| SG Matrix Limited | Professional & scientific | 2004 |
| Sherylelaina Ltd | Health & social care | 2024 |
| Shidlaw Farming Limited | Agriculture & fishing | 2012 |
| Shiny Hand Car Wash & Valeting Ltd | Administrative & support | 2026 |
| Shoreswood Ltd | Administrative & support | 2004 |
| Simon Ainscough Farms Limited | Agriculture & fishing | 1998 |
| Single Planet Solutions Ltd | Professional & scientific | 2012 |
| Sixty SIX Developments Ltd | Construction | 2025 |
| Sky High Camera Limited | Professional & scientific | 2002 |
| Smith and Garratt Ltd | Extraterritorial | 2014 |
| Snipe Moss Ltd | Real estate | 2006 |
| Software Consultants (Tetbury) Limited | Information & communication | 2009 |
| Southfork Properties Ltd | Real estate | 2022 |
| Sparwindows Ltd. | Wholesale & retail | 2016 |
| Spital Mains Farm Ltd | Hospitality | 2019 |
| ST Aidans Management Company Limited | Households | 2019 |
| ST. Cuthberts Farmhouse Limited | Hospitality | 2007 |
| Stafford Charles Limited | Real estate | 2010 |
| Stagsbrook Game Ltd | Agriculture & fishing | 2022 |
| Starrett Precision Optical Limited | Manufacturing | 1990 |
| Starscape Star Ceilings Ltd | Manufacturing | 2014 |
| Step 128 Management Consulting Limited | Professional & scientific | 2026 |
| Steve Collin Ltd | Real estate | 2023 |
| Steven Monks Electrical Contractors Ltd | Construction | 2008 |
| Steven Robertson RA Ltd | Construction | 2024 |
| STM (Edinburgh) Care Limited | Health & social care | 2024 |
| Sub-Com (Subsea Commissioning) Limited | Professional & scientific | 1994 |
| Susan Ryder Limited | Arts & recreation | 2002 |
| Sussex Technology Limited | Information & communication | 2013 |
| SWG Killen Limited | Wholesale & retail | 2024 |
| T F Fitness Ltd | Arts & recreation | 2022 |
| T H Nixon & Sons Limited | Wholesale & retail | 2021 |
| T.S.A Inspection Services Limited | Professional & scientific | 2018 |
| Tailors Direct Ltd | Wholesale & retail | 2002 |
| Tappizza Limited | Hospitality | 2021 |
| TGR (Import & Export) UK Limited | Wholesale & retail | 2014 |
| TGR Access Ltd | Construction | 2025 |
| That POP Culture GUY Ltd | Wholesale & retail | 2025 |
| THE Anchor Practice Limited | Health & social care | 2019 |
| THE Book Loft Ltd | Wholesale & retail | 2025 |
| THE Courthouse Wallace Green Management Company Limited | Households | 2021 |
| THE Cross Inn Limited | Hospitality | 2015 |
| THE Lawn Mower Centre (Duns) Limited | Manufacturing | 2016 |
| THE Limeworks Ltd | Real estate | 2020 |
| THE Maltings (Berwick) Trust | Arts & recreation | 1988 |
| THE Mule on Rouge Ltd | Hospitality | 2017 |
| THE Old School House (Mount Road) Limited | Households | 2004 |
| THE Peacock Office Limited | Professional & scientific | 2011 |
| THE Percy Hunt Ltd | Administrative & support | 2004 |
| THE Retreat Advanced Aesthetics Limited | Other services | 2021 |
| THE Straw Yard C.I.C. | Health & social care | 2025 |
| THE Untangled Knot Limited | Information & communication | 2026 |
| THE Wilson's Tales Project | Information & communication | 2013 |
| Thomas Evans Properties Limited | Real estate | 2005 |
| Thomas Sherriff and Company Limited | Wholesale & retail | 1967 |
| Thorburn Group (Berwick) Limited | Construction | 2006 |
| Three Quay Walls Management Company Limited | Households | 1986 |
| Thrive Consulting Services Ltd | Professional & scientific | 2020 |
| Tiao Jewellery Ltd | Wholesale & retail | 2025 |
| Tiger Building Products Ltd | Wholesale & retail | 2014 |
| Tillmouth & Tweed Salmon Fishings LLP | | 2006 |
| Toddy Knowes Limited | Agriculture & fishing | 2023 |
| Tortive Theatre Ltd | Information & communication | 2019 |
| Tower Martin Contractors Limited | Administrative & support | 2007 |
| Town Walls Nursery Ltd | Education | 2022 |
| Trading Products Limited | Water & waste | 2016 |
| Traffic Safety Personnel Limited | Other services | 2003 |
| Trafficlenz UK Limited | Information & communication | 2020 |
| Travelled Music Ltd | Administrative & support | 2026 |
| Treespace Ltd | Agriculture & fishing | 2006 |
| Trinity Fencing & Decking Ltd | Construction | 2023 |
| TRS Ribbons Ltd | Manufacturing | 2014 |
| Turkleton Properties Ltd | Real estate | 2026 |
| Tweed Competitions Ltd | Extraterritorial | 2026 |
| Tweed Gallery Limited | Arts & recreation | 2022 |
| Tweed Mortgage Hub Ltd | Financial services | 2023 |
| Tweedmouth House Care Limited | Health & social care | 2002 |
| Tweedside Design Co. Ltd | Wholesale & retail | 2020 |
| U & J Concepts Ltd | Hospitality | 2013 |
| UBI One Solutions Ltd | Information & communication | 2019 |
| UBS Contract Management Limited | Construction | 2017 |
| Uddinggate Limited | Real estate | 2016 |
| Ultramech North East Ltd | Construction | 2020 |
| Unity Fitness Berwick Ltd | Arts & recreation | 2020 |
| Unleashed Dog Parks Ltd | Arts & recreation | 2012 |
| Userproof Ltd | Information & communication | 2014 |
| Vangard Holdings Ltd | Real estate | 2025 |
| Vault 7 Ltd | Information & communication | 2014 |
| Vekta Group Limited | Energy | 2017 |
| Victoria Tweed Limited | Hospitality | 2023 |
| Viewpoint Management Ltd | Real estate | 2009 |
| Visions Plus Limited | Other services | 2025 |
| VK Recruitment Limited | Professional & scientific | 2007 |
| W & B Park Limited | Administrative & support | 1978 |
| W.A. Riddell Transport Limited | Transport & logistics | 2016 |
| Waren Mill Management Company Limited | Real estate | 2001 |
| Warenton Farm Buildings (Management) Limited | Households | 2004 |
| Waterview Properties Limited | Professional & scientific | 1983 |
| Wayduke Limited | Wholesale & retail | 1985 |
| WB Managing Consultants Ltd | Professional & scientific | 2015 |