| 1 Alba Cabs Limited | Transport & logistics | 2015 |
| 12 Bar Studios Ltd | Arts & recreation | 2015 |
| 163 Takeaway Limited | Hospitality | 2024 |
| 17POINT9 Limited | Real estate | 2023 |
| 19 AYR Ltd | Real estate | 2026 |
| 1ST Legal Limited | Professional & scientific | 2012 |
| 21J Stress Solutions Limited | Professional & scientific | 2014 |
| 247 Exterior Cleaning Ltd | Administrative & support | 2021 |
| 289 Limited | Construction | 2023 |
| 2ND Terrace Ltd | Wholesale & retail | 2026 |
| 360 Blades and Tower Training Limited | Education | 2024 |
| 360 Mobile Training Limited | Education | 2023 |
| 360 Safety & Engineering Limited | Manufacturing | 2013 |
| 360 Safety & Training Limited | Education | 2016 |
| 360 Safety Ltd | Professional & scientific | 2020 |
| 360 Workshop Services Limited | Manufacturing | 2026 |
| 3DI Group Ltd | Financial services | 2011 |
| 3NC Construction Ltd | Construction | 2025 |
| 4 Hire 4 Work Limited | Administrative & support | 2016 |
| 4YOU Hairdressing Ltd | Other services | 2022 |
| 5G Capital Limited | Real estate | 2021 |
| 7R Logistics Limited | Hospitality | 2019 |
| A & H Joiners Limited | Construction | 2014 |
| A & J Flooring (Scotland) Ltd | Wholesale & retail | 2022 |
| A & JT Investments Limited | Real estate | 2013 |
| A & L Simpson Ltd | Wholesale & retail | 2019 |
| A B C Intelligence Limited | Financial services | 2013 |
| A C White Solicitors LLP | | 2022 |
| A H T Properties Ltd | Extraterritorial | 2018 |
| A Hygienic Ltd | Health & social care | 2023 |
| A Mccandlish Agricultural Contractors Ltd | Professional & scientific | 2013 |
| A Mcfadzean Limited | Wholesale & retail | 2018 |
| A Mckie Builders Limited | Construction | 2019 |
| A Patrick Builders Ltd | Construction | 2010 |
| A Pet Hotel Ltd | Real estate | 2014 |
| A T Property Consulting Ltd | Administrative & support | 2016 |
| A T S Property Scotland Limited | Real estate | 2023 |
| A V Enterprises (Ayrshire) Ltd | Transport & logistics | 2019 |
| A&N Property Ayrshire Limited | Real estate | 2026 |
| A-Z Partners Ltd | Wholesale & retail | 2025 |
| A. Connolly & Buchanan Limited | Real estate | 1959 |
| A.C.E Supporting Services Limited | Health & social care | 2024 |
| A.M Taxis (Ayrshire) Ltd | Transport & logistics | 2019 |
| A.S.N.R. Holdings Limited | Financial services | 2010 |
| A.T. Hendry Architect Limited | Professional & scientific | 2022 |
| A1 Ironmongery Limited | Wholesale & retail | 2011 |
| Aadya's Builders Ltd | Construction | 2024 |
| Aadya's Homes Ltd | Construction | 2022 |
| AAM Properties & Contracts Ltd | Real estate | 2023 |
| Aarjas Ltd | Wholesale & retail | 2023 |
| Aarosh Limited | Arts & recreation | 2017 |
| AAT Projects and Repairs Limited | Professional & scientific | 2023 |
| AAT Safety Limited | Professional & scientific | 2020 |
| AAT Site Operational Support Limited | Professional & scientific | 2023 |
| AAT Wind Energy Limited | Professional & scientific | 2009 |
| AB Catering AYR Ltd | Hospitality | 2018 |
| AB Vision Financial Ltd | Financial services | 2014 |
| Abacus Plumbing & Heating (Ayrshire) Limited | Construction | 2006 |
| ABC 4D Limited | Health & social care | 2012 |
| ABC GRP Ltd | Administrative & support | 2018 |
| Abcsoftplayhire Ltd | Arts & recreation | 2025 |
| Able Decorators & Plastering Limited | Construction | 2018 |
| Absolute Event Management Limited | Administrative & support | 2002 |
| ABT CA Ltd | Real estate | 2019 |
| AC Fast Food (Newmains) Limited | Hospitality | 2024 |
| Acatch Environmental & Property Services Ltd | Other services | 2017 |
| ACC Joinery Limited | Construction | 2015 |
| Accelerate Accounting Ltd | Professional & scientific | 2024 |
| Accelerate Corporation Ltd. | Professional & scientific | 2001 |
| Accurate Fire and Security Systems Limited | Manufacturing | 2014 |
| Ace Animal Care Ltd | Professional & scientific | 2022 |
| Ace Farming Ltd | Other services | 2018 |
| Ace Plastering Ayrshire Ltd | Construction | 2025 |
| Ace Tyre Services Ltd | Wholesale & retail | 2015 |
| Ace Tyres Wholesale UK Limited | Wholesale & retail | 2024 |
| ACH Enterprise Limited | Education | 2025 |
| Achievement Shawlands Ltd | Arts & recreation | 2019 |
| ACL (Scotland) Limited | Construction | 2017 |
| Acorn Shutters and Blinds Ltd | Wholesale & retail | 2010 |
| Active Chiropractic Care Limited | Health & social care | 2019 |
| Activecbt Ltd | Professional & scientific | 2021 |
| Adam Mclaughlin Joinery Limited | Construction | 2004 |
| ADM Garden Buildings Ltd | Wholesale & retail | 2025 |
| Adnr Ltd | Professional & scientific | 2015 |
| Adored Joinery Ltd | Construction | 2021 |
| ADR Aesthetique Ltd | Other services | 2022 |
| Advanced Integrated Solutions Ltd | Construction | 2010 |
| Advertisingworks Scotland Ltd. | Professional & scientific | 2002 |
| Aegirfish Limited | Wholesale & retail | 1995 |
| Aero Leap Consultancy Ltd | Professional & scientific | 2025 |
| Aeroshark Ltd | Professional & scientific | 2022 |
| AES [Equipment Cleaning] Limited | Manufacturing | 2009 |
| Aesthetics Ayrshire by Leah Ltd | Health & social care | 2026 |
| Aesthetics by Emma Ltd | Other services | 2024 |
| AF Roofing & Building Ltd | Construction | 2022 |
| AFK Property Development Ltd | Construction | 2018 |
| Afro Foods and Butchery Ltd | Wholesale & retail | 2025 |
| Afton Valley Joiners Ltd | Construction | 2025 |
| AGA01 Ltd | Hospitality | 2018 |
| Agga Holdings Ltd | Wholesale & retail | 2018 |