| Barnsley Bolt and NUT Limited | Manufacturing | 2024 |
| Barnsley Brickwork Academy Limited | Construction | 2020 |
| Barnsley Brickwork Holdings Limited | Financial services | 2021 |
| Barnsley Brickwork Ltd | Construction | 2005 |
| Barnsley Business and Innovation Centre Limited | Other services | 1987 |
| Barnsley Card Ltd | Financial services | 2016 |
| Barnsley Civic Enterprise Limited | Arts & recreation | 2006 |
| Barnsley Civic Trading Limited | Real estate | 2022 |
| Barnsley Cleaning Services Ltd | Administrative & support | 2015 |
| Barnsley College Development Company Limited | Construction | 2009 |
| Barnsley Commercial Sales Ltd | Wholesale & retail | 2005 |
| Barnsley Community and Voluntary Services | Administrative & support | 2018 |
| Barnsley Community Media CIC | Manufacturing | 2025 |
| Barnsley Contract Flooring Ltd | Construction | 2026 |
| Barnsley Delicious Limited | Hospitality | 2026 |
| Barnsley Diner Limited | Hospitality | 2022 |
| Barnsley Disability Services Limited | Health & social care | 2002 |
| Barnsley Driving School Limited | Education | 2026 |
| Barnsley Eats Ltd | Information & communication | 2021 |
| Barnsley Electrical Supplies Limited | Wholesale & retail | 2009 |
| Barnsley Enterprises Health Limited | Health & social care | 2024 |
| Barnsley Enterprises Ltd | Wholesale & retail | 2012 |
| Barnsley ERP Ltd | Extraterritorial | 2026 |
| Barnsley European Market Limited | Wholesale & retail | 2026 |
| Barnsley Events and Catering Limited | Hospitality | 2025 |
| Barnsley Facilities Services Limited | Health & social care | 2012 |
| Barnsley Glass (2015) Limited | Manufacturing | 2015 |
| Barnsley Golf Club Ltd | Arts & recreation | 2011 |
| Barnsley Gymnastics Club Limited | Arts & recreation | 2008 |
| Barnsley Health Club Investments Holdings Limited | Professional & scientific | 2001 |
| Barnsley Health Club Investments Limited | Real estate | 1998 |
| Barnsley Healthcare Federation (BHF) CIC | Health & social care | 2015 |
| Barnsley Healthcare Federation CIC | Health & social care | 2016 |
| Barnsley Hospice Trading Limited | Wholesale & retail | 2010 |
| Barnsley Hot Tubs Limited | Wholesale & retail | 2014 |
| Barnsley Hypnosis & Counselling Limited | Health & social care | 2017 |
| Barnsley Independent Alzheimer's and Dementia Support (Biads) | Health & social care | 2010 |
| Barnsley Kebab Ltd | Hospitality | 2024 |
| Barnsley Live CIC | Arts & recreation | 2024 |
| Barnsley Local Medical Committee Limited | Professional & scientific | 2024 |
| Barnsley Lock & Safe Co. Limited | Wholesale & retail | 1992 |
| Barnsley Maintenance Ltd | Extraterritorial | 2022 |
| Barnsley Mini Skips Limited | Other services | 2021 |
| Barnsley Mortgages Ltd | Financial services | 2024 |
| Barnsley New Hong Kong Limited | Hospitality | 2023 |
| Barnsley Optical Limited | Administrative & support | 2008 |
| Barnsley Padel Club Limited | Arts & recreation | 2025 |
| Barnsley Phoenix Ltd | Hospitality | 2026 |
| Barnsley Powerwashing Limited | Construction | 2020 |
| Barnsley Premier Leisure | Arts & recreation | 1999 |
| Barnsley Premier Leisure Trading Limited | Hospitality | 1999 |
| Barnsley Rags Bags & Shoes Ltd | Wholesale & retail | 2012 |
| Barnsley Recovery Connections Ltd | Health & social care | 2024 |
| Barnsley Recruitment Services Ltd | Administrative & support | 2024 |
| Barnsley Refrigeration Services Limited | Manufacturing | 1996 |
| Barnsley Rufc and Supporters' Group Limited | Hospitality | 2016 |
| Barnsley Rugby Union Football Club Limited | Arts & recreation | 2012 |
| Barnsley Self Drive Hire Ltd | Administrative & support | 2003 |
| Barnsley Strafford Ltd | Real estate | 2013 |
| Barnsley Taxis Ltd | Transport & logistics | 2023 |
| Barnsley Theatre Trust (1998) Limited | Arts & recreation | 1993 |
| Barnsley Timber Merchants (Holdings) Limited | Financial services | 2017 |
| Barnsley Timber Merchants Limited | Wholesale & retail | 1986 |
| Barnsley Trials Club (Adult/Youth) Ltd | Other services | 2008 |
| Barnsley Tuition LLP | | 2026 |
| Barnsley Tyre Centre Ltd | Wholesale & retail | 2012 |
| Barnsley Van Hire Ltd | Administrative & support | 2006 |
| Barnsley Victoria Quarter Group Ltd | Real estate | 2025 |
| Barnsley Waste Disposal Limited | Water & waste | 2006 |
| Barnsley Waste Removals Limited | Extraterritorial | 2021 |
| Barnsley Women's Football Club Limited | Arts & recreation | 2018 |
| Barnsley Women's Football ETC Limited | Arts & recreation | 2022 |
| Barnsley Young Mens Christian Association (Incorporated) | Education | 1908 |
| Barnsley Youth Zone Ltd | Health & social care | 2023 |
| Barrett Earthworks Limited | Construction | 2018 |
| Barry Henderson Ltd | Construction | 2015 |
| Bars & PUB Management (Yorkshire) Ltd | Hospitality | 2026 |
| Bartec Auto ID Holdings Limited | Administrative & support | 2022 |
| Bartec Auto ID Limited | Administrative & support | 1992 |
| Bartec Municipal Technologies Holdings Limited | Manufacturing | 2022 |
| Bartec Municipal Technologies Limited | Information & communication | 2017 |
| Base Geotechnical Ltd | Professional & scientific | 2014 |
| Basicgrand Limited | Wholesale & retail | 1993 |
| BAT Head Records Ltd | Information & communication | 2007 |
| Battery Group Limited | Wholesale & retail | 2019 |
| Battery Logistics Ltd | Transport & logistics | 2026 |
| BAW Creative Ltd | Professional & scientific | 2022 |
| BAW Estates Limited | Real estate | 2003 |
| Bawtry Park Ltd | Extraterritorial | 2016 |
| Bayla Property Limited | Real estate | 2025 |
| BB Active Recovery Ltd | Health & social care | 2025 |
| BB Automotive Ltd | Wholesale & retail | 2026 |
| BBE Events Ltd | Arts & recreation | 2019 |
| Bbpackaging Ltd | Wholesale & retail | 2022 |
| BBT Transport Limited | Transport & logistics | 2018 |
| BC Collaborative Limited | Professional & scientific | 2023 |
| BCD Estates Limited | Real estate | 2023 |
| BCD Trans Ltd | Transport & logistics | 2016 |
| BCO Digital Ltd | Information & communication | 2025 |
| BCS Management Consultants Ltd | Professional & scientific | 2002 |