| Rivertree Consulting Limited | Professional & scientific | 2006 |
| RMC Repairs Limited | Other services | 2021 |
| Rmml Limited | Real estate | 2000 |
| ROB ROY Park Homes Ltd | Hospitality | 2019 |
| Robert B Drife (Farm Account) | | 1987 |
| Roberton Vetecho Mobile Services Ltd | Professional & scientific | 2025 |
| Robertson and Macintyre Ltd | Real estate | 2025 |
| Rocstel Ltd | Professional & scientific | 2026 |
| Roxon International L.P. | | 2012 |
| Rural Matters LLP | | 2010 |
| RW Brown Limited | Wholesale & retail | 2009 |
| Saintbor Impex LP | | 2013 |
| Saltire Driveways & Patios Ltd | Administrative & support | 2023 |
| SAS Steering Alignment Services Ltd | Wholesale & retail | 2025 |
| Scene NOT Herd Ltd | Arts & recreation | 2013 |
| Scott Vehicle Repairs Limited | Wholesale & retail | 2013 |
| Sheildall Garden Trust Ltd | Agriculture & fishing | 2026 |
| SKK Productions Limited | Information & communication | 2009 |
| Smail & Ewart Ltd | Professional & scientific | 2019 |
| Solento Business LP | | 2013 |
| Soul Remedy Community Interest Company | Information & communication | 2023 |
| Spectrum Impulse Ltd | Information & communication | 2016 |
| Spittal Farm Partners LLP | | 2018 |
| Squirrel OPS Ltd | Professional & scientific | 2026 |
| Stellar Armata Ltd | Hospitality | 2019 |
| Stotts Motor Garage Ltd | Wholesale & retail | 2025 |
| Strathclyde Cash Registers Limited | Wholesale & retail | 1999 |
| Sunny Side Eggs Limited | Agriculture & fishing | 2023 |
| Sutton West Limited | Hospitality | 2024 |
| T. & J. Linton Limited | Agriculture & fishing | 2006 |
| Table 33 Limited | Construction | 2017 |
| Tairis Management LP | | 2015 |
| TAJ Biggar Ltd | Hospitality | 2022 |
| Tavert Business LP | | 2014 |
| Tawse ITM Limited | Construction | 2013 |
| Terexim LP | | 2017 |
| Termex UK Ltd | Wholesale & retail | 2025 |
| Terrateres (Scotland) Limited | Financial services | 2015 |
| THE Biggar Albion Foundation Limited | Arts & recreation | 2001 |
| THE Biggar GIN Company Limited | Wholesale & retail | 2016 |
| THE Hideaway at Carmichael Estate Ltd | Hospitality | 2025 |
| THE Historical Herbalists CIC | Education | 2025 |
| THE Kirkstyle Ltd | Hospitality | 2026 |
| THE Little West END Theatre CIC | Arts & recreation | 2024 |
| THE Scottish Carriage Driving Association Limited | Arts & recreation | 1979 |
| THE Tweed Pantry Ltd | Hospitality | 2025 |
| Third Places Studio Limited | Professional & scientific | 2016 |
| Thistle Fern Media Ltd | Information & communication | 2018 |
| Thomson Computer Systems Limited | Other services | 2010 |
| Tinto Drinks Limited | Wholesale & retail | 2024 |
| Tinto Loch Lodge Limited | Hospitality | 2023 |
| TOM Bone Plant Services Ltd | Manufacturing | 2022 |
| Tondray Impex LP | | 2014 |
| Tonlad Impex LP | | 2014 |
| Tordel Impex LP | | 2015 |
| Toria LP | | 2015 |
| Torquecode Ltd | Information & communication | 2013 |
| Trailer Services Scotland Ltd | Wholesale & retail | 2024 |
| Treesurv Limited | Agriculture & fishing | 2015 |
| Trinity Heart Limited | Professional & scientific | 2016 |
| Tulip Thistle Medical Services Ltd | Health & social care | 2017 |
| Turbochem LP | | 2016 |
| Turdel LP | | 2016 |
| Tweedsmuir Trading Group Ltd | Other services | 2022 |
| Twentyeleven Properties Ltd | Real estate | 2024 |
| Two Rivers Veterinary Practice Limited | Professional & scientific | 2013 |
| Verdia Commerce LP | | 2014 |
| Vertus Solutions Ltd | Professional & scientific | 2023 |
| Vespol Trade LP | | 2015 |
| Veted Specialists Ltd | Professional & scientific | 2019 |
| Virtual Manufacturing UK Limited | Professional & scientific | 2022 |
| W J W Joinery Ltd | Construction | 2014 |
| W K Harvey (Baitlaw) & Co | | 1985 |
| Wandel Holdings Limited | Financial services | 2024 |
| Waterend Ltd | Professional & scientific | 2024 |
| Wellsgray Consulting Ltd | Information & communication | 2011 |
| Werland Alliance LP | | 2013 |
| Wheelie Fresh and Clean Limited | Administrative & support | 2021 |
| White Agri Tuning Ltd | Manufacturing | 2021 |
| White's Motor Engineers Limited | Wholesale & retail | 2003 |
| Wigram Consulting Limited | Professional & scientific | 2011 |
| William Johnson & SON (Wishaw) Limited | Construction | 1942 |
| Winlocke Ltd | Hospitality | 2024 |
| Wiston Lodge | Education | 2007 |
| Wood for the Trees Limited | Hospitality | 2017 |
| Wright & Co Design and Installation Limited | Construction | 2014 |
| XDR Engineering Ltd | Manufacturing | 2025 |
| Yoke Business Support Ltd | Administrative & support | 2025 |