| Trial House Ltd | Health & social care | 2022 |
| Tricia B Shellfish Ltd | Agriculture & fishing | 2011 |
| Trident Sports Events Limited | Arts & recreation | 2021 |
| Trinity Place (Corporate Theatre) Limited | Administrative & support | 2004 |
| Triumphant Marketing Ltd | Wholesale & retail | 2023 |
| Triumphant Records Ltd | Wholesale & retail | 2023 |
| Trojan Fire Ltd | Construction | 2026 |
| Trph Clini Services Ltd | Health & social care | 2025 |
| Truck Solutions Ltd | Wholesale & retail | 2025 |
| Truckrent Limited | Transport & logistics | 2007 |
| True Healthcare Group Limited | Health & social care | 2017 |
| True Value Autos Ltd | Wholesale & retail | 2023 |
| Truride Ltd | Transport & logistics | 2025 |
| Trustees of Boston University | | 1993 |
| Trustees of Boston University | | 2022 |
| TS Trading Co Limited | Agriculture & fishing | 1990 |
| TSK Retail Limited | Wholesale & retail | 2024 |
| Tudlex Ltd | Transport & logistics | 2022 |
| Tulip Business & Consulting Services Limited | Administrative & support | 2010 |
| Tulley Engineering (Services) Ltd | Professional & scientific | 2022 |
| Tumble Totz Limited | Arts & recreation | 2016 |
| Tunnard Estates Limited | Agriculture & fishing | 1988 |
| Tunnard Farms Limited | Agriculture & fishing | 2000 |
| Tunnard Ltd | Agriculture & fishing | 2024 |
| Tunnard Straw Ltd | Agriculture & fishing | 2021 |
| Tunnel Bank Farming Company Limited | Agriculture & fishing | 1998 |
| Tuplin and SON (Farmers and Produce Merchants) Limited | Agriculture & fishing | 1976 |
| Turcu Trans Limited | Transport & logistics | 2026 |
| Turnstone Property Management Limited | Construction | 1964 |
| Twenty Hotel and Bar (Boston) Ltd | Hospitality | 2018 |
| Tyrah Sinclair Media and Marketing Ltd | Professional & scientific | 2025 |
| Tyre Processors Ltd | Water & waste | 2008 |
| Tytton Court Management Limited | Real estate | 1988 |
| UHN Fastfood Ltd | Hospitality | 2025 |
| UK Adventure Store Limited | Extraterritorial | 2016 |
| UK Fresh Produce Network Ltd | Other services | 2025 |
| UK International College of Aesthetics Ltd | Education | 2025 |
| UK Lancaster Projects Limited | Construction | 2025 |
| UK MGE Limited | Construction | 2021 |
| UK Nails Boston Ltd | Other services | 2024 |
| UK Recruitment Agency Limited | Administrative & support | 2009 |
| Ukpaper Limited | Wholesale & retail | 2014 |
| Underdog Restoration & Speed Shop Ltd | Extraterritorial | 2024 |
| Underwing Ltd | Professional & scientific | 2018 |
| Unique Hope CIC | Health & social care | 2026 |
| Unit Centre Boston Limited | Real estate | 2026 |
| Unit Hub BOS Ltd | Wholesale & retail | 2026 |
| United House Limited | Health & social care | 2019 |
| Unplugged Recycling | Water & waste | 2002 |
| Untouched Global Ltd | Arts & recreation | 2023 |
| UP to the 9S Limited | Wholesale & retail | 2017 |
| Upsalls Drylining Ltd | Construction | 2019 |
| Upstream Systems, Inc | | 2000 |
| Urban Fresh 63 Ltd | Hospitality | 2024 |
| Utility Estates Limited | Real estate | 2011 |
| Utmuk Ltd | Wholesale & retail | 2025 |
| V&V Logistics & Rentals Ltd | Transport & logistics | 2019 |
| V.Messinger & Sons Limited | Real estate | 1963 |
| Vaiku Darzelis Ltd | Transport & logistics | 2026 |
| Vaima Transport Ltd | Transport & logistics | 2018 |
| VAL Okechukwu Medics Ltd | Health & social care | 2023 |
| Vale Road (Spilsby) Management Company Limited | Households | 2019 |
| Valitech Limited | Information & communication | 2022 |
| Vality Technology Incorporated | | 1998 |
| Vampo (Holdings) Ltd | Administrative & support | 2016 |
| Van WYK Perfect Smile Limited | Health & social care | 2013 |
| Van-Tastic Cryogenics Ltd | Professional & scientific | 2021 |
| Vanamali International Ltd | Information & communication | 2015 |
| Vanilla Reindeer Limited | Wholesale & retail | 2017 |
| Vanity Hair Locks and Looks Limited | Wholesale & retail | 2019 |
| VANS4LIFE Ltd | Wholesale & retail | 2025 |
| Vantage Property Management Ltd | Hospitality | 2020 |
| Vapesquad Limited | Wholesale & retail | 2017 |
| Vaporvm UK Ltd | Information & communication | 2021 |
| Variability Consultancy Limited | Information & communication | 2025 |
| Vasile Sotir Ltd | Transport & logistics | 2024 |
| Vasy Transport UK Limited | Transport & logistics | 2015 |
| Vaughan Dent Developments Ltd | Construction | 2013 |
| Vauxhall Properties Limited | Real estate | 2024 |
| VC Logistics and Transports Ltd | Transport & logistics | 2020 |
| VDM 777 Limited | Wholesale & retail | 2015 |
| Vegan Vegetables Limited | Agriculture & fishing | 2021 |
| Velocity Consultancy Limited | Professional & scientific | 2024 |
| Veneer Consult Limited | Administrative & support | 2015 |
| Venkat Property Limited | Real estate | 2024 |
| Venture Offices Limited | Professional & scientific | 2015 |
| Vere Bros (Contractors) Limited | Construction | 2008 |
| Verfresh - Trading Limited | Wholesale & retail | 2013 |
| Verity Property Limited | Real estate | 2025 |
| Vesync (UK) Limited | Wholesale & retail | 2021 |
| Vgsi Limited | Wholesale & retail | 2025 |
| Viant International Corporation | | 1999 |
| Vibrant Spice Pizza Ltd | Hospitality | 2023 |
| Vical Services Ltd | Construction | 2019 |
| Vicarage Truck Rentals Limited | Administrative & support | 2011 |
| Vickers Services Ltd | Real estate | 2020 |
| Vida Transpor Limited | Transport & logistics | 2021 |
| Vidas Transport Ltd | Transport & logistics | 2023 |
| Videima Limited | Transport & logistics | 2022 |
| Viki-Trans 14 Ltd | Transport & logistics | 2018 |