| 8 O NI - 8 of US Limited | Agriculture & fishing | 2016 |
| AJ Properties Bodorgan Limited | Real estate | 2022 |
| AM Drainage Ltd | Water & waste | 2024 |
| Anglesey Beach & Resin Art Limited | Administrative & support | 2013 |
| Anglesey Biodiversity Limited | Agriculture & fishing | 2024 |
| Anglesey Upholstery Limited | Manufacturing | 2019 |
| Anglo Chinese Food Corporation Ltd | Wholesale & retail | 2013 |
| Aros MÔN CYF | Real estate | 2023 |
| Bodorgan Properties (UK) Limited | Real estate | 2018 |
| BRO Aberffraw Alliance CIC | Administrative & support | 2025 |
| Cambrian Planning and Development Ltd | Professional & scientific | 2018 |
| Canolfan HEN Ysgol Bodorgan CYF | Other services | 2006 |
| Capel Sardis Property Management Ltd | Households | 2022 |
| Chilly Ltd | Information & communication | 2017 |
| Cwrw Y COB Ltd | Wholesale & retail | 2025 |
| Eterrna Ltd | Other services | 2026 |
| Fircone Estates Limited | Professional & scientific | 2003 |
| First Energy Corporation UK Ltd | Wholesale & retail | 2025 |
| G Roberts Roofing Limited | Construction | 2025 |
| Gwasg Acherantia CYF | Education | 2021 |
| H for Hydrogen Ltd | Manufacturing | 2023 |
| Iolo Owen & Co. Limited | Agriculture & fishing | 1974 |
| JC Landscape & Garden Services Ltd | Administrative & support | 2016 |
| Jnho Property Limited | Real estate | 2019 |
| Lily's Logs Ltd | Wholesale & retail | 2025 |
| Llywelyn Financial Ltd | Professional & scientific | 2011 |
| M & K Developments LLP | | 2009 |
| Maelog Jerseys Ltd | Agriculture & fishing | 2020 |
| Matopos Computing Limited | Information & communication | 1997 |
| Medwyns' of Anglesey Garden Products Limited | Agriculture & fishing | 2009 |
| Mermaid Holidays Limited | Hospitality | 2023 |
| Mezu Limited | Wholesale & retail | 2011 |
| Morgan Owen A'I Gwmni CYF | Professional & scientific | 2024 |
| Nova Chrome UK Limited | Wholesale & retail | 2002 |
| Paradwys CYF | Real estate | 2012 |
| Perkins and Sons Limited | Construction | 2015 |
| Perkins and Sons Storage Limited | Administrative & support | 2026 |
| Pristine Cleaning Services NW Ltd | Administrative & support | 2026 |
| Pristine Property Services NW Ltd | Administrative & support | 2025 |
| Property Options Ltd | Households | 2025 |
| Raltech Limited | Manufacturing | 2013 |
| Rhos CYF | Health & social care | 2013 |
| Rhos Properties Limited | Real estate | 2019 |
| Social Hour Coffee Roasters Ltd | Manufacturing | 2023 |
| Subsea Engineering Services Limited | Construction | 2012 |
| T. Owen Electrical Ltd | Construction | 2018 |
| THE Linen & Laundry Company NW Ltd | Administrative & support | 2026 |
| UXO Specialist Ltd. | Construction | 2023 |
| W & O Construction Ltd | Construction | 2024 |
| W Glynne Owen & Co Limited | Professional & scientific | 2002 |
| WCD Building Ltd | Construction | 2022 |
| WCD MON Ltd | Real estate | 2021 |
| Williams & Gwilym Limited | Agriculture & fishing | 2006 |