| Crow Consulting Ltd | Information & communication | 2023 |
| CSW Online Limited | Information & communication | 2014 |
| CSW Online Shop Ltd | Wholesale & retail | 2018 |
| D a Manufacturing Ltd | Manufacturing | 2017 |
| D'S Mobile Ltd | Hospitality | 2022 |
| D. B. Earthmoving & Plant Hire Limited | Agriculture & fishing | 1973 |
| D1 Consulting Limited | Professional & scientific | 2016 |
| DAN Gale Golf Limited | Wholesale & retail | 2015 |
| Danny Hill Developments Limited | Construction | 2018 |
| Danny Hill Holdings Ltd | Financial services | 2021 |
| Danson Tree Care Limited | Agriculture & fishing | 2013 |
| Dark Media Publishing Limited | Information & communication | 2023 |
| David Wicks Golf School Ltd | Education | 2023 |
| DB Electrical (Sussex) Limited | Construction | 2026 |
| DC ERP Consulting Ltd | Information & communication | 2025 |
| DC Refurbishments Ltd | Real estate | 2025 |
| Deepsheep Limited | Arts & recreation | 2024 |
| Delta's Snack Bar Ltd | Hospitality | 2025 |
| Dennis Thomas Builders Limited | Construction | 2006 |
| Design-A-Canvas Ltd | Manufacturing | 2009 |
| Designs Incorporated Limited | Professional & scientific | 2000 |
| Digital Production Partnership Limited | Information & communication | 2015 |
| Dimora Consultancy Ltd | Professional & scientific | 2025 |
| Dinarie Limited | Health & social care | 2017 |
| Director Dynamics Limited | Professional & scientific | 2021 |
| Dissonance Quartets Ltd | Education | 2026 |
| Divine Healing Mission (Holdings) Limited | Other services | 1962 |
| DLB Construction SE Limited | Construction | 2016 |
| Domain Support Ltd | Information & communication | 2005 |
| Domaines Development & Construction Ltd | Construction | 2025 |
| DPF Films Limited | Information & communication | 2012 |
| DPP International Limited | Information & communication | 2022 |
| DTS Property Services Limited | Construction | 2020 |
| Durant Property Limited | Administrative & support | 2003 |
| Dyme Accounting Limited | Professional & scientific | 2025 |
| Dyme Holdings Limited | Real estate | 2023 |
| Dyme PI Limited | Real estate | 2025 |
| Dyme Properties Limited | Real estate | 2022 |
| EAS Accounting Limited | Professional & scientific | 2019 |
| East Sussex Holiday Letd Limited | Real estate | 2018 |
| East Sussex Lettings and Management Ltd | Real estate | 2013 |
| East Sussex Logs Ltd | Agriculture & fishing | 2023 |
| East Sussex Refurbishment Limited | Real estate | 2004 |
| Eastbourne Nutrition Ltd | Health & social care | 2025 |
| Eclipse Displays Limited | Construction | 1999 |
| Ecoflo Garden Irrigation Ltd | Administrative & support | 2004 |
| Edward's Property and Craft Ltd | Construction | 2017 |
| Ehez Consulting Limited | Professional & scientific | 2023 |
| Eightangles Ltd | Professional & scientific | 2021 |
| Eightangles UK LLP | | 2019 |
| Elevations Design Limited | Professional & scientific | 2002 |
| Elite Electrical Services and Solutions Ltd | Construction | 2011 |
| Elite Food Machinery Services Ltd | Manufacturing | 2015 |
| Elite Grab Hire Limited | Construction | 2021 |
| Elite Groundworks Ltd | Construction | 2018 |
| Elite Property and Developments Ltd | Real estate | 2022 |
| Ellaich Medical Ltd | Professional & scientific | 2016 |
| Elliott’S Electric Vibe Ltd | Information & communication | 2025 |
| EME Lettings Ltd | Real estate | 2019 |
| Emin Read Solicitors Ltd | Professional & scientific | 2015 |
| Engage for Impact Ltd | Professional & scientific | 2025 |
| English Wine Tasting Tours Limited | Administrative & support | 2021 |
| Enlightened Property Limited | Real estate | 2007 |
| Enneagram Global Ltd | Professional & scientific | 2026 |
| Episystem Limited | Administrative & support | 2020 |
| Equity Transfer Consulting Limited | Professional & scientific | 2014 |
| ESS TEE Squared Limited | Professional & scientific | 2020 |
| Evergreen CS Ltd | Information & communication | 2021 |
| Evie TAN Limited | Other services | 2021 |
| Evolution Property Solutions Limited | Construction | 2022 |
| EWG Engineering Ltd | Professional & scientific | 2015 |
| EWG Property Ltd | Real estate | 2024 |
| Excel Inventories Limited | Administrative & support | 2019 |
| Exotic West Limited | Administrative & support | 2019 |
| F Strickland & Sons Ltd | Wholesale & retail | 2003 |
| Fairmount Residents Association Limited | Households | 2005 |
| Famous Amos Limited | Information & communication | 2013 |
| FAT Cheepy Limited | Real estate | 2003 |
| FE Consulting (Sussex) Ltd | Education | 2016 |
| Fernhill PUB Co. Ltd | Hospitality | 2012 |
| Fifth Element Ltd | Extraterritorial | 2024 |
| Finair Limited | Information & communication | 2010 |
| Fine Turf Management LLP | | 2012 |
| Firststrike Computing Ltd | Administrative & support | 1998 |
| Five Fostering Holdings Ltd | Financial services | 2024 |
| Five Fostering Ltd. | Health & social care | 2012 |
| Flora Villas Properties Ltd | Real estate | 2026 |
| FME Services UK Limited | Manufacturing | 2017 |
| Food Rooms Limited | Hospitality | 2000 |
| Forbes Marine & Logistics Ltd | Transport & logistics | 2014 |
| Fortuna Marketing Ltd | Administrative & support | 2014 |
| Foster Motors Limited | Wholesale & retail | 2000 |
| Freemen of the Blean CIC | Education | 2024 |
| Friends of Barefeet Ltd | Health & social care | 2019 |
| Frobisk Limited | Administrative & support | 2021 |
| Full Spot Ltd. | Administrative & support | 2018 |
| Funk Fusion Fitness Limited | Arts & recreation | 2011 |
| G D Contracting Services Limited | Construction | 1997 |
| G J Standen Limited | Administrative & support | 2025 |
| G.A.Fabrications Ltd | Professional & scientific | 2024 |