| Scotbev Supplies Limited | Wholesale & retail | 2016 |
| Scott Mcewan Builders Limited | Administrative & support | 2001 |
| SDC Electrical Services Limited | Extraterritorial | 2008 |
| Secret Paths Ltd | Administrative & support | 2025 |
| Sensitive Hounds Ltd | Wholesale & retail | 2025 |
| Seven G Engineering Ltd | Professional & scientific | 2018 |
| SG Property Box Ltd | Real estate | 2025 |
| SIM Homes Group Ltd | Real estate | 2026 |
| Simtel Solutions Ltd | Information & communication | 2022 |
| Skellyman Games Ltd | Information & communication | 2022 |
| Smac Stirling Limited | Hospitality | 2026 |
| Smith & Leishman Limited | | 1935 |
| Smith - Barr Limited | Real estate | 2018 |
| Sneddon and Sons Ltd | Construction | 2026 |
| Snore Seal Ltd | Wholesale & retail | 2025 |
| Solid Ground Ventures Ltd | Real estate | 2026 |
| Sophie Brewster Coaching Limited | Education | 2024 |
| Sosin Joinery Limited | Construction | 2019 |
| SPG Brickworx Limited | Construction | 2016 |
| SPG Estates Limited | Real estate | 2019 |
| Squeaky Clean Window Cleaning Services Limited | Administrative & support | 2018 |
| SRL007 Property Limited | Administrative & support | 2025 |
| Stav Kebab Ltd | Hospitality | 2022 |
| Stewart Resources Ltd | Manufacturing | 2023 |
| Strive Heating Limited | Construction | 2025 |
| Stuffs 2 U Retail Ltd | Wholesale & retail | 2015 |
| Subtle Perfection Aesthetic Studio Ltd | Education | 2026 |
| Sukate Property Limited | Real estate | 2025 |
| Surma Dunfermline Ltd | Hospitality | 2025 |
| SZ Trans Ltd | Transport & logistics | 2022 |
| Talking Heads 84 Ltd | Other services | 2023 |
| Tape Boys UK Ltd | Construction | 2025 |
| Taylors Creative Living Ltd | Wholesale & retail | 2023 |
| Techniche Production Ltd | Administrative & support | 2023 |
| Teetotal Fitness Ltd | Other services | 2025 |
| Tenement Maintenance Services Ltd | Construction | 2025 |
| Tgfit Coaching Limited | Education | 2025 |
| THE Hive Scotland Ltd | Wholesale & retail | 2023 |
| THE Little Bairns Co Limited | Manufacturing | 2021 |
| THE Mcguinness Family Company Limited | Professional & scientific | 2016 |
| THE Swole Coach Ltd | Education | 2022 |
| THE Toon Travel Ltd | Administrative & support | 2019 |
| THE Wallpaper Mural Company Scotland Limited | Wholesale & retail | 2014 |
| THE Warehouse Salon & Academy Ltd | Education | 2023 |
| Theo B Racing Ltd | Wholesale & retail | 2025 |
| Thomas Burns & SON Limited | Wholesale & retail | 2017 |
| Time for YOU West Lothians Limited | Other services | 2014 |
| TOO Rough Films Ltd | Information & communication | 2020 |
| Twotouch Coaching Limited | Professional & scientific | 2022 |
| Typemenu Ltd | Information & communication | 2025 |
| Uaap Trading Limited | Wholesale & retail | 2022 |
| UK Geomatics Limited | Professional & scientific | 2024 |
| Vallumtech Ltd | Information & communication | 2020 |
| Vapour X Limited | Wholesale & retail | 2014 |
| Velev Consulting Ltd | Professional & scientific | 2020 |
| Vice Consultancy Limited | Administrative & support | 2025 |
| Virtus Physique Coaching Limited | Health & social care | 2022 |
| Visabridge Ltd | Professional & scientific | 2026 |
| Wala Haulage Ltd | Transport & logistics | 2021 |
| Wali & Co (Scotland) Ltd | Wholesale & retail | 2019 |
| Water Drainage Services Ltd | Construction | 2016 |
| Westmuir Limited | Professional & scientific | 2001 |
| WHM Plumbing & Heating Limited | Construction | 2008 |
| Word by Word Limited | Information & communication | 2017 |
| Yoda Bookkeeping Ltd | Professional & scientific | 2021 |
| Zak's Mystery Vapes & A-S Sattar C/Store Ltd | Wholesale & retail | 2025 |
| Zella Mechanical Services Limited | Manufacturing | 2020 |
| ZHR Foods Limited | Hospitality | 2025 |