| Dynace Global UK Limited | Wholesale & retail | 2024 |
| Dynamix Elite Properties Ltd | Real estate | 2024 |
| Dynamix Hair Design Limited | Other services | 2015 |
| DYO Trans Limited | Transport & logistics | 2017 |
| E & C B MAY Limited | Hospitality | 2015 |
| E & D Services Ltd | Construction | 2020 |
| E Clayton & Sons Limited | Agriculture & fishing | 2007 |
| E M Bush Limited | Agriculture & fishing | 2020 |
| E&G Renovation Service Ltd | Construction | 2021 |
| E&J Alex Enterprise Ltd | Transport & logistics | 2018 |
| E.F.W. Ltd. | Manufacturing | 1996 |
| E.M.Kitchen Services Ltd | Hospitality | 2026 |
| EAP Transport Limited | Transport & logistics | 2016 |
| Earlsgate Group Limited | Wholesale & retail | 2018 |
| East Anglian Foods Ltd | Wholesale & retail | 2007 |
| East Anglian Piling (Boston) Limited | Construction | 2009 |
| East Anglian Piling Ltd | Construction | 2018 |
| East Coast ARB Ltd | Agriculture & fishing | 2023 |
| East Coast Discount Fishing Tackle Ltd | Other services | 2017 |
| East Coast Diving Limited | Arts & recreation | 2012 |
| East Coast Industries Limited | Construction | 2013 |
| East Kirkby Engineering Co Limited | Manufacturing | 2000 |
| East Kirkby Refrigeration and Air Conditioning Limited | Energy | 2000 |
| East Lincolnshire Broadcasting Community Interest Company | Information & communication | 2022 |
| East Lincolnshire Seafoods Ltd | Wholesale & retail | 2026 |
| East Lincs Smart Repair Ltd | Manufacturing | 2012 |
| East Midlands Consultants Ltd | Administrative & support | 2017 |
| East Midlands Pest Control Limited | Agriculture & fishing | 2010 |
| Eastcoast Kitchens Limited | Construction | 2006 |
| Eastcoast Leisure Operations Ltd | Wholesale & retail | 2021 |
| Eastern Enterprises Limited | Administrative & support | 1989 |
| Eastfield Guest House Limited | Hospitality | 2002 |
| Easytech and Gifts Ltd | Wholesale & retail | 2026 |
| EB IT Support Ltd | Other services | 2023 |
| Ecare (Call Support) Limited | Health & social care | 2024 |
| Eco Biomass (UK) Limited | Wholesale & retail | 2022 |
| Eco Bulk Ltd | Wholesale & retail | 2024 |
| Eco Electrical Solutions Limited | Construction | 2011 |
| Eco Freeze Ltd | Wholesale & retail | 2016 |
| Ecoflow Ltd | Construction | 2018 |
| Ecolearn CIC | Education | 2021 |
| Ecopure Europe Limited | Wholesale & retail | 2015 |
| Ecrin (Kirton) Ltd | Wholesale & retail | 2019 |
| Eden Business Holdings Limited | Financial services | 2024 |
| Eden Business Park Limited | Real estate | 2024 |
| Eden Quality Management and Inspection Limited | Professional & scientific | 2022 |
| Edore Housing Limited | Real estate | 2023 |
| Edore Science Consulting Ltd | Other services | 2023 |
| Edward Pearson Ltd | Transport & logistics | 2022 |
| EEU in U.K. Community Support Ltd | Administrative & support | 2022 |
| EG Sewing Services Ltd | Manufacturing | 2020 |
| Eggmonkey Lincoln Ltd | Arts & recreation | 2024 |
| Eggmonkey UN Limited | Arts & recreation | 2024 |
| EGS Transport Limited | Transport & logistics | 2023 |
| EHD Cars Limited | Wholesale & retail | 2017 |
| Ejfirstcarpentry Limited | Manufacturing | 2025 |
| EK Cargo Ltd | Transport & logistics | 2025 |
| EKA Trans Ltd | Transport & logistics | 2019 |
| EKA Transport Ltd | Transport & logistics | 2019 |
| Ekins Security Services Ltd | Administrative & support | 2019 |
| EL Burrito Ltd | Hospitality | 2024 |
| Elderberry House T/A the Three Horseshoes Leverton Limited | Hospitality | 2025 |
| Elderhostel, Inc. | | 2019 |
| Electrical Distribution Services Ltd | Construction | 2009 |
| Elena Cleaning 2024 Ltd | Administrative & support | 2024 |
| Elevate Controls Ltd | Professional & scientific | 2025 |
| Elevate Services Group Ltd | Construction | 2026 |
| Elevate With EM Limited | Professional & scientific | 2026 |
| Elgro Potatoes Limited | Wholesale & retail | 2001 |
| Elise Joy Limited | Professional & scientific | 2025 |
| Elite Instruments Ltd | Wholesale & retail | 2023 |
| Elite Windscreen Repair Ltd | Wholesale & retail | 2023 |
| Elkephant Human Data Ltd | Professional & scientific | 2023 |
| Elkington Mckay Limited | Professional & scientific | 2014 |
| Ellem Services Ltd | Construction | 2018 |
| Ellen Cramoysan's Occupational Therapy Practice Ltd | Health & social care | 2023 |
| ELM Management Company Limited | Households | 1985 |
| Elvis Rogers Ltd | Administrative & support | 2025 |
| EMA and Aironas Limited | Wholesale & retail | 2025 |
| Emberton Imperial Ltd | Wholesale & retail | 2020 |
| Emilitek Enterprises Ltd | Information & communication | 2020 |
| Emilly Luxury Cleaning Services Ltd | Administrative & support | 2026 |
| Empire Transport Ltd | Transport & logistics | 2019 |
| Empirical Business Solutions Ltd | Administrative & support | 2021 |
| Empress Avenue 1 Ltd | Real estate | 2022 |
| Empress Avenue 2 Ltd | Real estate | 2022 |
| Emsway Homes Ltd | Construction | 2022 |
| Enclave Cabinetry Limited | Manufacturing | 2025 |
| Endeavour Radio Ltd | Information & communication | 2008 |
| Enderby Creations Limited | Information & communication | 2017 |
| Energy Efficient Drive Systems Ltd | Manufacturing | 2021 |
| Engineershub Ltd | Information & communication | 2026 |
| Entrance Matting Systems Limited | Manufacturing | 2000 |
| Entry Solutions Locksmiths Limited | Other services | 2025 |
| Envirotyre Holdings Limited | Financial services | 2026 |
| Envirotyre UK Limited | Water & waste | 2002 |
| Epton Joinery & Construction Ltd | Construction | 2025 |
| Equine Careers Limited | Extraterritorial | 2014 |
| Equine Sense Limited | Wholesale & retail | 2016 |
| Erginta Limited | Transport & logistics | 2017 |