| 2 Sisters Poultry Limited | Manufacturing | 2013 |
| 24/7 Lift Solutions Ltd | Manufacturing | 2015 |
| 3 Brown Bears Ltd | Professional & scientific | 2025 |
| A & P Grewar (Black Isle) Limited | Agriculture & fishing | 2009 |
| A and D Energy Co (Blairgowrie) Limited | Energy | 2016 |
| A F Scott and Company Limited | Hospitality | 1977 |
| A J Lyburn Limited | Agriculture & fishing | 1998 |
| A. Proctor Construction Limited | Manufacturing | 1997 |
| A.T.K. Holdings Limited | Financial services | 2017 |
| A1 Kilt Hire Edinburgh Ltd | Real estate | 2017 |
| A2E Limited | Administrative & support | 2002 |
| Abbey Cleaning Commercial Services Limited | Administrative & support | 2010 |
| Aberdeen Valet Services Limited | Other services | 2010 |
| About Beauty Ltd | Other services | 2026 |
| Abron Properties Ltd | Real estate | 2022 |
| Acam Property Limited | Real estate | 2024 |
| ACM Thermal Systems Ltd | Construction | 2026 |
| AD 3000 Building Developments Ltd | Construction | 2007 |
| Adam Smith Asset Management Limited | Professional & scientific | 2004 |
| Adam Smith Group Limited | Professional & scientific | 2004 |
| Adam Smith Property Management Limited | Professional & scientific | 2004 |
| ADAM2ADAM Garage Ltd | Wholesale & retail | 2022 |
| Adria House Limited | Hospitality | 2024 |
| Advance Project Planning Ltd | Information & communication | 2024 |
| Aesthetics AVE. Limited | Other services | 2024 |
| Aetos Research LLP | | 2016 |
| AGM Stone Ltd | Manufacturing | 2018 |
| Agra Drainage Limited | Extraterritorial | 2008 |
| Agrix Limited | Administrative & support | 2014 |
| AIM for Freedom Ltd | Hospitality | 2018 |
| Air Advantage Limited | Transport & logistics | 2011 |
| Airneis Ltd | Manufacturing | 2023 |
| Airth Electrical Ltd | Construction | 2020 |
| AJL Energy Limited | Real estate | 2025 |
| AL Elite Events Ltd | Hospitality | 2022 |
| Alan Milton Property Limited | Real estate | 2020 |
| Aldezan Limited | Construction | 2011 |
| Allcapscircus Limited | Arts & recreation | 2026 |
| Ally Scott Plumbing & Heating Ltd | Construction | 2021 |
| Always BE Limited | Wholesale & retail | 2026 |
| Alyth Auto Centre Ltd | Wholesale & retail | 2025 |
| Alyth Development Trust Limited | Administrative & support | 2015 |
| Alyth Garage Limited | Wholesale & retail | 2017 |
| Alyth Store Limited | Wholesale & retail | 2025 |
| Amplified Clothing Ltd | Wholesale & retail | 2024 |
| AMS Rigging and Lifting Services Ltd | Professional & scientific | 2017 |
| AMS Sports (Scotland) Limited | Wholesale & retail | 2018 |
| Anchor Bar Broughty Ferry Ltd | Hospitality | 2025 |
| Anna Duncan Butler Services Ltd | Hospitality | 2023 |
| AOA Surfacing Ltd | Construction | 2021 |
| Archid Architects Limited | Professional & scientific | 2009 |
| Ardblair Pest Control Limited | Administrative & support | 2021 |
| Ardblair Property Solutions Ltd | Administrative & support | 2023 |
| Ardblair Sports Importers Limited | Wholesale & retail | 1977 |
| Ardle Crichton Limited | Construction | 2018 |
| Ardle Property Ltd | Real estate | 2015 |
| Ardler Eggs Limited | Agriculture & fishing | 2026 |
| Armawrap Limited | Manufacturing | 2009 |
| Arooj Rehman Ltd | Wholesale & retail | 2025 |
| Artemis Alba Limited | Real estate | 2025 |
| Arthur Street News Ltd | Wholesale & retail | 2021 |
| Arthurstone Coffee Roasters Ltd | Manufacturing | 2023 |
| ASB Offshore Limited | Administrative & support | 2016 |
| Assured Roofing Systems Ltd | Construction | 2026 |
| Astra Aromatica Limited | Wholesale & retail | 2025 |
| Astragal Holdings Ltd | Real estate | 2023 |
| AT Pretium JET Charter Ltd | Transport & logistics | 2019 |
| Avionexus Ltd | Information & communication | 2024 |
| Axiom Specialists Ltd | Professional & scientific | 2012 |
| B&P Courier Services Ltd | Transport & logistics | 2024 |
| BAL Enterprises Limited | Hospitality | 2007 |
| Balbirnie Snawflake Ltd | Wholesale & retail | 2025 |
| Balnabroich Farms | | 1990 |
| Barrdrum Sales Limited | Agriculture & fishing | 2002 |
| Barry Wallace Joinery Ltd | Construction | 2025 |
| BDM Care Limited | Health & social care | 2012 |
| Beagan Limited | Real estate | 2023 |
| Beechtree Cottage Ltd | Hospitality | 2021 |
| Betula (Geotech) Ltd | Agriculture & fishing | 2015 |
| Bikepacking Scotland Ltd | Real estate | 2022 |
| Bingham Spence Limited | Professional & scientific | 2025 |
| Bioregioning Tayside Community Interest Company | Professional & scientific | 2022 |
| Bites and Beatz Limited | Hospitality | 2025 |
| Blackcraig Limited | Manufacturing | 2020 |
| Blaeberry Brae Woodland Ltd | Agriculture & fishing | 2025 |
| Blair Foods Ltd | Hospitality | 2021 |
| Blair SKI Hire & Active Breaks Ltd | Arts & recreation | 2011 |
| Blair Tandoori Ltd | Hospitality | 2024 |
| Blair Tech Repair Limited | Wholesale & retail | 2026 |
| Blairgowrie & Rattray Development Trust Limited | Other services | 2008 |
| Blairgowrie and East Perthshire Tourist Association Limited | Other services | 2011 |
| Blairgowrie Auto Services Ltd | Wholesale & retail | 2023 |
| Blairgowrie Autobody Ltd | Wholesale & retail | 2026 |
| Blairgowrie Community Project | Arts & recreation | 1998 |
| Blairgowrie Estate Limited | Real estate | 2013 |
| Blairgowrie Farm Shop LLP | | 2005 |
| Blairgowrie Garage Ltd | Wholesale & retail | 2023 |
| Blairgowrie New Tennis Club | Arts & recreation | 2011 |
| Blairgowrie Physiotherapy & Sports Injury Clinic Ltd. | Health & social care | 2014 |
| Blairgowrie Property Ltd | Real estate | 2026 |