| 1086 Ltd | Hospitality | 2023 |
| 14 - 28 Greenacres Estate Management Company Limited | Households | 1995 |
| Adadebo Ltd | Health & social care | 2024 |
| Addy Products Limited | Professional & scientific | 1947 |
| Andrews, Kent & Stone Limited | Professional & scientific | 1993 |
| APS Building Limited | Construction | 2018 |
| BEK Sourdough Bakery Ltd | Manufacturing | 2022 |
| Book a Tyre Ltd | Wholesale & retail | 2025 |
| Botley Commercials Limited | Wholesale & retail | 2009 |
| Botley Flour Milling Company Limited | Wholesale & retail | 1921 |
| Botley Station MOT Centre Limited | Wholesale & retail | 2007 |
| Brickroots Property Group Ltd | Real estate | 2018 |
| Brownbear (Oxford) Limited | Administrative & support | 2021 |
| Clinic 95 Holdings (2) Limited | Financial services | 2024 |
| Clinic 95 Holdings Limited | Professional & scientific | 2009 |
| CRM Students Limited | Real estate | 2003 |
| Cupcake Culture Ltd | Manufacturing | 2025 |
| D Andrews Ltd | Construction | 2018 |
| Dongola Ltd | Wholesale & retail | 2025 |
| Evergreen Hair and Beauty LLP | | 2023 |
| Express Transport Ltd | Administrative & support | 2012 |
| Five Star Nails (Botley) Ltd | Other services | 2026 |
| Futurius Limited | Professional & scientific | 2013 |
| Garbo Architectural Ltd | Construction | 2019 |
| Global Medical Training Ltd. | Education | 2016 |
| Hill & Sons(Botley & Denmead)Limited | Real estate | 1946 |
| Hollybank Rest Home Limited | Hospitality | 2003 |
| Home Cleans South Ltd | Administrative & support | 2022 |
| Idmt Ltd | Health & social care | 2022 |
| Interpersona Ltd | Administrative & support | 1999 |
| Iris Surveillance Limited | Professional & scientific | 2003 |
| JMH Wheelmaster Limited | Wholesale & retail | 2009 |
| Juniper Court (Abingdon) Management Limited | Households | 1989 |
| Knights Interiors Ltd | Construction | 2014 |
| Life-Changing Dentistry Ltd | Health & social care | 2013 |
| Life-Changing Yoga Limited | Health & social care | 2021 |
| Living Well Clinical Psychology Ltd | Health & social care | 2018 |
| Long Hanborough Management Company Limited | Households | 2018 |
| M Squared Accounting Ltd | Professional & scientific | 2015 |
| ML Cars Limited | Wholesale & retail | 2023 |
| Myagentfactory Limited | Information & communication | 2025 |
| Myos Corp Ltd | Manufacturing | 2024 |
| Palak Ltd | Wholesale & retail | 2025 |
| Paramount Bathrooms Limited | Manufacturing | 2005 |
| Simon Deacon Ltd | Administrative & support | 2008 |
| Stratton Bakery Limited | Manufacturing | 1997 |
| Taggspace Ltd | Information & communication | 2011 |
| TGS Outdoors Limited | Arts & recreation | 2018 |
| THE Gilded Teapot Limited | Wholesale & retail | 2016 |
| THE Rainforester Limited | Agriculture & fishing | 2018 |
| THREE8 Services Ltd | Professional & scientific | 2019 |
| Viva Management Company (Birmingham) Limited | Households | 2006 |
| Warner Mews Management Company Limited | Real estate | 2014 |
| Westbury Gardens Management Company Limited | Households | 2003 |
| Yakushi Events Ltd | Hospitality | 2019 |
| ZAK Long Ltd | Professional & scientific | 2019 |