| Doncog Joiners Ltd | Construction | 2023 |
| Doon Valley Property Ltd. | Real estate | 1999 |
| Doris Signalling Limited | Other services | 2017 |
| DPS IT Solutions Ltd | Information & communication | 2023 |
| DR Golf Professional Ltd. | Wholesale & retail | 2011 |
| Drakemuir Ltd | Wholesale & retail | 2023 |
| Drambuie Limited | Professional & scientific | 1990 |
| Drambuie Liqueur Company Ltd. (the) | Manufacturing | 1927 |
| Dslcouriersdsp Ltd | Transport & logistics | 2025 |
| Dsvm Property Ltd | Real estate | 2025 |
| DTA Technical Limited | Other services | 2014 |
| DTP Projects (Plumbing & Heating) Ltd | Construction | 2018 |
| DUA Properties Scot Ltd | Real estate | 2024 |
| Duffy Mitchell & Wells Ltd | Extraterritorial | 2015 |
| Duncan Advisory Ltd | Professional & scientific | 2015 |
| Dundas Decorating Ltd | Construction | 2024 |
| Dundee Projects Ltd | Professional & scientific | 2023 |
| Dunfermline Care Home Ltd | Real estate | 2022 |
| Dupuy Frame Ltd | Construction | 2018 |
| DX Academy Ltd | Education | 2023 |
| DX Property Group Ltd | Real estate | 2023 |
| Dxhi Group Ltd | Construction | 2021 |
| E4I Holdings Limited | Construction | 2008 |
| E4I Schools Limited | Construction | 2008 |
| Easi Uplifts (Aerials) Limited | Administrative & support | 1998 |
| Easi Uplifts (Specialist Access) Limited | Administrative & support | 2010 |
| Eastern Foods Bellshill Ltd | Wholesale & retail | 2022 |
| Ebbe Property Ltd | Construction | 2021 |
| EBS Surveying Ltd | Professional & scientific | 2024 |
| ECI Solutions (Scotland) Ltd | Manufacturing | 2019 |
| Eco Solutions Group Scotland Ltd | Professional & scientific | 2022 |
| Ecoheat Glasgow Ltd | Construction | 2024 |
| Ecosse Chevrons Limited | Manufacturing | 2026 |
| Ecosse Enterprise Ltd | Transport & logistics | 2024 |
| Ecosse Graphics Limited | Manufacturing | 2026 |
| Ecosse Signs Ltd | Manufacturing | 2009 |
| Ecosse Wraps Limited | Manufacturing | 2026 |
| Ecowaste Ltd | Wholesale & retail | 2018 |
| Eisa Technical and Enviromental Ltd. | Construction | 2025 |
| EK 482GB Ltd | Wholesale & retail | 2023 |
| Electricaire Limited | Construction | 2006 |
| Elite Fitness Club Ltd | Arts & recreation | 2017 |
| EMP Systems Limited | Professional & scientific | 2015 |
| Energyhouse Scot Ltd | Energy | 2010 |
| Enterobiotix Limited | Professional & scientific | 2017 |
| Envevo Civil Engineering Limited | Professional & scientific | 2022 |
| Envious Installations Ltd | Construction | 2021 |
| Envirocure (Scotland) Limited | Administrative & support | 2024 |
| Enviroteam Services Ltd | Water & waste | 2017 |
| Equitix Chariot (S) GP Limited | Financial services | 2019 |
| Equitix Chariot (S) LP | | 2019 |
| Equitix Exeter (S) III GP Limited | Real estate | 2016 |
| Equitix Exeter (S) III LP | | 2016 |
| Equitix Fire Control (Durham) (S) IV GP Limited | Real estate | 2015 |
| Equitix Fire Control (Durham) (S) IV LP | | 2015 |
| Equitix Fire Control (S) IV GP Limited | Real estate | 2015 |
| Equitix Fire Control (S) IV LP | | 2015 |
| Equitix Grimsby (S) MA 1 GP Limited | Real estate | 2016 |
| Equitix Grimsby (S) MA 1 LP | | 2016 |
| Equitix NCP (S) LP | | 2017 |
| Equitix NCP Scottish GP Limited | Professional & scientific | 2017 |
| Equitix NVS (S) LP | | 2020 |
| Equitix NVS Scottish GP Limited | Professional & scientific | 2020 |
| Equitix Renaissance (S) IV GP Limited | Real estate | 2015 |
| Equitix Renaissance (S) IV LP | | 2015 |
| Equitix Social Housing (S) IV LP | | 2016 |
| Equitix Tiger (S) LP | | 2018 |
| Equitix Tiger Scottish GP Limited | Financial services | 2018 |
| ESG Tech Serv Limited | Manufacturing | 2012 |
| Espardo Tech Limited | Wholesale & retail | 2022 |
| Euro Steel Shutters Ltd | Manufacturing | 2016 |
| European Trailer Services Limited | Transport & logistics | 2020 |
| EV Capital Ltd | Financial services | 2026 |
| Eva's Aesthetic Limited | Other services | 2024 |
| Eve's Kitchen Ltd | Hospitality | 2026 |
| Eveross Holdings Ltd | Water & waste | 2022 |
| EVO Tech SCO Ltd | Professional & scientific | 2025 |
| Excelarate Limited | Professional & scientific | 2020 |
| Exilux Solutions Ltd | Wholesale & retail | 2024 |
| Expert Heating Scotland Ltd | Construction | 2015 |
| F-Audio Limited | Manufacturing | 2017 |
| Fabuluxe Hair Ltd | Other services | 2026 |
| Faiqa Tahir Jathool Ltd | Wholesale & retail | 2024 |
| Fair Price (Glasgow) Ltd | Wholesale & retail | 2026 |
| Fairdeal Skill Ltd | Wholesale & retail | 2013 |
| Fantasy Forge Ltd | Manufacturing | 2025 |
| Fast Fit Services Ltd | Wholesale & retail | 2023 |
| Fdbd Scotland Ltd Ltd | Wholesale & retail | 2024 |
| Ferniegair Property Matters Ltd | Real estate | 2022 |
| FES Resources Limited | Construction | 2006 |
| Fever Pitch Academy Ltd | Arts & recreation | 2022 |
| FIG Toy Funhouse Ltd | Wholesale & retail | 2024 |
| Fine Choice Catering Ltd | Hospitality | 2026 |
| Finishing Touches (Bellshill) Limited | Other services | 2006 |
| Firefly Enterprises Ltd. | Information & communication | 2002 |
| Five Star Football Events Limited | Transport & logistics | 2017 |
| Five Star International Events Limited | Arts & recreation | 2020 |
| Fletcher Lettings Limited | Real estate | 2013 |
| Football FUN Factory (Community 110) Limited | Arts & recreation | 2023 |
| FOR Fudge Shakes Ltd | Hospitality | 2022 |