| A.T.H. (Hydraulics) Limited | Manufacturing | 2001 |
| Amigos Blakeney Limited | Hospitality | 2024 |
| Axis Property Development Ltd | Construction | 2014 |
| Beddis Electrical Services Ltd | Construction | 2023 |
| Bilbo's Pet Spa Limited | Other services | 2016 |
| Blakeney Hill Growers Limited | Agriculture & fishing | 2010 |
| Cecil's Truck Styling Ltd | Wholesale & retail | 2024 |
| Clarke Broadcast Communications Limited | Information & communication | 2011 |
| Considera Limited | Professional & scientific | 2006 |
| Cotswold Contracting Services Limited | Construction | 2010 |
| Crux Elements Limited | Transport & logistics | 2020 |
| DD Capital Ltd | Transport & logistics | 2026 |
| Dean Mowers Limited | Manufacturing | 1998 |
| Dutchy MFG Limited | Wholesale & retail | 2020 |
| Forest Wills & Estate Planning Ltd | Professional & scientific | 2017 |
| Further Field Developments Limited | Construction | 2018 |
| Ginger Health Coaching Ltd | Health & social care | 2019 |
| Green Relocation Hub CIC | Construction | 2025 |
| Harker Clinic Ltd | Health & social care | 2025 |
| Hawthorn Retail Ltd | | 1964 |
| Hickmans Court Limited | Information & communication | 2025 |
| IN the Dance CIC | Arts & recreation | 2024 |
| J a & K J Davis Ltd | Construction | 2018 |
| J Double R Ltd | Professional & scientific | 2025 |
| J. Mcintosh Limited | Information & communication | 1998 |
| JMB & Company Trading Limited | Manufacturing | 2019 |
| KB Woodcrafts Limited | Manufacturing | 2012 |
| Ktrh Services Limited | Administrative & support | 2024 |
| KWO Manufacturing Solutions Ltd | Professional & scientific | 2023 |
| Land Rover Series One Club Limited | Other services | 1992 |
| Legal Care Limited | Professional & scientific | 1998 |
| Loiterpin Spaniels Ltd | Agriculture & fishing | 2022 |
| Mangu Consulting Limited | Financial services | 2014 |
| Mangu Wealth Management Limited | Financial services | 2021 |
| Old Manor Electric Company Limited | Energy | 2005 |
| PATHWAYS4FAMILIES Limited | Public administration | 2023 |
| Paul Cecil Limited | Transport & logistics | 2015 |
| Phoenix Packaging Ltd | Administrative & support | 2024 |
| Puppyccino Ltd | Hospitality | 2022 |
| Quorka Limited | Information & communication | 2025 |
| R50R53 Ltd | Other services | 2019 |
| Rmmpsco Limited | Professional & scientific | 2016 |
| Roads to Recovery CIC | Education | 2026 |
| Rombuk Limited | Professional & scientific | 2009 |
| Rosedean Properties Ltd | Real estate | 2018 |
| Severn Simulation Limited | Professional & scientific | 2025 |
| Sheridan Consulting Ltd | Professional & scientific | 2025 |
| Sheridan.Consult Limited | Health & social care | 2010 |
| Stirling Services (Forest of Dean) Limited | Administrative & support | 2013 |
| Tokr App Ltd | Information & communication | 2025 |
| Tony Livsey Limited | Information & communication | 2022 |
| Vintage Rocks Paint Ltd | Manufacturing | 2019 |
| W & N Adams Limited | Construction | 2008 |
| Wained Ltd | Extraterritorial | 2012 |
| Waite Holiday Cottages Limited | Hospitality | 2019 |
| Westway IT Ltd | Information & communication | 2018 |