| Empulse Test Systems (UK) Limited | Manufacturing | 2021 |
| Endless Engine Limited | Information & communication | 2023 |
| Engineering Analysis Ltd | Professional & scientific | 2020 |
| Engineering Ingenuity Ltd | Professional & scientific | 2004 |
| English Language Matters Limited | Arts & recreation | 2005 |
| Enliven Limited | Extraterritorial | 1998 |
| Ensec-Solutions Ltd | Information & communication | 2023 |
| Ensemble Europe Limited | Manufacturing | 2010 |
| Enterpex Limited | Wholesale & retail | 2019 |
| Enterprise a Team Ltd | Wholesale & retail | 2018 |
| Entrepose Mills Scaffolding Limited | Administrative & support | 2001 |
| Envision Assets Inc Limited | Information & communication | 2025 |
| EP Gym Limited | Arts & recreation | 2015 |
| Ephemeral Limited | Professional & scientific | 2012 |
| Epicyclism Limited | Information & communication | 2024 |
| EPIK8 Limited | Professional & scientific | 2019 |
| Equestrian Theatre Limited | Arts & recreation | 2017 |
| Equilymph Ltd | Agriculture & fishing | 2013 |
| Equipe Geosolutions Limited | Administrative & support | 2009 |
| Equipe Training Limited | Construction | 2008 |
| ES Topsport Limited | Arts & recreation | 2003 |
| ESB Transport Limited | Transport & logistics | 2019 |
| ESG UK Mvna Limited | Information & communication | 2023 |
| Esim Go Limited | Information & communication | 2020 |
| Eslend Dinner Limited | Hospitality | 2020 |
| Essque Ltd | Information & communication | 2026 |
| Etcc Associates Limited | Professional & scientific | 2011 |
| Etchforge Ltd | Professional & scientific | 2025 |
| Etem Servicios Ltd | Professional & scientific | 2019 |
| Ethics Intelligence Ltd | Professional & scientific | 2025 |
| Etté Athletica Ltd | Manufacturing | 2022 |
| Euro UK Investments Limited | Construction | 2021 |
| Eurolead Services Holdings Limited | Extraterritorial | 2000 |
| European Supermarket Brands Limited | Extraterritorial | 1996 |
| Euroquip Automotive Limited | Manufacturing | 1984 |
| Euthis (UK) Limited | Wholesale & retail | 2026 |
| EVA Recruitment Ltd | Administrative & support | 2019 |
| Event Tech World Ltd | Information & communication | 2024 |
| Events Media UK Limited | Information & communication | 2020 |
| Evergreen Brides Ltd | Wholesale & retail | 2019 |
| Evergreen Entertainment Limited | Information & communication | 2009 |
| Evergreen Productions Limited | Information & communication | 2011 |
| Every Road Mobile Tyres Ltd | Wholesale & retail | 2026 |
| Everybody's Stuff Community Shop & Hub CIC | Education | 2024 |
| Evie and OX Limited | Wholesale & retail | 2022 |
| Evolution Services Europe Limited | Arts & recreation | 2006 |
| Evolve Church Academy Trust | Education | 2013 |
| Exact Air Ltd | Energy | 2000 |
| Excalibur Publishing Limited | Information & communication | 2003 |
| Excursions Direct Ltd | Administrative & support | 2023 |
| Expansia Informatics Ltd | Information & communication | 2012 |
| Expansive Ltd | Information & communication | 2019 |
| Expo Joinery LLP | | 2013 |
| Expolitus UK Ltd | Wholesale & retail | 2025 |
| Expressions Jewellery Limited | Wholesale & retail | 2003 |
| Extera Limited | Information & communication | 2001 |
| EYA Properties Ltd | Real estate | 2022 |
| EYE4QUALITY Limited | Information & communication | 2011 |
| Eyewitness Journeys Limited | Administrative & support | 2009 |
| Ez-Roofing Ltd | Construction | 2022 |
| F N Pile and Sons Ltd | Agriculture & fishing | 2020 |
| F3RDIB Ltd | Other services | 2022 |
| FA Global Enterprises Ltd | Wholesale & retail | 2025 |
| Fabio Logistics Ltd | Transport & logistics | 2018 |
| Fable Interiors Limited | Professional & scientific | 2014 |
| Factory Price Furniture Ltd | Wholesale & retail | 2020 |
| Fahertys Ltd | Hospitality | 2025 |
| Faircroft Cleaning Co. Limited | Administrative & support | 2022 |
| Fairfax Store Ltd | Wholesale & retail | 2023 |
| Fairfield Academic Support Limited | Education | 2022 |
| Fairfield Park Management (NO.1) Limited | Households | 1974 |
| Fairfield Supplies Limited | Wholesale & retail | 2013 |
| Fairway Express Ltd | Wholesale & retail | 2022 |
| Falcon Carriage Company Ltd | Transport & logistics | 2026 |
| Family Solutions Limited | Health & social care | 2024 |
| Fams Complex Care Limited | Administrative & support | 2022 |
| Fancare Limited | Professional & scientific | 2019 |
| Fancy UK Storage Ltd | Wholesale & retail | 2025 |
| Faraonu Ltd | Transport & logistics | 2025 |
| Farnreal Limited | Real estate | 2005 |
| Farrington Travel Co Ltd | Information & communication | 2025 |
| Farside Studio Ltd | Professional & scientific | 2024 |
| Farthinghoe Fine Wine Limited | Wholesale & retail | 1973 |
| Fasma Ltd | Professional & scientific | 2021 |
| Fast Track Cars Banbury Limited | Transport & logistics | 2025 |
| Fastest Gazelle Ltd | Hospitality | 2025 |
| Fastwall Limited | Construction | 2017 |
| FAT Jacks Ltd | Hospitality | 2021 |
| FAT Panda First AID Training Ltd | Education | 2018 |
| Fatdog Leisure Limited | Wholesale & retail | 2015 |
| Fatjon Properties Ltd | Real estate | 2025 |
| Fayrewood Ecology Ltd | Professional & scientific | 2025 |
| Fccj Ltd | Construction | 2025 |
| FCS Retail Ltd | Wholesale & retail | 2025 |
| FDA Group Ltd | Information & communication | 2019 |
| FE & UL Limited | Real estate | 2019 |
| Fearless Frog Films Ltd | Information & communication | 2019 |
| Featherstonehaugh Limited | Professional & scientific | 2015 |
| Feldon Herbs Ltd | Agriculture & fishing | 2004 |
| Feldon Maintenance Ltd | Other services | 2019 |