| 1531 Retreats Ltd | Hospitality | 2024 |
| A 1 Grab Timber Ltd | Agriculture & fishing | 2025 |
| A Fyfe Rail Operations Consulting Ltd | Professional & scientific | 2015 |
| A J I Creative Ltd. | Information & communication | 2021 |
| A73 Recovery Ltd | Transport & logistics | 2023 |
| Aacec Ltd | Real estate | 2019 |
| Acorn to Oak Property Ventures Ltd | Real estate | 2025 |
| AD HOP Limited | Professional & scientific | 2024 |
| Adiantum LP | | 2015 |
| Agaro LP | | 2016 |
| Air Pressure Testing Scotland Ltd | Construction | 2017 |
| Air Testing Scotland Limited | Other services | 2011 |
| Alba Blinds Limited | Wholesale & retail | 2002 |
| Aldesta LP | | 2014 |
| Alec Pearson Coaching Ltd | Professional & scientific | 2022 |
| Amberdark Ltd | Professional & scientific | 2015 |
| Amethyst Technologies Limited | Information & communication | 2011 |
| Anderson Ballantine Limited | Professional & scientific | 2008 |
| Andrew Cowan Joinery Services Limited | Other services | 2007 |
| APD Real Estate Limited | Real estate | 2026 |
| APL Fire Suppression Limited | Administrative & support | 1996 |
| APR Properties Limited | Real estate | 1945 |
| Ardis Ventures LP | | 2013 |
| ARK Hill Wind Farm 2 Limited | Energy | 2021 |
| Armac Services Limited | Financial services | 2021 |
| Armac Vets Limited | Professional & scientific | 2012 |
| ARW Barrie Ltd | Transport & logistics | 2026 |
| ARW Barrie Properties Limited | Real estate | 2021 |
| Asia Transnational L.P. | | 2015 |
| Atlas Winch & Hoist Services (Southern) Ltd. | Administrative & support | 2001 |
| Atlas Winch & Hoist Services Ltd. | Wholesale & retail | 1998 |
| Atreus Events Ltd | Information & communication | 2019 |
| AV Tech Hire Ltd | Information & communication | 2019 |
| B2GS Properties Ltd | Real estate | 2024 |
| Balnagown Ltd | Real estate | 2025 |
| Belford Research Limited | Professional & scientific | 2008 |
| Bellscraig Limited | Financial services | 2025 |
| Belwor Impex LP | | 2014 |
| Bess Infrastructure Ltd | Real estate | 2023 |
| Bess Wind Limited | Agriculture & fishing | 2013 |
| BF Property Ltd | Real estate | 2019 |
| Biggar & District Community Heritage Limited | Arts & recreation | 2005 |
| Biggar Biomass Ltd | Extraterritorial | 2013 |
| Biggar Braai Ltd | Wholesale & retail | 2024 |
| Biggar Cars Limited | Other services | 2011 |
| Biggar Community Golf & Caravan Park Limited | Hospitality | 2025 |
| Biggar Corn Exchange Ltd | Education | 2009 |
| Biggar Estate Ltd | Real estate | 2024 |
| Biggar Eyecare Limited | Wholesale & retail | 2022 |
| Biggar Food Growing Group Community Interest Company | Agriculture & fishing | 2021 |
| Biggar Investments Ltd | Real estate | 2021 |
| Biggar Museum Trust | Arts & recreation | 2011 |
| Biggar Piezo Technology Ltd | Wholesale & retail | 2011 |
| Biggarshiels Carriages Ltd | Agriculture & fishing | 2006 |
| Blazin Fiddles Limited | Arts & recreation | 2006 |
| Bombcrater Design Limited | Professional & scientific | 2016 |
| Border Mass Spec Ltd | Professional & scientific | 2025 |
| Border Tablet Ltd | Manufacturing | 2017 |
| Borders Meadows Trust | Professional & scientific | 2022 |
| Bothy Garden Rooms Ltd | Construction | 2017 |
| Bowswell Ltd | Other services | 2022 |
| Brave New Europe Ltd | Information & communication | 2024 |
| Braw Clan Limited | Arts & recreation | 2021 |
| Breaching Business Limited | Professional & scientific | 2016 |
| BRL Laser Damage Ltd | Professional & scientific | 2024 |
| Broadfield Farming Company Limited | Agriculture & fishing | 1991 |
| Broughton Ales Limited | Manufacturing | 1995 |
| Broughton Community PUB CIC | Hospitality | 2025 |
| Broughtonknowe Community Woodland Ltd | Agriculture & fishing | 2024 |
| C.A.J Property Investments Ltd | Other services | 2003 |
| C4U Limited | Information & communication | 2020 |
| Cairngryffe Foods Ltd | Wholesale & retail | 2024 |
| Cairngryffe Gundogs Ltd | Agriculture & fishing | 2019 |
| Caithness Estates Limited | Construction | 2022 |
| Caledonia Digital Print & Design Ltd | Information & communication | 2020 |
| Campbell Court Limited | Real estate | 2023 |
| Campbells Fudge Limited | Manufacturing | 2011 |
| Campfire Ltd | Hospitality | 2023 |
| Candybank Property Ltd | Real estate | 1986 |
| Carmichael Heritage and Leisure Limited | Hospitality | 1989 |
| Cattletech Scotland Ltd | Agriculture & fishing | 2025 |
| CCM Laboratories Ltd | Health & social care | 2018 |
| Cdev Properties Ltd | Households | 2022 |
| Cdmb Ltd | Real estate | 2021 |
| CH Forestry Consultancy Ltd | Agriculture & fishing | 2025 |
| Change Guide Consultancy Ltd. | Information & communication | 2019 |
| Changes Associates Limited | Wholesale & retail | 2017 |
| Charles Coubrough and Company Limited | Agriculture & fishing | 1956 |
| Cherry House 118 Ltd | Hospitality | 2021 |
| Citadel IT Consultancy Limited | Information & communication | 2011 |
| Citadelet Limited | Real estate | 2023 |
| City Decorators (Scotland) Ltd | Construction | 2025 |
| CK Chips Ltd | Construction | 2022 |
| Clark & Thomson Service Group Limited | Financial services | 2023 |
| Closbel LP | | 2016 |
| Clydesdale Technical Services Limited | Wholesale & retail | 2011 |
| Coachwise Ltd | Professional & scientific | 2021 |
| Cochon Plastique Limited | Information & communication | 2022 |
| Colourful Pricks Limited | Other services | 2022 |
| Common Weal Limited | Professional & scientific | 2014 |