| Diamond Shine Domestic Cleaning & Housekeeping Limited | Administrative & support | 2025 |
| Digi Global Ltd | Construction | 2023 |
| Digital Dragons IT Ltd | Information & communication | 2020 |
| DIGITAL347 Ltd | Professional & scientific | 2024 |
| Digitaltechno Ltd | Wholesale & retail | 2015 |
| Discount Wholesale and Distribution Limited | Real estate | 2017 |
| Distribution Forward Limited | Other services | 2019 |
| DIT Hartery & Co. Ltd | Hospitality | 2026 |
| Dixie Electrical Limited | Construction | 2024 |
| DJ & RA Griffiths Limited | Wholesale & retail | 2011 |
| Djange LLP | | 2009 |
| DJM Personal Training Ltd | Other services | 2013 |
| DM Care Trading AS the Care Revolution Limited | Professional & scientific | 2016 |
| DMG Lifts Ltd | Construction | 2020 |
| DMU Wales Limited | Professional & scientific | 2022 |
| DN8 Productions Ltd | Information & communication | 2025 |
| DNS Property Development Limited | Construction | 2026 |
| Dockview RD Management Ltd | Real estate | 2026 |
| Doctor Bobs Balloons Ltd | Wholesale & retail | 2013 |
| Donate for Research Initiative | Professional & scientific | 2012 |
| Donkey and GEE Ltd | Arts & recreation | 2022 |
| Door Maintenance Co. Limited | Manufacturing | 1984 |
| Door Maintenance Group Limited | Manufacturing | 2009 |
| Dorian Davies Consulting Limited | Professional & scientific | 2022 |
| Dorwyn Vale Properties Ltd | Real estate | 2025 |
| DOW Silicones UK Limited | Manufacturing | 1950 |
| Down 2 Earth 2 Ltd | Administrative & support | 2010 |
| Down at the Dales Limited | Real estate | 2023 |
| DR Glam Aesthetics Ltd | Health & social care | 2024 |
| DR Rosie Thomas Ltd | Health & social care | 2023 |
| Dragon Access Ltd | Extraterritorial | 2020 |
| Dragon Storage (Barry) Limited | Transport & logistics | 2021 |
| DRAGONS23 Limited | Education | 2023 |
| Dream Bathroom Solutions LLP | | 2024 |
| Dream Pizza Ltd | Hospitality | 2024 |
| Dreamfire-Uk Ltd | Information & communication | 2025 |
| Drew Way Consultants Ltd | Construction | 2013 |
| DSS DOM Scaffolding Services Ltd | Construction | 2018 |
| Dust Chain Ltd | Information & communication | 2025 |
| DW Accountancy Services Limited | Professional & scientific | 2020 |
| DW Plumbing & GAS Limited | Construction | 2021 |
| DW Plumbing Limited | Construction | 2017 |
| DWH Mayflower Ltd | Hospitality | 2023 |
| Dynamic Creative Group Ltd | Manufacturing | 2020 |
| Dynamite Cabs Ltd | Transport & logistics | 2025 |
| E R P Sandhu Ltd | Real estate | 2020 |
| E&E Platform Lifts Limited | Professional & scientific | 2022 |
| E, C & I Services Ltd | Administrative & support | 2018 |
| E-Tec Electrics Ltd | Information & communication | 2006 |
| E.H.Radcliffe & SON Limited | Agriculture & fishing | 1961 |
| EAA Homes Limited | Hospitality | 2019 |
| Eaam Foods Limited | Administrative & support | 2025 |
| Eastside Air Conditioning Ltd | Construction | 2019 |
| Eco Heating Limited | Construction | 2005 |
| Eco-Sol Limited | Manufacturing | 2007 |
| Ecommercemom Ltd | Wholesale & retail | 2021 |
| Ecube Solutions Limited | Water & waste | 2011 |
| Edec Construction Limited | Professional & scientific | 2019 |
| Edgeway Motors Limited | Wholesale & retail | 2026 |
| Edson Print (Services) Limited | Manufacturing | 1993 |
| Eduvision Ltd | Education | 2023 |
| Egora Ltd | Wholesale & retail | 2024 |
| ELECTRICIANS247.CO.UK. Ltd | Construction | 2025 |
| Electro Galvanising Limited | Real estate | 1955 |
| Elements Studios Design Ltd | Manufacturing | 2025 |
| Elevate Nutrition Ltd | Other services | 2022 |
| Elevated Training & Consultancy Services Ltd | Professional & scientific | 2024 |
| Elite Double Glazing Repairs Ltd. | Construction | 2024 |
| Elite International Student Consultancy Services Ltd | Administrative & support | 2011 |
| Elite Vehicle Support Services Limited | Wholesale & retail | 2026 |
| Ellen Williams Ltd | Real estate | 2010 |
| Elpis Logistics Ltd | Transport & logistics | 2020 |
| ELV in Digital Limited | Professional & scientific | 2014 |
| Ember Hour Ltd | Manufacturing | 2025 |
| EMBERST8 Limited | Manufacturing | 2026 |
| Emla Property Management Ltd | Administrative & support | 2012 |
| Empress Piercing Ltd | Wholesale & retail | 2021 |
| Enaid Accountancy Ltd | Professional & scientific | 2016 |
| Enchanted Bullies Limited | Agriculture & fishing | 2019 |
| Endura Projects Ltd | Construction | 2021 |
| Energy for Life Ltd. | Energy | 2011 |
| Energy Renewable Solutions Ltd | Construction | 2023 |
| Energyproelectrical Limited | Construction | 2024 |
| Enguard Ltd | Professional & scientific | 2015 |
| Enqusa Limited | Construction | 2025 |
| Envieux Apparel Ltd | Manufacturing | 2022 |
| Envol Commerce Ltd | Wholesale & retail | 2024 |
| Epilepsy Newcastle Ltd | Professional & scientific | 2023 |
| Epilogue Event Styling Floral Design Ltd | Wholesale & retail | 2019 |
| Epitom Concepts UK Ltd | Professional & scientific | 2023 |
| ERS Academy Ltd | Education | 2024 |
| ERS M&E Group Ltd | Financial services | 2024 |
| Esonic Neuron Delivery Ltd | Manufacturing | 2026 |
| Estate Planning Consultants Limited | Professional & scientific | 2016 |
| Estate Planning Partnership Limited | Professional & scientific | 2021 |
| Esterkin Developments Limited | Real estate | 1997 |
| Esterkin Holdings Limited | Financial services | 2018 |
| Esterkin Properties Limited | Real estate | 1999 |
| Estrella RTM Company Limited | Households | 2024 |
| Ethical Waste Ltd | Other services | 2025 |