| A & E Developments Limited | Wholesale & retail | 2006 |
| A.J.E. Calder Limited | Agriculture & fishing | 1956 |
| A.M. & a. Calder Farms Limited | Agriculture & fishing | 1957 |
| Aikyside Developments Limited | Construction | 2007 |
| Alan Thomson Fencing Ltd | Construction | 2007 |
| Alba Country Foods Limited | Transport & logistics | 1994 |
| Aldencraw Ltd | Hospitality | 2025 |
| Alemill Farm Partnership | | 1999 |
| Amco Capital Limited | Professional & scientific | 1990 |
| Andrew Cowan Motorsports Limited | Extraterritorial | 2004 |
| Andrew Jack & Co (Cranshaws) | | 2000 |
| Ayton Castle Farms Partnership | | 1995 |
| BHA Enterprise Ltd | Energy | 2002 |
| Billy Cowe (Commercials) Ltd | Wholesale & retail | 2013 |
| Carlsden Credit Management | | 1999 |
| Chirnside Growers Limited | Agriculture & fishing | 2011 |
| Craig Dewar (Plumbing and Heating) Limited | Construction | 2003 |
| Dalharco Ltd | Construction | 2007 |
| Darnchester Farming Holdings Limited | Financial services | 2023 |
| Darnchester Farming Limited | Agriculture & fishing | 2023 |
| Dean Wilson Plumbing & Heating Limited | Construction | 2007 |
| Dowries Farm | | 1987 |
| Drysdale Storage Limited | Transport & logistics | 2006 |
| Ebba Services Limited | Agriculture & fishing | 1996 |
| Ellemford Farms | | 1990 |
| Eyemouth Freezers Limited | Manufacturing | 1999 |
| Farne Salmon & Trout Limited | Manufacturing | 1981 |
| Fernyrig Farming Partnership | | 1987 |
| Firm of Milldown Farm | | 1999 |
| Fleming Farmers | | 2002 |
| Fleming Homes Construction Limited | Construction | 2016 |
| Fogo Fields | | 1989 |
| Gartmore Farms Limited | Agriculture & fishing | 1981 |
| Garvey Lauderhaugh & Company | | 2000 |
| George Mcdougal (Bassendean) Limited | Agriculture & fishing | 1963 |
| Golden Bannock Ltd. | Other services | 2017 |
| Greenvale Growing Ltd. | Agriculture & fishing | 2004 |
| H.G. Fleming | | 2000 |
| Hazel's Footprints Trust | Other services | 2004 |
| J. & G. Campbell Limited | Administrative & support | 1953 |
| J. & J. ORR Limited | Agriculture & fishing | 1956 |
| J. & J. Robertson | | 1989 |
| J.J. Patterson (Glengelt) | | 1996 |
| J.M. Controls and Engineering Limited | Professional & scientific | 2022 |
| J.M. Menzies L.S. Farm | | 1989 |
| Jack KAY & Sons | | 1993 |
| James Pate & Partners (Craigsford Mains) | | 1997 |
| JC & KC Constable Ltd | Agriculture & fishing | 2003 |
| JED Training Solutions Ltd | Education | 2012 |
| John Wilson & Sons (Farmers) Limited | Agriculture & fishing | 1993 |
| Kays and Biddulph | | 1991 |
| Kezie Leather Limited | Manufacturing | 1996 |
| Kezie Limited | Manufacturing | 1994 |
| KMH Contracts Limited | Other services | 2017 |
| Letham Farming Limited | Agriculture & fishing | 2004 |
| Light Kits Limited | Manufacturing | 2001 |
| Little Sweep Ltd. | Other services | 2024 |
| Littledean Farming Partnership | | 1999 |
| Lylestane Farm | | 1994 |
| M.J. Mcgregor & Co. | | 1989 |
| Macfarlane Farms Limited | Agriculture & fishing | 1997 |
| Marchmont Nurseries | | 1994 |
| Marigold Farming Company Limited | Agriculture & fishing | 1962 |
| Mcgregor Farms (Coldstream) Limited | Agriculture & fishing | 1976 |
| Meadowhead Limited | Hospitality | 1974 |
| Merryhall Farming Company Limited | Extraterritorial | 2018 |
| Messrs Peter Calder & Co | | 2000 |
| Modiolus Consulting Limited | Professional & scientific | 2016 |
| Mountfair Farming Limited | Agriculture & fishing | 2003 |
| MPS (Europe) Limited | Construction | 2002 |
| Muirton Farming Company | | 1987 |
| Nether Huntlywood Farmers | | 1999 |
| Old Cambus East Mains Farm | | 2000 |
| Old Cambus West Mains Farm | | 2001 |
| Peter Quayle & Co | | 1973 |
| Pittencrieff (Kinneddar) LLP | | 2006 |
| Putton Mill Golf Driving Range Limited | Arts & recreation | 2002 |
| R R Tweedie & Co | | 1985 |
| Rhymer Construction Limited | Construction | 2007 |
| Richard Amos Ltd. | Professional & scientific | 2002 |
| Rigfoot Farms | | 1990 |
| RM and JF Seed Ltd | Agriculture & fishing | 1980 |
| Robert Forrest Limited | Agriculture & fishing | 1978 |
| S. Hardy Limited | Agriculture & fishing | 1961 |
| Shirley Macgregor | | 1989 |
| Simprim Farms Ltd | Agriculture & fishing | 1957 |
| Sisterpath Farm | | 1990 |
| Springfield Farmers | | 1992 |
| Steel Space Casing Drilling Ltd. | Other services | 2018 |
| Stewart and Aitchison | | 1987 |
| T. Fleming Homes Limited | Manufacturing | 1986 |
| THE Highway Day Nursery | Education | 2003 |
| THE Littledean Farmcare Partnership | | 2002 |
| THE Mcgonigle Group Ltd | Wholesale & retail | 2020 |
| THE Rawburn Farm Partnership | | 1993 |
| THE Redhall Farm Partnership | | 1994 |
| THE Sustainable Beef Co. Ltd | Manufacturing | 2019 |
| Thorburn Holdings Limited | Other services | 2021 |
| Thos a Shaw | Hospitality | 1988 |
| Universal Fibre Optics Limited | Manufacturing | 1999 |