| Search Optimisation Ltd | Information & communication | 2004 |
| Secret Garden Events Ltd | Hospitality | 2025 |
| Service Accom Ltd | Hospitality | 2023 |
| Seventeen TEN Ltd | Professional & scientific | 2017 |
| SFB Contract Services Limited | Information & communication | 1992 |
| Sfco Ltd | Hospitality | 2021 |
| SFT Estates Limited | Professional & scientific | 2018 |
| Shakewell Limited | Wholesale & retail | 2021 |
| Shape Design Contracts Limited | Wholesale & retail | 1970 |
| Sheldon Antiques & Jewellery Ltd | Wholesale & retail | 2026 |
| SHG Catering Ltd | Hospitality | 2020 |
| Shine London Limited | Administrative & support | 2017 |
| Shutts Farm Machinery Ltd | Wholesale & retail | 2013 |
| Shutts Farm Services Ltd | Agriculture & fishing | 2009 |
| Sidney H. Noon Limited | Agriculture & fishing | 1948 |
| Signature Property Partners Ltd | Real estate | 2023 |
| Signpost Medical Writing Limited | Professional & scientific | 2019 |
| Simnia Ltd | Information & communication | 2025 |
| Simpson Electrical (Bakewell) Limited | Construction | 2023 |
| Simpstones Estates Ltd | Real estate | 2025 |
| Slaters Chimney Services Limited | Administrative & support | 2023 |
| Sma&I Ltd | Professional & scientific | 2026 |
| Smaxtec Limited | Wholesale & retail | 2020 |
| Smith's Island Car Park Limited | Administrative & support | 2013 |
| Smiths Runners Limited | Manufacturing | 1931 |
| SMW Coaching Limited | Professional & scientific | 2024 |
| SNP PVT Ltd | Transport & logistics | 2016 |
| SO Chiropractic Bedford Limited | Health & social care | 2013 |
| SO Chiropractic Limited | Health & social care | 2006 |
| SO Collective Living Ltd | Real estate | 2025 |
| Spear Financial Limited | Financial services | 2008 |
| Spectrum-Tec Ltd | Agriculture & fishing | 2026 |
| Spion KOP Care Home Limited | Health & social care | 2010 |
| SPJ Developments Limited | Construction | 2019 |
| Spooner Advisory Services Limited | Professional & scientific | 2019 |
| Sports Centre Ltd | Wholesale & retail | 2023 |
| Springboard Supported Accommodation Limited | Health & social care | 2025 |
| Springfield Leisure Limited | Manufacturing | 2020 |
| Sprintersvale Limited | Real estate | 2002 |
| ST. Olaf's Management Company Limited | Real estate | 2004 |
| ST2 Limited | Real estate | 2017 |
| Standard Recycling Limited | Other services | 2020 |
| Stantongrove Properties Ltd | Real estate | 2025 |
| Steel Cost Management Ltd | Professional & scientific | 2014 |
| Steelworks Recording Company Limited | Information & communication | 2015 |
| Steves Tyres & Wheels Limited | Wholesale & retail | 2012 |
| Stokes Property Services Limited | Hospitality | 2015 |
| Stonecrete Cleaning Services Limited | Administrative & support | 2015 |
| Stonehouse Fine Wines Limited | Professional & scientific | 1986 |
| Storage Storage Ltd | Other services | 2020 |
| Storm Access Limited | Other services | 2019 |
| Stuart Allen Agricultural Contracting Limited | Agriculture & fishing | 2003 |
| Stuart Berryman Limited | Administrative & support | 2014 |
| Stuart Britland Advisory Services Ltd. | Professional & scientific | 2016 |
| Studioboo Limited | Manufacturing | 2003 |
| Stunning Ltd | Other services | 2018 |
| Suttons Tax Limited | Professional & scientific | 2017 |
| SW3 (UK) Limited | Manufacturing | 2012 |
| Swabes Ltd | Professional & scientific | 2022 |
| Swarden Securities Limited | Financial services | 2007 |
| Sweet Genes Limited | Wholesale & retail | 2022 |
| Sweetlingo Ltd. | Extraterritorial | 2007 |
| Systemic Learning Limited | Professional & scientific | 2006 |
| T C Harrison 2000 Limited | Professional & scientific | 1987 |
| T C Harrison Ltd | Professional & scientific | 1936 |
| T Clarke & Sons Agri Plant Ltd | Wholesale & retail | 2025 |
| T&E Prime Properties Ltd | Construction | 2026 |
| T. C. Harrison Group Limited | Wholesale & retail | 1984 |
| T. C. Harrison JCB (North East) Limited | Professional & scientific | 1975 |
| T.C. Harrison (Commercial Leasing) Limited | Professional & scientific | 1956 |
| T.C. Harrison (Equipment Leasing) Limited | Professional & scientific | 1973 |
| T.C. Harrison (Industrial Leasing) Limited | Professional & scientific | 1967 |
| T.C. Harrison (Leasing Services) Limited | Professional & scientific | 1936 |
| T.C. Harrison (Leasing) Limited | Professional & scientific | 1965 |
| T.C. Harrison (Trucks) Limited | Professional & scientific | 1972 |
| T.C. Harrison (Trustees) Limited | Professional & scientific | 1975 |
| T.C. Harrison 1960 Limited | Administrative & support | 1960 |
| T.C. Harrison JCB Limited | Professional & scientific | 1975 |
| T.C.Harrison (Derby) Limited | Professional & scientific | 1928 |
| T.C.Harrison (Rotherham) Limited | Professional & scientific | 1925 |
| T.C.Harrison (Sheffield) Limited | Professional & scientific | 1971 |
| T.C.Harrison (Shepcote Lane) Limited | Professional & scientific | 1971 |
| T.C.Harrison(Newark)Limited | Professional & scientific | 1934 |
| Tabak's Big Top Workshop Limited | Administrative & support | 2026 |
| Tailpro Consulting Limited | Mining & quarrying | 2017 |
| Tansy Ashby Beauty Ltd | Other services | 2014 |
| Tarhill Holdings Ltd | Construction | 2022 |
| TAW Cars Limited | Transport & logistics | 2025 |
| Taylor Estate Management Limited | Real estate | 2024 |
| Teeceandfinch Ltd | Professional & scientific | 2020 |
| Test Faultless Limited | Manufacturing | 2013 |
| THE Ashford Arms Bakewell Ltd | Hospitality | 2023 |
| THE Ashover Light Railway Society | Education | 2009 |
| THE Bakewell Bakery Limited | Manufacturing | 2013 |
| THE Bakewell Preschool Playgroup Ltd | Health & social care | 2019 |
| THE Beagrie Group Ltd | Financial services | 2023 |
| THE Bect BUS Company Limited | Transport & logistics | 2021 |
| THE Bolton Abbey Estate Company Limited | Real estate | 2018 |
| THE Charleston Bakewell Ltd | Hospitality | 2024 |
| THE Cheesecake Factory Doncaster Ltd | Hospitality | 2019 |