| DHC PVT Ltd | Real estate | 2025 |
| DIAMONDS5 Ltd | Hospitality | 2026 |
| Dignan Group Ltd | Information & communication | 2026 |
| Dimension Joinery Ltd | Construction | 2025 |
| Dino Transport Ltd | Transport & logistics | 2022 |
| DIR Designs Ltd | Arts & recreation | 2024 |
| Direct Care Service Limited | Health & social care | 2019 |
| Distell.Com Ltd | Manufacturing | 2013 |
| Distinct Installations Limited | Construction | 2017 |
| DJK Motors Ltd | Wholesale & retail | 2015 |
| DMB Plumbing Ltd | Construction | 2020 |
| Dmssll SW Ltd | Information & communication | 2024 |
| DNS Electrical & Renewables Ltd | Construction | 2025 |
| Dodds Painting & Decorating Ltd | Construction | 2021 |
| Domestic Letting & Management Ltd | Real estate | 2025 |
| DON Consulting UK Limited | Professional & scientific | 2016 |
| Donny's Carpets Limited | Wholesale & retail | 2008 |
| Dooley Wastewater & Environmental Consulting Limited | Professional & scientific | 2023 |
| Door Motion Ltd | Manufacturing | 2024 |
| Dosa Takeaway Ltd | Hospitality | 2024 |
| Down to Earth Landscapes Services Limited | Administrative & support | 2025 |
| DPM Projects Ltd | Construction | 2024 |
| DR Mhairi Scally Robertson Psychology Ltd | Health & social care | 2025 |
| Dragongirl Bookkeeping LLP | | 2010 |
| Dramaking Ltd. | Wholesale & retail | 2024 |
| Dream Magic Superfood Powders Ltd | Wholesale & retail | 2024 |
| DRS Motorsport Ltd | Wholesale & retail | 2020 |
| Drty Holdings Ltd | Extraterritorial | 2024 |
| Drumcross Properties Ltd | Real estate | 2025 |
| Dsdk Holdings Ltd | Wholesale & retail | 2026 |
| Dsmo Services Ltd | Other services | 2018 |
| Duggan Mortgage Solutions Ltd | Financial services | 2024 |
| Dunnet Land Developments Ltd | Real estate | 2026 |
| DXB Property Limited | Real estate | 2022 |
| Dynamic Bodywork Academy Ltd | Education | 2014 |
| E&M Investments Limited | Real estate | 2024 |
| E&M Lavender Property Ltd | Real estate | 2024 |
| E. L Toner Consultancy Services Limited | Professional & scientific | 2022 |
| E.L.K Travel Ltd | Transport & logistics | 2018 |
| Eagle Bags Ltd | Transport & logistics | 2023 |
| Eagle Couriers (Holdings) Limited | Transport & logistics | 2005 |
| Eagle Couriers (Scotland) Limited | Transport & logistics | 1985 |
| Eagle Logistics Group Ltd | Transport & logistics | 2020 |
| Eastcoastcoffee Limited | Manufacturing | 2019 |
| Easy Street Food Ltd | Hospitality | 2024 |
| Easycore Ltd | Construction | 2022 |
| Eatalian Cultura Limited | Hospitality | 2025 |
| EC Learning Solutions Limited | Information & communication | 2023 |
| Echtrae Endeavour Enterprise Ltd | Financial services | 2026 |
| Eco Applications Ltd | Information & communication | 2021 |
| Eco Bride Studio Ltd | Wholesale & retail | 2024 |
| Eco Web Works Limited | Information & communication | 2025 |
| Econeng Limited | Professional & scientific | 1997 |
| Ecopower Solutions Ltd | Construction | 2020 |
| Ecotec Energy Ltd | Construction | 2012 |
| ED Private Tours Ltd | Administrative & support | 2026 |
| Ed&Eu Ltd | Information & communication | 2025 |
| EDB Digital Ltd | Professional & scientific | 2022 |
| Edebob Global Services Limited | Professional & scientific | 2025 |
| Eden Leisure Letting Ltd | Real estate | 2025 |
| Eden S Talent Limited | Professional & scientific | 2025 |
| Edencroft Equestrian Centre Ltd | Arts & recreation | 2022 |
| Edinburgh City Private Hire Holdings Limited | Professional & scientific | 2019 |
| Edinburgh City Private Hire Ltd. | Transport & logistics | 2007 |
| Edinburgh Fireworks Store Ltd | Administrative & support | 2010 |
| Edinburgh Painting Services Ltd | Construction | 2025 |
| Edinburgh Property Developers Ltd | Construction | 2022 |
| Edinburgh Winter Festivals Ltd | Arts & recreation | 2024 |
| Egerton House Developments Limited | Hospitality | 2017 |
| EH48 Distribution Ltd | Wholesale & retail | 2018 |
| EH48 Wash Ltd | Other services | 2025 |
| Ehsaas Care Ltd | Health & social care | 2018 |
| EIGHT16 Investments Limited | Financial services | 2025 |
| Ejaz School of Motoring Ltd | Education | 2021 |
| Ekiv Logistics Limited | Transport & logistics | 2025 |
| Eldex Consultancy Limited | Professional & scientific | 2013 |
| Electrosent Corporation Ltd | Information & communication | 2008 |
| Elevated Leaders Ltd. | Professional & scientific | 2013 |
| Elite Garage Services (Scotland) Ltd | Wholesale & retail | 2012 |
| Elite PA Solutions Ltd | Administrative & support | 2019 |
| Elite TAN Ltd | Other services | 2023 |
| Elite Vehicle Transport Scotland Limited | Transport & logistics | 2021 |
| Elizabethan Ltd | Hospitality | 2016 |
| Elmbank Property Renovations Ltd | Real estate | 2026 |
| Elrich Holdings Ltd | Real estate | 2025 |
| Elshani Limited | Hospitality | 2020 |
| Elshani Properties Limited | Real estate | 2022 |
| Elyt Commercial Properties Ltd | Real estate | 2019 |
| Ember Intelligence Ltd | Information & communication | 2025 |
| Emison Gases Limited | Administrative & support | 2022 |
| Emms Choice Limited | Professional & scientific | 2017 |
| Endlessdetailing.Co Ltd | Administrative & support | 2026 |
| Engage Proposals Ltd | Information & communication | 2023 |
| Enhance Aesthetics by Louise Ltd | Other services | 2026 |
| Enlin Limited | Hospitality | 2023 |
| Environmental Filtration Specialists Limited | Professional & scientific | 2016 |
| Envirotech Drainage Solutions Ltd | Water & waste | 2025 |
| Envirotecscotland Limited | Construction | 2024 |
| Epic Look Ltd | Health & social care | 2024 |
| Epic Services Group Ltd | Construction | 2018 |