| Flannigan & Border Skip Hire Area 4 Ltd | Water & waste | 2017 |
| Flannigan & Border Skip Hire Area 6 Ltd | Water & waste | 2021 |
| Flavius Properties Limited | Hospitality | 2016 |
| Fleming Homes (England) Ltd | Construction | 2014 |
| FM Talent Management Consulting Ltd | Professional & scientific | 2012 |
| Fordhill Services Ltd | Professional & scientific | 2025 |
| Forthview Properties Limited | Real estate | 2017 |
| Frontier Communications Limited | Administrative & support | 2009 |
| Fusion Associates Limited | Wholesale & retail | 2001 |
| G & M Nisbet Haulage Ltd | Transport & logistics | 2016 |
| G J Riddell Property Ltd | Real estate | 2024 |
| G N Stores Limited | Wholesale & retail | 2020 |
| G. C. Grieve Limited | Wholesale & retail | 2000 |
| Galagate Developments Limited | Construction | 2008 |
| Gambettibarre UK Limited | Real estate | 1998 |
| Gateway Finance Limited | Professional & scientific | 1957 |
| Gawain Hewitt Limited | Arts & recreation | 2015 |
| Gclc Ltd | Wholesale & retail | 2026 |
| Geo-Min Ltd | Construction | 2026 |
| Gertrude Properties Limited | Administrative & support | 2002 |
| Ghrc Consulting Limited | Information & communication | 2018 |
| Gilmerton Care Limited | Health & social care | 2024 |
| GMO Consultancy Ltd | Administrative & support | 2004 |
| GOB Product Ltd | Wholesale & retail | 2025 |
| Gofasterwith.Ai Limited | Professional & scientific | 2026 |
| Gofibre Broadband Limited | Information & communication | 2022 |
| Goldenstarberwick Ltd | Hospitality | 2023 |
| Goodfellow Farming Limited | Administrative & support | 2011 |
| Goodyer Scientific Instruments Limited | Wholesale & retail | 1995 |
| Gorse Flower Limited | Hospitality | 2025 |
| Goswick Station Cottages and Old Chapel Residents CIC | Water & waste | 2025 |
| Gourmet Village Inns Limited | Hospitality | 2002 |
| Governors Gardens Management Limited | Households | 2014 |
| Greaves West & Ayre (Berwick) LLP | | 2016 |
| Greaves West & Ayre Limited | Professional & scientific | 2008 |
| Green Berwick Limited | Wholesale & retail | 2022 |
| Green Commercial Limited | Professional & scientific | 2012 |
| Green Engine Limited | Administrative & support | 2015 |
| Greenhill Farm Contractors Ltd | Administrative & support | 2010 |
| Greenhorn Creative Ltd | Education | 2012 |
| Gregson Farming | Administrative & support | 2011 |
| GRF Farms Limited | Professional & scientific | 1996 |
| GRF Land Limited | Wholesale & retail | 1988 |
| GRF Property Limited | Extraterritorial | 2013 |
| Grieve Decor Limited | Construction | 2009 |
| Grindon Rigg Management Limited | Households | 1997 |
| GSY Pearl Ltd | Real estate | 2017 |
| GT Inspections Ltd | Professional & scientific | 2025 |
| Guthrie Care Limited | Health & social care | 2024 |
| GUZ 1 Ltd | Real estate | 2020 |
| GWA Cosec Ltd | Professional & scientific | 2004 |
| Gyntex Limited | Wholesale & retail | 2025 |
| H&S Trade Solutions Ltd | Construction | 2026 |
| Hamilton Organic Limited | Agriculture & fishing | 2008 |
| Hand in Hand Solutions Ltd | Information & communication | 2023 |
| Hanover Credit Limited | Professional & scientific | 1936 |
| Hartech Solutions Limited | Information & communication | 2010 |
| Hassendean Bank Farming Limited | Administrative & support | 2012 |
| Hedley Estates Limited | Real estate | 2016 |
| HEK Music Limited | Information & communication | 2025 |
| Henderson Kildavaig Borders Ltd | Professional & scientific | 2020 |
| HI Barber Ltd | Other services | 2026 |
| HK Consulting (PT) Ltd | Financial services | 2016 |
| Hodgson + White Ltd | Professional & scientific | 2017 |
| Holiday Holmes Limited | Construction | 2016 |
| Holy Island Clothing Co. Limited | Wholesale & retail | 2024 |
| Honeyb Holiday Cottages Ltd | Hospitality | 2025 |
| Horse Direct Limited | Wholesale & retail | 2014 |
| Howden Eggs Limited | Administrative & support | 2016 |
| HTD Nails Ltd | Other services | 2025 |
| HVL Nails Ltd | Other services | 2025 |
| I. & C. (Contracting) Limited | Administrative & support | 2003 |
| IAN O'Rourke & Co Ltd | Professional & scientific | 2014 |
| Iglo Properties Ltd | Construction | 2017 |
| Image NEO Limited | Manufacturing | 2016 |
| Indica Holdings Ltd | Construction | 2017 |
| Indica Properties Ltd | Construction | 2016 |
| Infinity Access Limited | Other services | 2025 |
| IRS Projects Ltd. | Transport & logistics | 2012 |
| IVY Network Technology U.K. Limited | Information & communication | 2006 |
| J a Gibson Ltd. | Wholesale & retail | 2013 |
| J Buckett Ltd | Professional & scientific | 2021 |
| J Richardson Groundworks Ltd | Construction | 2017 |
| J S Crawford Properties (Berwick) Ltd | Construction | 2015 |
| J. & L. Leisure (Amusements) Limited | Other services | 1975 |
| J. Shedden Landscape Ltd | Administrative & support | 2006 |
| J.J. Homes Limited | Agriculture & fishing | 2009 |
| J.K.T Engineering & Fabrication Ltd | Manufacturing | 2019 |
| J.Nicholl Rope Access Limited | Construction | 2018 |
| J.P. Simpson & Co. (Export) Limited | Extraterritorial | 1976 |
| J.P. Simpson and Company (Alnwick) Limited | Extraterritorial | 1954 |
| J.R.Temple & Sons,Limited | Agriculture & fishing | 1939 |
| Jack Brunsdon & SON Limited | Construction | 1964 |
| JAG Art 82 Ltd | Wholesale & retail | 2023 |
| Jaivin Ltd | Information & communication | 2022 |
| James Straughan & Sons Limited | Agriculture & fishing | 1925 |
| Jane Clark Physio Ltd | Health & social care | 2008 |
| JB Clark Electrical Ltd | Construction | 2019 |
| Jcoulsonchef Ltd | Administrative & support | 2025 |
| JG Social Work Services Limited | Health & social care | 2008 |