| Vector Property Estates Limited | Real estate | 2024 |
| Vega Intelligence Ltd | Information & communication | 2026 |
| Venosa Properties Holdings Limited | Construction | 2004 |
| Verandah Berko Ltd | Hospitality | 2017 |
| Verbatim Pharma Ltd | Administrative & support | 2017 |
| Verbatim Services Group Ltd | Financial services | 2023 |
| Verified Free Distribution Limited | Professional & scientific | 1981 |
| Vermillion Analytics Ltd | Administrative & support | 2023 |
| Versilia Consulting Limited | Professional & scientific | 2022 |
| Vertical Capital Ltd | Financial services | 2025 |
| VET Locums Limited | Administrative & support | 2017 |
| VF (Stewkley) Limited | Construction | 2024 |
| Vfinance Limited | Professional & scientific | 2013 |
| VH Collective Limited | Professional & scientific | 2023 |
| Vibrant Lifestyle Limited | Wholesale & retail | 2019 |
| Vicky Bodhani Ltd | Professional & scientific | 2025 |
| Victoria Street Chapel Residents Company Limited | Real estate | 2004 |
| Village B Limited | Real estate | 2020 |
| Village Foundations Limited | Real estate | 2016 |
| Virtual AI Limited | Professional & scientific | 2017 |
| Vis-A-Vis Video Limited | Information & communication | 2004 |
| Vision (Slpf) Investments Ltd | Real estate | 2021 |
| Vision Contracts Ltd | Construction | 2007 |
| Visot Limited | Transport & logistics | 2021 |
| Vita Media Limited | Information & communication | 2001 |
| Vitale & Stocks Limited | Real estate | 2018 |
| Vitalit Ltd | Information & communication | 2026 |
| Vitality Consultancy Services Ltd | Professional & scientific | 2005 |
| Vivid Sourcing Ltd | Professional & scientific | 2018 |
| Voice Over Services Ltd | Information & communication | 2021 |
| Volna Capital Limited | Professional & scientific | 2026 |
| Vossers Ltd | Information & communication | 2008 |
| Voucher Networks Ltd | | 2011 |
| VRO Marketing Ltd | Professional & scientific | 2024 |
| Vukovision Limited | Information & communication | 2025 |
| Waggle Ltd | Other services | 2010 |
| Walking With Eris CIC | Health & social care | 2024 |
| Wamimichi Berkhamsted Ltd | Hospitality | 2024 |
| Warby Property Search Ltd | Real estate | 2021 |
| Warehouse Pizza Limited | Hospitality | 2021 |
| Warmfield (Ferrybridge) Two Limited | Real estate | 2020 |
| Warren Hoadly Painters & Decorators Ltd | Construction | 2020 |
| Waterhouse Property Holdings Limited | Financial services | 2019 |
| Waterloo 200 Limited | Education | 2007 |
| Waterway Recovery Group Limited | Administrative & support | 2003 |
| Watson Willis Consulting Limited | Professional & scientific | 2023 |
| WE ARE the Lift Ltd | Professional & scientific | 2024 |
| WE ARE Unchained Ltd | Professional & scientific | 2020 |
| WEE Beastie Gardens Ltd | Professional & scientific | 2026 |
| Welcome Home Group Ltd | Financial services | 2026 |
| Wendover ARM Trust | Other services | 1989 |
| Wendover Canal Restoration Limited | Administrative & support | 2022 |
| Wesavor Ltd | Hospitality | 2022 |
| West Herts 21 Century Hospital Solution | Other services | 2017 |
| WGD Brickwork Ltd | Construction | 2022 |
| What 2B. Ltd | Wholesale & retail | 2021 |
| Wheelfix DOC Ltd | Wholesale & retail | 2026 |
| Wheyd Ltd | Manufacturing | 2017 |
| White Hill Residents Company (Berkhamsted) Limited | Administrative & support | 1973 |
| White Island Limited | Other services | 2021 |
| White Label Network Ltd | Administrative & support | 2012 |
| White Wallpaper Architecture Limited | Professional & scientific | 2017 |
| Who's the Daddy? Ltd | Administrative & support | 2012 |
| Whole Works Ltd | Professional & scientific | 2021 |
| Wilbur & Gussie Limited | Wholesale & retail | 2005 |
| Wilcock Associates Ltd | Education | 2018 |
| Wild Herz Travel Ltd | Administrative & support | 2025 |
| Wild Service Limited | Agriculture & fishing | 1998 |
| Willerby Commercial Properties Limited | Real estate | 2025 |
| Williams Fit Ltd | Health & social care | 2018 |
| Willow Medical Ltd | Health & social care | 2020 |
| Willow Sway Sound Limited | Information & communication | 2025 |
| Winchmore Hill Investments Ltd | Financial services | 2012 |
| Windhawk Consulting Ltd | Professional & scientific | 2014 |
| Winning by Thinking Limited | Professional & scientific | 2005 |
| Wired Communications Limited | Professional & scientific | 2018 |
| Wise-Aid | Health & social care | 1997 |
| With US for YOU Ltd | Real estate | 2014 |
| Wmlh Limited | Administrative & support | 2018 |
| Woburn Consultancy Limited | Professional & scientific | 2024 |
| Wolfhunter Films Ltd | Administrative & support | 2012 |
| Womb to Bloom Limited | Health & social care | 2010 |
| Woodburn Property Group Limited | Real estate | 2025 |
| Woodface Limited | Professional & scientific | 2007 |
| Woodfield Consulting Limited | Construction | 2023 |
| Woodlands AVE Ltd | Construction | 2019 |
| Woodstock English Honey Limited | Manufacturing | 2018 |
| Woom Bikes UK Ltd | Wholesale & retail | 2025 |
| Words With Ideas Ltd | Professional & scientific | 2017 |
| Workplace Unlimited Ltd | Professional & scientific | 2012 |
| Workplace Wellbeing Academy Ltd | Professional & scientific | 2020 |
| Worktop Warehouse Limited | Wholesale & retail | 2025 |
| Worktop Warehouse Wholesale Limited | Other services | 2025 |
| World to Come Limited | Wholesale & retail | 2019 |
| Worthy of Remark Ltd | Other services | 2026 |
| WSA Construction Limited | Construction | 2019 |
| Wyndham Ltd | Households | 2019 |
| X Dells Removals Ltd | Transport & logistics | 2023 |
| XKE Capital LLP | | 2010 |
| Xtra Opportunities Limited | Professional & scientific | 2017 |