| 3D Garden Design Limited | Administrative & support | 2005 |
| Acfx Trades Ltd | Professional & scientific | 2024 |
| Acmca Limited | Financial services | 2002 |
| Aggregate Remade Ltd. | Manufacturing | 2013 |
| Ailsa Engineering Ltd | Professional & scientific | 2024 |
| Ajgtlogistics Ltd | Other services | 2025 |
| Alliance Wine Company Limited | Wholesale & retail | 1984 |
| Alliance Wine Group Limited | Financial services | 2017 |
| Andrew Irvine Ltd | Real estate | 2024 |
| Anglo Scottish Cement Limited | Manufacturing | 2004 |
| Art & Earth Collective CIC | Professional & scientific | 2024 |
| Ashton Marine Services Ltd | Wholesale & retail | 2016 |
| ATB Drilling Limited | Construction | 2014 |
| Ayrshire Services Ltd | Construction | 2022 |
| Barking MAD Bistro Ltd | Wholesale & retail | 2020 |
| Barrmill Plant Services Limited | Construction | 2007 |
| BAT Surveys Ltd | Professional & scientific | 2025 |
| BEEPLACE2BEE Limited | Hospitality | 2025 |
| Beith Community Development Trust | Arts & recreation | 2011 |
| Beith General Store Ltd | Wholesale & retail | 2026 |
| Beith Golf Club (2023) Ltd | Arts & recreation | 2023 |
| Beith Hive Childcare Ltd | Health & social care | 2023 |
| Beith Logistics Ltd | Transport & logistics | 2015 |
| Biglees Quarry Ltd | Real estate | 2022 |
| Birch Windows & Doors Limited | Construction | 2022 |
| Blue Sky Software Testing Limited | Professional & scientific | 2007 |
| BS MOT Station Limited | Wholesale & retail | 2018 |
| Burnhouse Caravans Limited | Wholesale & retail | 2013 |
| Burnhouse Engineering & Fabrication Limited | Manufacturing | 1998 |
| Burnhouse Properties Limited | Real estate | 2023 |
| Campbell Mcginn Ltd | Real estate | 2026 |
| Camsail Estates Ltd | Financial services | 2021 |
| Chemtec UK Limited | Manufacturing | 2004 |
| CJB Goods and Services Limited | Wholesale & retail | 2024 |
| Cjbrown Hospitality Ltd | Hospitality | 2025 |
| Clark Family Investments Limited | Real estate | 1992 |
| Concrete Scotland Limited | Financial services | 2024 |
| Covepoint Limited | Professional & scientific | 2014 |
| Crawford Property Services Scotland Limited | Real estate | 2025 |
| Crawford Transport Services Limited | Wholesale & retail | 2013 |
| Crownloch Limited | Water & waste | 2013 |
| Croy HR Consultancy Limited | Professional & scientific | 2025 |
| D.W.Morrison Ltd | Construction | 2021 |
| Dcs.Home Improvements Ltd | Construction | 2025 |
| Deck Holdings Ltd | Real estate | 2023 |
| Derelict Organic Ltd | Wholesale & retail | 2024 |
| Double Crown Healthcare Ltd | Administrative & support | 2022 |
| Drfm Limited | Professional & scientific | 2021 |
| Dunmuir Coachworks Ltd | Wholesale & retail | 2019 |
| ECL Property Management Ltd | Households | 2018 |
| Eclipse Purchasing Limited | Wholesale & retail | 1988 |
| Eco Skips (Scotland) Limited | Water & waste | 2012 |
| Ecoscreed Group Limited | Manufacturing | 2012 |
| Eddie Gray Driver Training Limited | Education | 2025 |
| Ellwoods Pizza Limited | Hospitality | 2026 |
| Embroidery Direct Limited | Transport & logistics | 2004 |
| EP Motors Ltd | Wholesale & retail | 2026 |
| Event Comms Ltd | Information & communication | 2021 |
| F & B Building Scotland Limited | Construction | 2014 |
| F.A. Parkinson (Chemists) Limited | Professional & scientific | 1963 |
| Future Form (SPF) Limited | Manufacturing | 2025 |
| Garnock Distribution Limited | Wholesale & retail | 2023 |
| Garnock Valley Music Network CIC | Information & communication | 2024 |
| Gary Whitehill Limited | Hospitality | 2021 |
| Gateside Nursery Limited | Wholesale & retail | 2018 |
| Geilsland Enterprise Ltd | Hospitality | 2024 |
| Glencairn Commercial Ltd | Water & waste | 2021 |
| Go West Sailing Ltd | Arts & recreation | 2017 |
| Hannah Jackson Beauty Ltd | Other services | 2020 |
| Harkin Hospitality Ltd | Hospitality | 2021 |
| Hidden GEM Equestrian Ltd | Wholesale & retail | 2023 |
| Homestay Property Management Ltd | Households | 2024 |
| Ibeitha Limited | Hospitality | 2024 |
| Immo Services Ltd | Real estate | 2023 |
| Infinity Cloud Ltd | Administrative & support | 2012 |
| Intelligent Security Solutions Ltd | Manufacturing | 2021 |
| Invest & Refurb Ltd | Financial services | 2025 |
| Irvine's Limited | Manufacturing | 2006 |
| Islesat Ltd | Construction | 2015 |
| Jetcut Ltd. | Manufacturing | 2005 |
| JLK Properties Limited | Real estate | 1991 |
| JNS Hotels Ltd | Hospitality | 2016 |
| Joan Services Limited | Transport & logistics | 2012 |
| John Shepherd Transport Ltd. | Transport & logistics | 2003 |
| Jukebox Creative Ltd | Professional & scientific | 2018 |
| Julia Fields Interiors Ltd. | Wholesale & retail | 1978 |
| K Blackwood Plumbing and Heating Ltd | Construction | 2024 |
| K&O Deliveries Ltd | Transport & logistics | 2022 |
| KAI Reesu Ltd | Wholesale & retail | 2025 |
| Kerr Cleaning Services Ltd | Administrative & support | 2024 |
| Kleen Cutz Ltd | Other services | 2025 |
| Ko’Dd Contracts Ltd | Construction | 2022 |
| KRC Contracting Ltd | Manufacturing | 2016 |
| Lanoshield Limited | Professional & scientific | 2021 |
| Laraworks Ltd | Wholesale & retail | 2021 |
| Lennon Lodges Ltd | Real estate | 2023 |
| LEX Scotland CIC | Professional & scientific | 2023 |
| LGC Legal Limited | Professional & scientific | 2025 |
| Lochfaulds Logistics Ltd | Transport & logistics | 2024 |
| Lordan Homes Ltd | Real estate | 2025 |