| AYR Seafront Limited | Real estate | 2023 |
| AYR Self Storage Ltd | Transport & logistics | 2023 |
| AYR Skips Limited | Water & waste | 2023 |
| AYR Source One Ltd | Construction | 2025 |
| AYR Spinal Care Ltd | Health & social care | 2020 |
| AYR Sports Ltd | Wholesale & retail | 2024 |
| AYR Station Store Ltd | Wholesale & retail | 2025 |
| AYR Taxis Ltd | Transport & logistics | 2016 |
| AYR United Football Academy Limited | Arts & recreation | 2005 |
| AYR Vehicle Repairs Ltd | Wholesale & retail | 2023 |
| AYR Your Dirty Laundry Ltd | Other services | 2024 |
| Ayrfix Aviation Solutions Limited | Professional & scientific | 2008 |
| Ayrfix Limited | Other services | 2010 |
| Ayrluxstock Ltd | Wholesale & retail | 2025 |
| Ayroil Limited | Wholesale & retail | 2025 |
| Ayrshire & Glasgow Property Ltd | Real estate | 2025 |
| Ayrshire ALL Trades Ltd | Construction | 2024 |
| Ayrshire Alloy & Body Rescue Ltd | Wholesale & retail | 2026 |
| Ayrshire Bullion and Collectibles Ltd | Wholesale & retail | 2026 |
| Ayrshire Bulls Rugby Limited | Arts & recreation | 2022 |
| Ayrshire Canopy Cleaning Ltd | Administrative & support | 2020 |
| Ayrshire Car & Commercial Ltd | Wholesale & retail | 2019 |
| Ayrshire Car Traders Ltd | Wholesale & retail | 2014 |
| Ayrshire Classic Car Storage Ltd | Transport & logistics | 2021 |
| Ayrshire Classics Car Club Ltd | Other services | 2021 |
| Ayrshire Council on Alcohol the | Health & social care | 1986 |
| Ayrshire Dental Implant Centre Limited | Extraterritorial | 2011 |
| Ayrshire Engineering Solutions Ltd. | Manufacturing | 2017 |
| Ayrshire Entertainments Limited | Hospitality | 2014 |
| Ayrshire EYE Clinic Limited | Health & social care | 2012 |
| Ayrshire Family Law Ltd | Professional & scientific | 2025 |
| Ayrshire Foodie Ltd | Other services | 2025 |
| Ayrshire Garage Doors Limited | Wholesale & retail | 2006 |
| Ayrshire Gingerbeard Locksmiths Limited | Other services | 2020 |
| Ayrshire Healthcare Limited | Wholesale & retail | 2017 |
| Ayrshire Homestake Limited | Construction | 2003 |
| Ayrshire Independent BMW and Mini Specialists Ltd | Wholesale & retail | 2012 |
| Ayrshire Kitchens and Bedrooms Limited | Construction | 2025 |
| Ayrshire Letting Ltd | Real estate | 2024 |
| Ayrshire Magazine (Scotland) Ltd | Information & communication | 2022 |
| Ayrshire Marine Services Limited | Other services | 2017 |
| Ayrshire Medical Services Ltd | Other services | 2013 |
| Ayrshire Pizza Ltd | Hospitality | 2019 |
| Ayrshire Precision Engineering Limited | Manufacturing | 1970 |
| Ayrshire Precision Holdings Limited | Professional & scientific | 2018 |
| Ayrshire Property Holdings Ltd | Real estate | 2023 |
| Ayrshire Property Hub Ltd | Real estate | 2026 |
| Ayrshire Property Trading Ltd | Real estate | 2023 |
| Ayrshire Pubs Ltd | Hospitality | 2013 |
| Ayrshire Quail Ltd | Wholesale & retail | 2023 |
| Ayrshire Removals Ltd | Transport & logistics | 2021 |
| Ayrshire Roof Conversions Limited | Construction | 2017 |
| Ayrshire SC Ltd | Real estate | 2021 |
| Ayrshire Self-Service Launderette Ltd | Other services | 2019 |
| Ayrshire Sheds Ltd | Manufacturing | 2018 |
| Ayrshire Taxis Limited | Extraterritorial | 2015 |
| Ayrshire TLC Ltd | Professional & scientific | 2024 |
| Ayrshire Trade Frames Direct Ltd | Wholesale & retail | 2017 |
| Ayrshire Trade Frames Ltd | Construction | 2014 |
| Ayrshire Transport Limited | Transport & logistics | 2018 |
| Ayrshire Vehicle Rental Limited | Administrative & support | 2020 |
| Ayrshire Wedding Cars Limited | Administrative & support | 2016 |
| Ayrshire Will Writing Ltd | Administrative & support | 2021 |
| Azad Haque Holdings Limited | Real estate | 2024 |
| Azfc Ltd | Hospitality | 2025 |
| B & E Inns Limited | Hospitality | 2018 |
| B Designer Homes Ltd | Construction | 2020 |
| B Home Contracts Ltd | Construction | 2025 |
| B W Funeral Directors Ltd | Other services | 2013 |
| B&E Co. Ltd | Arts & recreation | 2024 |
| B. & L. Investments Limited | Real estate | 1989 |
| Baby to Teen CIC | Wholesale & retail | 2026 |
| Bailiot Ltd | Hospitality | 2024 |
| Baillie's Healthcare Limited | Wholesale & retail | 2017 |
| Balance Wellness Clinic Ltd | Health & social care | 2020 |
| Baldwin Corporate LP | | 2017 |
| Ballyblack Properties Limited | Real estate | 2012 |
| Balmoral Funeral Directors Glasgow Ltd | Other services | 2020 |
| Balmoral Funeral Directors Irvine Ltd | Other services | 2020 |
| Balmoral Funeral Directors Kilmarnock Ltd | Other services | 2020 |
| Bannockburn Property Limited | Administrative & support | 2025 |
| Bar Gallus Glasgow Ltd | Hospitality | 2019 |
| Bar Pazz Ltd | Hospitality | 2024 |
| Barbell Barbie Ltd | Other services | 2021 |
| Barochan Biomass Energy Limited | Other services | 2014 |
| Barochan Heathfield LLP | | 2010 |
| Barr Muir Property INS Ltd | Construction | 2016 |
| Barral UK Conferences Ltd | Administrative & support | 2011 |
| Barral UK Visceral Ltd | Education | 2011 |
| Barrmuir Construction Ltd | Construction | 2021 |
| Barrmuir Kitchen and Bathroom Scotland Ltd | Manufacturing | 2024 |
| Barro Ventures Ltd | Financial services | 2025 |
| Barry Curtis Personal Training Ltd | Other services | 2024 |
| Based Body Works Ltd | Wholesale & retail | 2026 |
| Basrai Ltd | Hospitality | 2007 |
| Basrai Restaurant (Blackpool) Limited | Hospitality | 2014 |
| Basrai Restaurant Limited | Hospitality | 2011 |
| Bath Heaven Ltd | Wholesale & retail | 2024 |
| Bath Street Trading Limited | Hospitality | 2019 |
| Bathurst Care Limited | Health & social care | 2019 |