Financial services | 2025 |
| Metabio Solutions Ltd | Professional & scientific | 2025 |
| Neeco Design Limited | Professional & scientific | 2022 |
| Newberyl Ltd | Administrative & support | 2024 |
| Philadelphia Estates Limited | Real estate | 1996 |
| Philadelphia Properties Limited | Real estate | 1999 |
| S M Aerospace Ltd | Professional & scientific | 2025 |
| Shadow Logistics Ltd | Transport & logistics | 2024 |
| Stephen Easten Building Limited | Construction | 2006 |
| Steven Leonard Limited | Financial services | 2024 |
| Surrogate Robotics Ltd | Professional & scientific | 2024 |
| Tursdale Business Park Limited | Construction | 1999 |
| Waythrough Support Services Limited | Health & social care | 2022 |
| Wiiz Education Limited | Education | 2025 |