| EMBROIDER8 Ltd | Wholesale & retail | 2018 |
| Emmchris 1 Limited | Real estate | 2020 |
| Emmchris Properties Limited | Real estate | 2020 |
| Employable Limited | Administrative & support | 2000 |
| Enchanted Events (North East) Ltd | Hospitality | 2024 |
| Endersby Group Limited | Financial services | 2019 |
| Endersby Property Group Ltd | Real estate | 2017 |
| Enginuity Digital Ltd | Information & communication | 2018 |
| Enterprise House Darlington Limited | Real estate | 2023 |
| Enterprise House Darlington Management Company Limited | Real estate | 2023 |
| Enzo and Luca Ltd | Real estate | 2019 |
| EOM Bars Ltd | Hospitality | 2026 |
| Epem Management Company Limited | Households | 2019 |
| Equilibrium Engineering (N/E) Limited | Professional & scientific | 2016 |
| ER Plumbing & Heating Ltd | Construction | 2025 |
| Errol & Temple Co. Ltd | Wholesale & retail | 2015 |
| ESF Access Ltd | Construction | 2023 |
| ESR Construction Safety Training Limited | Professional & scientific | 2017 |
| Essential Booth Ltd | Professional & scientific | 2019 |
| Essk Properties Ltd | Real estate | 2023 |
| Estatecodes Ltd | Professional & scientific | 2020 |
| Estiky Ltd | Professional & scientific | 2018 |
| Eston Property Investments Ltd | Real estate | 2016 |
| Ether Acquisitions Limited | Wholesale & retail | 2024 |
| Etherley Cricket Club Limited | Hospitality | 2012 |
| Etherley Meadows Management Company Limited | Households | 2022 |
| Etherley Moor Management Company Limited | Extraterritorial | 2014 |
| Etherley Properties Ltd | Real estate | 2021 |
| Euro Heat Exchangers Ltd | Manufacturing | 2024 |
| Euro Truck Bodies Limited | Manufacturing | 2003 |
| European Spraypainting Services Limited | Construction | 2015 |
| Evancliffe Ltd | Manufacturing | 2014 |
| Evans Bespoke Builds Limited | Construction | 2022 |
| Evenii Fencing & Landscaping Ltd | Administrative & support | 2025 |
| Evenii Group Limited | Financial services | 2023 |
| Evenii Limited | Construction | 2020 |
| Evenwood Powerlifting Gym Limited | Arts & recreation | 2020 |
| Evolve and Thrive Limited | Education | 2026 |
| Ewart Group Limited | Financial services | 2025 |
| EX626 Ltd | Administrative & support | 2018 |
| Excursions by Rail Limited | Administrative & support | 2021 |
| Exeltech Limited | Professional & scientific | 2012 |
| Explorers Daycare Limited | Health & social care | 2018 |
| Express Food Supplies Limited | Wholesale & retail | 2015 |
| F.E.R.N Outdoor Adventures C.I.C. | Arts & recreation | 2017 |
| F.M.K. Services Limited | Construction | 2007 |
| F.R.F. North East Limited | Construction | 2025 |
| Facilities Leadership UK Ltd | Education | 2023 |
| Fairfield Croft Management Company Limited | Households | 2019 |
| Fairhurst & SON Ltd | Construction | 2021 |
| Fairhurst Builders UK Ltd | Construction | 2025 |
| Faith Property Investments and Consultancy Services Limited | Real estate | 2014 |
| Fallows Park Management Company Limited | Households | 2016 |
| Fame Factory Spotlight Ltd | Education | 2008 |
| Farm Post Mortems Limited | Professional & scientific | 2013 |
| Farplace Trading Limited | Manufacturing | 2017 |
| Farsight Tastes Limited | Extraterritorial | 2015 |
| Fast FIX Interiors Limited | Construction | 2015 |
| Fcsl Compliance Limited | Construction | 2024 |
| Feather Hill Farm Limited | Real estate | 2019 |
| Fell View Enterprises Ltd | Wholesale & retail | 2025 |
| Fells Ecology Limited | Professional & scientific | 2022 |
| Fenwick & Dickinson Limited | Real estate | 2025 |
| FGS Consulting Limited | Professional & scientific | 2016 |
| Fifteas @ Stanhope Ltd | Hospitality | 2024 |
| Fifteas Ltd | Hospitality | 2023 |
| Finklegate Properties Limited | Real estate | 1984 |
| Fireproof Window Company Limited | Manufacturing | 2020 |
| First Electric Solutions Limited | Construction | 2020 |
| First React Engineering Ltd | Manufacturing | 2021 |
| First Time Groundworks Ltd | Construction | 2021 |
| Fishy FRY Ltd | Hospitality | 2025 |
| Flag Security Group Ltd | Administrative & support | 2020 |
| Flag Security Group Training Academy Ltd | Education | 2025 |
| Flames Pizzeria 4U Ltd | Hospitality | 2026 |
| Floored by LEE Bates Limited | Extraterritorial | 2013 |
| Flooring Superstore Limited | Wholesale & retail | 2013 |
| Floors Ahead Limited | Construction | 2018 |
| FLP Joinery Ltd | Construction | 2022 |
| Focus CUP Ltd | Arts & recreation | 2018 |
| Foodie Book Club CIC | Administrative & support | 2021 |
| Forensic Records Two Ltd | Information & communication | 2020 |
| Form Product Design Limited | Professional & scientific | 2008 |
| Foundry Hall Consulting Ltd | Administrative & support | 2021 |
| Foundry Hall Ltd | Financial services | 2022 |
| Fountain Design Limited | Manufacturing | 2002 |
| Four W Consulting Ltd | Professional & scientific | 2026 |
| Fpwc (Surveys)Ltd Ltd | Construction | 2024 |
| Fpwc Ltd | Construction | 2023 |
| Frank Hudson Transport Limited | Transport & logistics | 2000 |
| Franks Taverns Limited | Hospitality | 2024 |
| Fresh Solutions Cockfield Ltd | Administrative & support | 2024 |
| Friarside Consultancy Ltd | Professional & scientific | 2025 |
| Frosty's Ice Blasting Solutions Ltd | Administrative & support | 2025 |
| Fssg Ltd | Transport & logistics | 2025 |
| FT Property Solutions Ltd | Real estate | 2023 |
| Fuji Racing Ltd | Wholesale & retail | 2019 |
| Furnace Fitness Ltd | Arts & recreation | 2025 |
| Furness Property Solutions Limited | Energy | 2014 |
| Fury Events Activities Limited | Arts & recreation | 2012 |