| Riana Gold Ltd | Wholesale & retail | 2024 |
| Rice & Spice Boston Ltd | Hospitality | 2017 |
| Richard Austin Agriculture Limited | Professional & scientific | 2009 |
| Richard Hardy (Fishtoft) | Agriculture & fishing | 1950 |
| Richard M Barnes Limited | Manufacturing | 2015 |
| Richard Teare Limited | Other services | 2021 |
| Ricken Ltd | Transport & logistics | 2019 |
| Ridlington Day Centre Ltd | Health & social care | 2012 |
| Rido Motors Limited | Wholesale & retail | 2017 |
| Riffler Ltd | Information & communication | 2023 |
| Rimantas and SON Limited | Wholesale & retail | 2018 |
| Rimi Limited | Manufacturing | 2023 |
| Ringrose Law Limited | Extraterritorial | 2013 |
| Rinku & Kakoo Limited | Real estate | 2022 |
| Rio-Hair Ltd | Other services | 2024 |
| Ritzy Auto Ltd | Wholesale & retail | 2021 |
| Riverside Property Care Ltd | Construction | 2025 |
| RJE Media Ltd | Other services | 2019 |
| RJH Supplies Limited | Wholesale & retail | 2015 |
| RK Hobson and Sons Limited | Agriculture & fishing | 2007 |
| RKT Homes Limited | Construction | 2025 |
| RLF Holding Ltd | Real estate | 2024 |
| RM Textiles Ltd | Wholesale & retail | 2026 |
| RMP Traders Ltd | Administrative & support | 2025 |
| RMS Building & Groundwork Ltd | Construction | 2024 |
| RMS Surveys Ltd | Professional & scientific | 2023 |
| RNH Property Ltd | Real estate | 2024 |
| ROB Way Ltd | Transport & logistics | 2025 |
| Rob-Otix AI Ltd | Information & communication | 2020 |
| Robert Collin Limited | Agriculture & fishing | 2012 |
| Robert H. Crawford & SON | Other services | 1956 |
| Robert Services Limited | Transport & logistics | 2007 |
| Rocdaymer & Co Ltd | Professional & scientific | 2020 |
| Rock Dove Limited | Other services | 2010 |
| Rockingham Occupational Health Limited | Health & social care | 2015 |
| Roger Budge (Electrical Engineering) Limited | Construction | 1974 |
| Rogue With Love Ltd | Wholesale & retail | 2026 |
| Rojo Jims Ltd | Transport & logistics | 2023 |
| Rolec Services Limited | Manufacturing | 1988 |
| Rollground Limited | Households | 1991 |
| Roman&Company Limited | Wholesale & retail | 2024 |
| Ronin Renovations Limited | Construction | 2025 |
| Rosanne House Property Ltd | Real estate | 2022 |
| Rosebery Homes Limited | Construction | 2021 |
| Roskell Kime & Co Limited | Professional & scientific | 2025 |
| Ross Alexander Property Investment Ltd | Real estate | 2016 |
| Roughton Estates Limited | Real estate | 2013 |
| Roughton Farms Limited | Agriculture & fishing | 1954 |
| Rowe Properties Ltd | Real estate | 2017 |
| ROX Salons Limited | Other services | 2013 |
| ROY Dent Limited | Administrative & support | 2023 |
| Royce Maintenance Services Ltd | Construction | 2025 |
| Royle Lakes Limited | Hospitality | 2008 |
| Roy’S Medical Ltd | Health & social care | 2023 |
| RP Boston Limited | Extraterritorial | 2019 |
| RR Consultancy Ltd | Financial services | 2019 |
| RR Investments Ltd | Real estate | 2022 |
| RS Refrigeration Limited | Manufacturing | 2021 |
| RS Service Boston Ltd | Construction | 2017 |
| RT Electrical Solutions Ltd | Construction | 2025 |
| Rudd Construction Services Limited | Construction | 2013 |
| Rudd Electrical Limited | Construction | 2016 |
| Rukh-E-Shaida Ltd | Health & social care | 2023 |
| Rundles International Limited | Manufacturing | 2000 |
| Russell Taylor Recycling Ltd | Water & waste | 2006 |
| Rustransport Ltd | Administrative & support | 2017 |
| RV Retail Ltd | Wholesale & retail | 2019 |
| Ryan Stanley Ltd | Wholesale & retail | 2019 |
| Ryband Flowers and Plants Ltd | Wholesale & retail | 2014 |
| Rylott Films Ltd | Information & communication | 2019 |
| S & L Seasons Ltd | Wholesale & retail | 2024 |
| S & P Clarke Holdings Ltd | Financial services | 2019 |
| S & P Clarke Limited | Real estate | 2019 |
| S Brothers Property Limited | Real estate | 2025 |
| S Dennington Construction Ltd | Construction | 2016 |
| S G Services (Boston) Ltd | Transport & logistics | 2009 |
| S J Tetther Limited | Construction | 2018 |
| S Leggate & Sons Limited | Agriculture & fishing | 2012 |
| S R Coldrooms Limited | Manufacturing | 2019 |
| S Shahwe Ltd | Health & social care | 2016 |
| S Thorpe Transport Ltd | Transport & logistics | 2022 |
| S&C Statham Ltd | Hospitality | 2024 |
| S. T. Portess & SON Ltd | Construction | 2015 |
| S.John Stevens Memorial Limited | Arts & recreation | 2011 |
| S.L.H Boston Ltd | Transport & logistics | 2014 |
| S.M.G. Management Company Limited | Real estate | 1987 |
| S.R.D Haulage Solutions Ltd | Transport & logistics | 2020 |
| S.Roberts & Sons Limited | Agriculture & fishing | 1957 |
| Sack Store Limited | Wholesale & retail | 1983 |
| Sack Store Stamford Limited | Extraterritorial | 2014 |
| Sadie Wilson VA Ltd | Administrative & support | 2022 |
| SAF Haulage Limited | Transport & logistics | 2019 |
| Safe Flame GAS Services Limited | Construction | 2017 |
| Saidi Trans Limited | Transport & logistics | 2025 |
| Saifullah Ilyas Limited | Wholesale & retail | 2025 |
| Sail Close Managers Limited | Households | 2026 |
| Sakata UK Limited | Wholesale & retail | 1993 |
| Sakura Bar Ltd | Hospitality | 2024 |
| Salama Enterprise Ltd | Information & communication | 2026 |
| Salimabad Ltd | Hospitality | 2018 |