| Oakwood Architecture Ltd | Professional & scientific | 2023 |
| Oantadab Ltd | Professional & scientific | 2020 |
| Oarpel Community Support Limited | Administrative & support | 2023 |
| Oasis Detailing Ltd | Wholesale & retail | 2022 |
| Oasis of Care and Support Ltd | Administrative & support | 2024 |
| Oasis Smart Repairs Ltd | Wholesale & retail | 2026 |
| Oasis Valeting Bedford Ltd | Wholesale & retail | 2018 |
| Oatland House Care Ltd | Health & social care | 2022 |
| OB City Football Club Ltd | Arts & recreation | 2025 |
| OB Developer Services Ltd | Professional & scientific | 2026 |
| Ob's Store Ltd | Wholesale & retail | 2021 |
| Obaloluwa Enterprises Limited | Health & social care | 2018 |
| OBC Lavendon Ltd | Extraterritorial | 2018 |
| Oberon Court (Bedford)Residents' Association Limited | Households | 1969 |
| Objectmark Limited | Wholesale & retail | 1995 |
| Oblivine Ltd | Wholesale & retail | 2025 |
| Obscure Rebellion Ltd | Wholesale & retail | 2014 |
| Obsidian & Oak Ltd | Real estate | 2025 |
| Obsidian Spaces Ltd | Real estate | 2022 |
| Obsidianet Ltd | Information & communication | 2022 |
| Ocarina OT Limited | Financial services | 2021 |
| Occas Limited | Professional & scientific | 2004 |
| Ocean Blue Entertainment Limited | Hospitality | 2024 |
| Ocean Creative Corporation Ltd | Professional & scientific | 2020 |
| Ocean Creative Ltd. | Professional & scientific | 2006 |
| Ocean Frog Ltd | Wholesale & retail | 2023 |
| Ocean Shore One Limited | Hospitality | 2021 |
| Oceanablue Consulting Limited | Information & communication | 2009 |
| Oceanchem Export Limited | Wholesale & retail | 2014 |
| Octagon Recruitment Group Ltd | Administrative & support | 2018 |
| Octagon Technical Services Limited | Professional & scientific | 2007 |
| Octavian Consultancy Ltd | Professional & scientific | 2014 |
| Octec Consulting Ltd | Administrative & support | 2016 |
| Octosciences Limited | Manufacturing | 2026 |
| Octua Consulting Limited | Professional & scientific | 2026 |
| Od&C Associates Ltd | Professional & scientific | 2025 |
| ODC Beauty Clinic Aesthetic Medicine Ltd | Other services | 2024 |
| ODC Beauty Clinic Ltd | Other services | 2018 |
| ODD Jobs Handyman Services Limited | Administrative & support | 2017 |
| Odell Close Residents Company Limited | Households | 1982 |
| Odonata Business Services Limited | Information & communication | 2017 |
| Odra Bedford Ltd | Wholesale & retail | 2018 |
| ODT Used Parts Ltd | Wholesale & retail | 2025 |
| Odyssey Imports Limited | Wholesale & retail | 2017 |
| Oferseon Limited | Professional & scientific | 2012 |
| OFF Beat Pictures Limited | Information & communication | 2022 |
| OFF Brief Strategy Ltd | Professional & scientific | 2025 |
| OFF Grid Ltd | Financial services | 2011 |
| OFF Grid Powergen Limited | Administrative & support | 2014 |
| OFF the Record TV Ltd | Manufacturing | 2026 |
| OFF the Shelf Technology Ltd | Information & communication | 2017 |
| Office Unlocked Limited | Information & communication | 2006 |
| Office Wings Limited | Administrative & support | 2012 |
| Officedine-In Limited | Hospitality | 2023 |
| Official Plates Ltd | Wholesale & retail | 2020 |
| Offshore Aerial Survey Services Ltd | Manufacturing | 2018 |
| Offshore Fibre Solutions Limited | Manufacturing | 2013 |
| Ofme Management Ltd | Professional & scientific | 2024 |
| Oggy Labs Ltd | Information & communication | 2025 |
| OH My GAS Ltd | Construction | 2023 |
| OH Very Social Limited | Professional & scientific | 2024 |
| OH03GROUP Ltd | Transport & logistics | 2021 |
| Ohana FDN Ltd | Professional & scientific | 2024 |
| Ohanapropertysolutions Ltd | Construction | 2025 |
| OHG Plumbing and Heating Services Ltd | Construction | 2023 |
| OHJ Services Ltd | Financial services | 2026 |
| OIL247 Limited | Wholesale & retail | 2017 |
| OJH Geological Limited | Mining & quarrying | 2017 |
| Ojpolo Limited | Extraterritorial | 2010 |
| OJR Care Limited | Health & social care | 2025 |
| Okapi Enterprise Limited | Hospitality | 2012 |
| Okelue Care Limited | Health & social care | 2015 |
| Okolo Limited | Professional & scientific | 2016 |
| Okomek Transport Ltd | Transport & logistics | 2018 |
| Oktober Hair Ltd | Other services | 2023 |
| Okupski Trade Ltd | Transport & logistics | 2016 |
| Olap Autos Limited | Wholesale & retail | 2024 |
| Old Black Horse Residents Limited | Households | 2015 |
| Old Lane (Ockham) Management Company Limited | Households | 2008 |
| Old Oak Legal Ltd | Information & communication | 2023 |
| Old Reliable Ltd | Information & communication | 2020 |
| Old Road Securities Holdings Ltd | Financial services | 2023 |
| Old Road Securities Limited | Agriculture & fishing | 1973 |
| Old Vicarage Advisory Legal (Kempston) Limited | Professional & scientific | 2011 |
| Oldhill Construction Limited | Construction | 2025 |
| OLE Transport Limited | Transport & logistics | 2021 |
| Olena Baker Health Limited | Education | 2025 |
| Olive Bedford Ltd | Hospitality | 2021 |
| Olive Mortgages Ltd | Financial services | 2026 |
| Oliver Clifton Management Limited | Households | 2008 |
| Oliver Rene Ltd | Real estate | 2020 |
| Oliver Russell Property Limited | Real estate | 2009 |
| Olivier and Union Ltd | Real estate | 2022 |
| Olney Bathrooms Limited | Construction | 2013 |
| Olneyinks Limited | Wholesale & retail | 2025 |
| Olushola Physio Limited | Health & social care | 2022 |
| Oluwaseun Caleb Adebolu Ltd | Health & social care | 2020 |
| Olympia Architecture Ltd | Professional & scientific | 2018 |
| OM Saravana Bahava Ltd | Real estate | 2024 |
| Ombre Cosmetics Limited | Wholesale & retail | 2025 |