| DR SUE Smith Ltd | Professional & scientific | 2026 |
| Dracar Ltd | Construction | 2019 |
| Dragintra Fleet Services UK Ltd | Administrative & support | 2016 |
| Drainstore Limited | Construction | 2004 |
| Drainstore Pumping Limited | Manufacturing | 2016 |
| Dray Ltd | Real estate | 2016 |
| Draycott Mills Ltd | Real estate | 2025 |
| Dreamfusion Productions Limited | Information & communication | 2011 |
| Drew Smith Medical Services Limited | Health & social care | 2023 |
| Drew Smith Property Services Limited | Real estate | 2024 |
| Drisilane Ltd | Wholesale & retail | 2021 |
| Dsmtcc Limited | Health & social care | 2025 |
| Duffield Balti & Grillz Ltd | Hospitality | 2025 |
| Duffield Chiropractic Clinic Limited | Health & social care | 2017 |
| Duffield Dental Practice Limited | Health & social care | 2006 |
| Duffield Garage Ltd | Wholesale & retail | 2024 |
| Duffield Grill & Balti Ltd | Hospitality | 2022 |
| Duffield News Limited | Wholesale & retail | 2015 |
| Duffield Park Management Company (1997) Limited | Real estate | 1997 |
| Duffield Property Limited | Real estate | 2025 |
| Dunking Churros Limited | Hospitality | 2023 |
| DWS Excavations and Groundworks Ltd | Construction | 2022 |
| E Bradshaw Publishing Ltd | Information & communication | 2017 |
| E&Pda Limited | Hospitality | 2025 |
| E.R.M Property Management Ltd | Households | 2025 |
| Early Glimpse Ltd | Professional & scientific | 2022 |
| East Midlands Promo Print Limited | Manufacturing | 2018 |
| East Midlands Swimming Academy Limited | Education | 2016 |
| Easy Accounts Limited | Professional & scientific | 2005 |
| Easy-Gate Limited | Manufacturing | 1999 |
| Eatonbank Limited | Professional & scientific | 2006 |
| ECB Services Amber Valley Ltd | Professional & scientific | 2018 |
| Ecclesbourne Reach Management Company Limited | Real estate | 2010 |
| Eco Tree Company UK Limited | Agriculture & fishing | 2008 |
| Eden House Holdings Ltd | Health & social care | 2019 |
| Edg-Tek Ltd | Professional & scientific | 2022 |
| Edgar Jarvis Ltd | Wholesale & retail | 2020 |
| EFK Business Solutions Ltd | Administrative & support | 2022 |
| EL Capo's Pizza Limited | Hospitality | 2022 |
| Elearning Laboratory Ltd | Information & communication | 2012 |
| Electr-Otel Ltd | Construction | 2025 |
| Electric Gate Servicing Ltd | Administrative & support | 2022 |
| Electrical Design Solutions Ltd | Professional & scientific | 2023 |
| Electrisol Limited | Energy | 2013 |
| Elegant Baby Limited | Extraterritorial | 2020 |
| Elevation Group Ltd | Professional & scientific | 2013 |
| Elite Home Improvements (Derbyshire) Ltd | Construction | 2022 |
| Ellewis Ltd | Extraterritorial | 2021 |
| Elmsby Limited | Manufacturing | 2025 |
| EM2 Developments Ltd | Real estate | 2014 |
| Emcus Limited | Professional & scientific | 2009 |
| Emma Wood Limited | Other services | 2011 |
| Enable Services Group Limited | Administrative & support | 2021 |
| Encole Management Systems Ltd | Professional & scientific | 2020 |
| ENS Site Services Limited | Manufacturing | 2025 |
| Enterprise Advisors (UK) Limited | Professional & scientific | 2008 |
| Envirodrain Limited | Extraterritorial | 2010 |
| EOS Leadership Ltd | Professional & scientific | 2019 |
| Epona Invests Limited | Real estate | 2017 |
| EPP (E&W) Ltd | Professional & scientific | 2020 |
| Esland North Limited | Health & social care | 2001 |
| Esland South Limited | Health & social care | 2003 |
| Euphoria Estates Limited | Real estate | 2022 |
| Ev-Tech Systems Limited | Information & communication | 2009 |
| Evans and Peel Limited | Hospitality | 2011 |
| Eventus Care Group Limited | Health & social care | 2025 |
| Evermore Video Portraits Ltd | Information & communication | 2025 |
| Evershine (Belper) Limited | Other services | 2008 |
| Everything Organised Events Ltd | Other services | 2015 |
| EWB Consulting Limited | Information & communication | 2009 |
| EX and HAM Ltd | Real estate | 2021 |
| Exciting Times Ltd | Education | 2023 |
| Exell Flooring Limited | Construction | 2006 |
| Expert Independent Surveys Ltd | Professional & scientific | 2022 |
| Eyden Signs Limited | Construction | 2006 |
| F M Ottewell & SON Limited | Agriculture & fishing | 2008 |
| F Sullivan Ltd | Extraterritorial | 2011 |
| F.A.R.T Goblin Tech Ltd | Wholesale & retail | 2025 |
| Fabrini Ltd | Professional & scientific | 2005 |
| Face UP Theatre Ltd | Arts & recreation | 2015 |
| Factory Campers Limited | Extraterritorial | 2024 |
| Fagin's Loft Ltd | Wholesale & retail | 2026 |
| Fairfield Aviation Engineering Ltd | Manufacturing | 2013 |
| Fairview Grange 1970 Limited | Extraterritorial | 2015 |
| Falconbridge Services Ltd | Professional & scientific | 2024 |
| Fancollectables Ltd | Wholesale & retail | 2021 |
| Fantastic MR. Box Limited | Professional & scientific | 2023 |
| Farmfluence Limited | Information & communication | 2025 |
| Farnah Investments Limited | Real estate | 2008 |
| Farsight Recruitment Holdings Limited | Financial services | 2018 |
| FAT Daddy's Catering Ltd | Hospitality | 2026 |
| FB 88 Limited | Professional & scientific | 2010 |
| FD Power 1 Ltd | Professional & scientific | 2024 |
| FD Power 2 Ltd | Professional & scientific | 2024 |
| FD Power 3 Ltd | Professional & scientific | 2025 |
| Fern & Fable Ltd | Manufacturing | 2024 |
| Fernbank Holdings Ltd | Real estate | 2020 |
| Ferrybridge DC Holdco Limited | Financial services | 2024 |
| Ferrybridge DC Topco Limited | Financial services | 2024 |
| FFF Matt Bailey Limited | Arts & recreation | 2025 |