| Diamond Blue Properties Ltd | Real estate | 2020 |
| Digital Reliance Limited | Other services | 2021 |
| Dignity LC Services Ltd | Manufacturing | 2014 |
| Dillz Eatery Ltd | Wholesale & retail | 2024 |
| Direxions Limited | Professional & scientific | 2004 |
| Discount Healthcare Ltd | Wholesale & retail | 2017 |
| DJM Specialist Limited | Information & communication | 2016 |
| DMP Carpentry and Building Ltd | Construction | 2024 |
| DMP Hygiene Services Ltd | Health & social care | 2024 |
| DMW Retail Solutions Limited | Professional & scientific | 2023 |
| DNM Property Developments Limited | Construction | 2004 |
| DNS Performance Ltd | Wholesale & retail | 2010 |
| Docking Management Company Limited | Extraterritorial | 2014 |
| Dog in the Building Ltd | Arts & recreation | 2025 |
| Dolan Homes Ltd | Real estate | 2020 |
| Domestic & Commercial (Contracts) Ltd | Administrative & support | 2018 |
| Dopamine Ropes Ltd | Wholesale & retail | 2025 |
| Dopamyne Ltd | Wholesale & retail | 2023 |
| Dope Ropes Ltd | Wholesale & retail | 2015 |
| Dormer Property Services Limited | Construction | 2024 |
| Dough Bros Limited | Wholesale & retail | 2022 |
| DR Jerome Ltd | Health & social care | 2011 |
| DR Laura Keyes & Associates Ltd | Health & social care | 2018 |
| Dr.At Ltd | Health & social care | 2026 |
| Drama Queen Productions Ltd | Information & communication | 2019 |
| DRD Build Ltd | Construction | 2025 |
| Dreadrock Ltd | Manufacturing | 2019 |
| Driftwood Joinery Limited | Manufacturing | 2007 |
| Drink Tasting Room Limited | Wholesale & retail | 2020 |
| Driveway Deals Limited | Wholesale & retail | 2025 |
| DS Design and Build Limited | Construction | 2014 |
| DSL Clinical Psychology Services Limited | Health & social care | 2021 |
| Dtex Technologies Ltd | Information & communication | 2022 |
| Dunton Battery Farm LLP | | 2025 |
| DUO Plumbing and Carpentry Ltd | Construction | 2026 |
| DWB Carpentry Limited | Manufacturing | 2025 |
| Dwinc Ltd | Information & communication | 2014 |
| Dynamiq Applications Limited | Wholesale & retail | 2011 |
| E-Man Limited | Professional & scientific | 2000 |
| EAD Services Ltd | Professional & scientific | 2023 |
| Eagle RAY Logistics Ltd | Transport & logistics | 2025 |
| Eary Consulting Limited | Information & communication | 2021 |
| Easie Tech Ltd | Information & communication | 2016 |
| Ebanehita Limited | Health & social care | 2014 |
| Echo Talent Ltd | Administrative & support | 2023 |
| ECI Solutions Limited | Professional & scientific | 2012 |
| ECN Talent Ltd | Arts & recreation | 2024 |
| Eco Plumbing Services Limited | Construction | 2008 |
| Eco-Property Group Limited | Construction | 2017 |
| Ecomm - Voice Network Solutions Ltd | Information & communication | 2022 |
| Ecoplaster Ltd | Construction | 2018 |
| Edwards Installations Limited | Manufacturing | 2011 |
| Edworth Barns Management Limited | Households | 1996 |
| Eggline Properties Ltd | Real estate | 2022 |
| EL Recruitment Ltd | Administrative & support | 2020 |
| Electronic Consultancy Services Ltd | Wholesale & retail | 2020 |
| Element Collective Ltd | Other services | 2020 |
| Elisam 01 Ltd | Wholesale & retail | 2023 |
| Elliott Bay Towers Limited | Arts & recreation | 2023 |
| ELM View Ventures Ltd | Households | 2024 |
| Eltron Europe Ltd | Manufacturing | 2004 |
| Eltron UK PCB Limited | Manufacturing | 2021 |
| EMC Solutions Limited | Manufacturing | 2015 |
| Emily Thornton School of Dance Limited | Arts & recreation | 2016 |
| Emma Phillips Consultancy Ltd | Professional & scientific | 2018 |
| Emms U.K. Limited | | 1982 |
| Emotivae Limited | Professional & scientific | 2025 |
| Empowering Care Services Ltd. | Health & social care | 2019 |
| Endelevu Ltd | Professional & scientific | 2023 |
| Endoline Automation Limited | Manufacturing | 2014 |
| Endoline Machinery Limited | Manufacturing | 1981 |
| Endoline Robotics Ltd | Manufacturing | 2019 |
| Enotec UK Ltd. | Wholesale & retail | 1990 |
| ENZ UK Properties Limited | Real estate | 2019 |
| Epicure Creative Web Development Ltd | Information & communication | 2020 |
| Epponnee RAE Limited | Arts & recreation | 2004 |
| Epsilon Estates Ltd | Real estate | 2015 |
| Esavian Limited | Manufacturing | 1989 |
| Eska U.K. Ltd | Arts & recreation | 2024 |
| Evolution Home Improvements Ltd | Construction | 2023 |
| Excalibur Relocation Consultancy Ltd | Transport & logistics | 2022 |
| Excelsior Mart Ltd | Wholesale & retail | 2024 |
| EYE Consultants International Ltd | Arts & recreation | 2026 |
| F&L Real Estate Ltd | Real estate | 2026 |
| F.G. and E.A. Ball Limited | Transport & logistics | 2013 |
| Factory Grips Ltd | Information & communication | 2014 |
| Fair Trans Ltd | Transport & logistics | 2019 |
| FAR & Ride Limited | Administrative & support | 2005 |
| Faversham Studio Ltd | Professional & scientific | 2020 |
| Feneley Acquisitions Ltd | Real estate | 2020 |
| Finance Giant Ltd | Financial services | 2015 |
| Finishing Trade Limited | Construction | 2014 |
| Finlo Solutions Ltd | Professional & scientific | 2018 |
| Finlogics Limited | Real estate | 2005 |
| Fintnana Consult Ltd | Professional & scientific | 2024 |
| Firearms Dealer UK Limited | Manufacturing | 2023 |
| Fireaway Biggleswade Kp&Sons Limited | Hospitality | 2022 |
| First Call Partners Limited | Professional & scientific | 2017 |
| First Fundraising Ltd | Information & communication | 2024 |
| Fisher Contracting Limited | Professional & scientific | 2015 |