| Primaris Ltd | Information & communication | 2024 |
| Prime Finish Ltd | Manufacturing | 1984 |
| Project 21 Detail (Edinburgh) Ltd | Administrative & support | 2025 |
| Quantum Commercial Management (Scotland) Limited | Professional & scientific | 2024 |
| Ramblin Roast Ltd | Hospitality | 2024 |
| Rbpetservices Ltd | Wholesale & retail | 2025 |
| Reaper Asset Management Ltd | Wholesale & retail | 2021 |
| Reference Point (Europe) Limited | Administrative & support | 2005 |
| Reliaheat Ltd | Construction | 2020 |
| Removal Services Lothians Ltd | Transport & logistics | 2026 |
| Reseng Consultants Ltd | Mining & quarrying | 2014 |
| REV UP Limited | Transport & logistics | 2024 |
| Revvive Motors Limited | Wholesale & retail | 2026 |
| RJ1875 Ltd | Professional & scientific | 2018 |
| Rntachef Mobile Catering Limited | Hospitality | 2025 |
| Robbins Taxis | | 1998 |
| Rosehill Care Home Dalkeith Ltd | Administrative & support | 2024 |
| Rosemar Limited | Professional & scientific | 2017 |
| Ross Armstrong GAS Central Heating & Plumbing Services Limited | Construction | 2015 |
| Ross's of Edinburgh Ltd | Manufacturing | 2015 |
| RW Building and Construction Management Ltd | Construction | 2015 |
| S & M PIA Holdings Ltd | Real estate | 2025 |
| S.N.G. Control Systems Limited | Manufacturing | 1973 |
| Sarwani Investments Ltd | Real estate | 2024 |
| Scaussie Investments Ltd | Real estate | 2023 |
| Scotist Ltd | Wholesale & retail | 2016 |
| Scotpal Ltd | Education | 2018 |
| SCS BIN Services Ltd | Administrative & support | 2019 |
| SCS Cleaning Services Ltd | Administrative & support | 2008 |
| Shah Brother Properties Ltd | Real estate | 2025 |
| Sharp & Wooton Properties Limited | Real estate | 2025 |
| Shutter Scotland Limited | Administrative & support | 2025 |
| Simply Controlled Ltd | Manufacturing | 2009 |
| Skin Story Aesthetics Ltd | Other services | 2020 |
| SL Fitness & Recovery Ltd | Health & social care | 2022 |
| Smart Hire Recruitment Solutions Ltd | Administrative & support | 2019 |
| Smartlingo Ltd | Education | 2024 |
| Snappy Rentals Limited | Administrative & support | 2018 |
| Snkw Ltd | Wholesale & retail | 2022 |
| Solidum Fautor Ltd | Wholesale & retail | 2018 |
| Specialist Access Technicians Limited | Mining & quarrying | 2013 |
| Sphynx Group Ltd | Administrative & support | 2026 |
| Spinlock Sierra Systems Consulting Limited | Information & communication | 2007 |
| Square Face Productions Limited | Information & communication | 2006 |
| SS Kitchens Ltd | Hospitality | 2025 |
| SSB Bonnyrigg Ltd | Other services | 2022 |
| Starlight Coatings Ltd | Other services | 2017 |
| Start Bright Nursery Bonnyrigg Ltd | Education | 2016 |
| Streamline Cycling Ltd | Wholesale & retail | 2018 |
| Study Loom Ltd | Education | 2024 |
| Sunny Telecomms Ltd | Manufacturing | 2015 |
| Surface Tech Ltd | Other services | 2018 |
| Tamilan Ltd | Wholesale & retail | 2014 |
| Tandon Properties Limited | Real estate | 2024 |
| Tasien Sardar Taxis Limited | Transport & logistics | 2014 |
| Taskforce (Finishing & Handling) Limited | Manufacturing | 1989 |
| Taylor Maid Holidays Ltd | Real estate | 2025 |
| Tennant M Limited | Wholesale & retail | 2025 |
| THE Car Cave (Scotland) Ltd | Wholesale & retail | 2016 |
| THE Clan and Cask Ltd | Hospitality | 2025 |
| THE Clan and CASK2 Ltd | Hospitality | 2025 |
| THE Crystal Cave Edinburgh Limited | Wholesale & retail | 2022 |
| THE Devil IS in the Details Business Analysis Limited | Information & communication | 2024 |
| THE Energy Engineers Scotland Ltd | Energy | 2019 |
| THE Grenadine MAN Limited | Construction | 2025 |
| THE Handy Gardener (Scotland) Ltd | Administrative & support | 2022 |
| THE Mortgage & Finance Company Limited | Financial services | 2018 |
| THE Wildest One Trading Ltd | Wholesale & retail | 2025 |
| Thistle Yarns Ltd | Manufacturing | 2025 |
| Thistle&Thyme Garden Care LLP | | 2024 |
| Ticketyboo (Scotland) Ltd | Professional & scientific | 2024 |
| Tily Blooms Ltd | Wholesale & retail | 2022 |
| Time & Wealth Creator Limited | Other services | 2019 |
| Toliman Limited | Information & communication | 2005 |
| TSL Services (Scotland) Ltd | Agriculture & fishing | 2016 |
| Turn 2 Learn Limited | Education | 2025 |
| Tyre Assist Scotland Ltd | Wholesale & retail | 2020 |
| United Private Hire Ltd | Transport & logistics | 2018 |
| Vacation Rentals Scotland Ltd | Hospitality | 2010 |
| Vaqqy Ltd | Administrative & support | 2023 |
| Vero True Image Ltd | Wholesale & retail | 2025 |
| Waugh Electrical Ltd | Manufacturing | 2020 |
| Waverley Accountancy Limited | Professional & scientific | 2017 |
| Weatherburn Oak Ltd | Information & communication | 2025 |
| Wedding Warehouse UK Ltd | Wholesale & retail | 2025 |
| Wightman Lettings Ltd | Real estate | 2024 |
| William Fraser Developments Ltd | Construction | 2021 |
| Willsweep Ltd. | Administrative & support | 1999 |
| Xmass Properties Ltd | Real estate | 2022 |