| Eastick Homes Ltd | Real estate | 2024 |
| Eastlands Engineering NE Ltd | Professional & scientific | 2013 |
| Easy LET Properties (U.K.) Limited | Real estate | 2000 |
| Ebbie Engineering Ltd | Professional & scientific | 2022 |
| EBU Construction and Planning Ltd | Information & communication | 2020 |
| Eco Refurbs and Rentals Limited | Real estate | 2025 |
| Eden Instrumentation Ltd | Professional & scientific | 2025 |
| Eden Training Academy Limited | Education | 2016 |
| Edgefactor Ltd | Professional & scientific | 2021 |
| Education Enterprise Legacy Limited | Education | 2011 |
| Edward Smart & Sons Inspection Services Limited | Professional & scientific | 2011 |
| Effitech Limited | Professional & scientific | 2022 |
| EHO Management Ltd | Construction | 2025 |
| EJ PUB Company Ltd | Hospitality | 2026 |
| Ejoa Ltd | Health & social care | 2023 |
| Electric Ibiza Holdings (UK) Ltd | Arts & recreation | 2018 |
| Electronic Concept Designs Limited | Professional & scientific | 2023 |
| Elevate Aesthetics by Abby Ltd | Wholesale & retail | 2023 |
| Elhag-Medical Ltd | Health & social care | 2024 |
| Elite Home Improvements Teesside Limited | Construction | 2025 |
| Elite Joinery Solutions Ltd | Construction | 2021 |
| Elliott's Brew House Ltd | Hospitality | 2019 |
| Elliton Ltd | Construction | 2019 |
| Elmtree Medical Ltd | Health & social care | 2023 |
| Ember Property Ltd | Real estate | 2017 |
| Emco Site Services Ltd | Manufacturing | 2023 |
| EMD Hospitality Ltd | Hospitality | 2025 |
| Emdee Partnership LLP | | 2020 |
| Emerald Living Ltd | Hospitality | 2022 |
| Emerson Residential Properties Limited | Real estate | 1999 |
| Emmerson Engineering & Design Services Limited | Professional & scientific | 2008 |
| Enders Holdings LLP | | 2022 |
| Endofunctor Solutions Limited | Information & communication | 2023 |
| Endometriosis Care Clinic Northeast Ltd | Health & social care | 2025 |
| Energi Capital Ltd | Financial services | 2020 |
| Energy Contractor Hub Ltd | Energy | 2018 |
| Energy Costs Managed Limited | Wholesale & retail | 2021 |
| Engadine Family Partnership LLP | | 2021 |
| Engadine Homes Ltd | Real estate | 2021 |
| Engineer and Construct Ltd | Construction | 2025 |
| Enhanced Connections Psychology Limited | Other services | 2021 |
| Entity Properties LLP | | 2019 |
| Entropea Labs Limited | Professional & scientific | 2014 |
| Enzo's Pizzeria (Billingham) Ltd | Hospitality | 2024 |
| Erinscape Property Limited | Real estate | 2024 |
| ERO Pizza Ltd | Hospitality | 2025 |
| Esencial Consulting Ltd | Information & communication | 2019 |
| ESL Wrigley Offshore Limited | Professional & scientific | 2019 |
| ESP Professional Services Ltd | Education | 2010 |
| Essencias Holdings Ltd | Real estate | 2022 |
| Essencias Partners LLP | | 2022 |
| Etho`S (North East) Limited | Real estate | 2004 |
| Euro Contract Leasing Limited | Administrative & support | 1998 |
| Europa Management Limited | Health & social care | 1997 |
| Evans Family Group Holdings Ltd | Real estate | 2021 |
| Evans Family Partnership LLP | | 2021 |
| Everluckies Ltd | Real estate | 2025 |
| Eversdens Limited | Real estate | 2010 |
| Eversley Management Limited | Real estate | 2002 |
| EVH First Limited | Real estate | 2017 |
| Evolution Tech Services Ltd | Information & communication | 2013 |
| Excelletz LLP | | 2019 |
| Excelsior Global Investments Ltd | Real estate | 2015 |
| Exclusive Addresses Ltd | Households | 2016 |
| Exclusive Short Stays Estates Limited | Real estate | 2020 |
| Exclusive Short Stays Ltd | Real estate | 2019 |
| Expo Market Limited | Information & communication | 2022 |
| EZ Industries Ltd | Information & communication | 2024 |
| F1RST Stop Ltd | Professional & scientific | 2019 |
| FA & JF Co Ltd | Administrative & support | 2013 |
| Fahmzz Ltd | Wholesale & retail | 2023 |
| Fairoak Corporation Limited | Construction | 1994 |
| Fairoak Estates Limited | Real estate | 2019 |
| Fairoak Partnership LLP | | 2023 |
| Fairoak Property Group Ltd | Financial services | 2020 |
| Fairway Contracts Limited | Construction | 2011 |
| Fallon Family Partnership LLP | | 2017 |
| Family Shopper (Middlesbrough) Limited | Wholesale & retail | 2021 |
| Faroughian Family Holdings Ltd | Real estate | 2019 |
| Faroughian Family Partnership LLP | | 2019 |
| Faroughian Trading Ltd | Real estate | 2025 |
| Fastforex Limited | Financial services | 2025 |
| Fastidious LLP | | 2022 |
| Fbtc Limited | Professional & scientific | 2011 |
| Fellowship Homes Partnership LLP | | 2021 |
| Fens Property Ltd | Real estate | 2023 |
| Fides Accounting Services Ltd | Professional & scientific | 2021 |
| Fields & Co Property Services Ltd | Education | 2026 |
| FIG Tree Illustration Co Limited | Wholesale & retail | 2022 |
| Figham Gate RMC Limited | Households | 2026 |
| FIN & Tech Business Developments Ltd | Professional & scientific | 2021 |
| Findmotion Ltd | Information & communication | 2018 |
| Firth Vickers Drilling Ltd | Manufacturing | 2024 |
| Fitzroy of London Limited | Manufacturing | 2011 |
| Fleck Consulting and Business Services Limited | Professional & scientific | 2021 |
| Fleetwash North East Limited | Wholesale & retail | 2016 |
| Fleming Group & Co Ltd | Other services | 2022 |
| Fleur Hensam Ltd | Professional & scientific | 2026 |
| Floor LAY Ltd | Wholesale & retail | 2015 |
| Floortech Systems Ltd | Wholesale & retail | 2013 |