| Neizer Commercial Ltd | Wholesale & retail | 2026 |
| Nella Services Ltd | Hospitality | 2019 |
| Nelluri Tech Limited | Information & communication | 2015 |
| Nemo Bookkeeping Limited | Professional & scientific | 2019 |
| Nene Associates Ltd | Information & communication | 2016 |
| Neon Electrical (South) Ltd | Construction | 2022 |
| Neonatal Care Ltd | Other services | 2024 |
| Neosimuk Ltd | Health & social care | 2022 |
| Neptune Recruit Ltd | Health & social care | 2021 |
| Nerdware Ltd | Information & communication | 2020 |
| Nerwande Limited | Professional & scientific | 2024 |
| Nest Aylesbury Vale Limited | Health & social care | 2006 |
| Nestayra Limited | Real estate | 2025 |
| Netsearch International Limited | Administrative & support | 2000 |
| Nettlebed Insights Limited | Professional & scientific | 2023 |
| Network Stratigraphic Consulting Limited | Mining & quarrying | 1994 |
| Netzach Limited | Information & communication | 2005 |
| Neuino Tech Limited | Wholesale & retail | 2021 |
| Neuro Resilience CIC | Education | 2026 |
| Neuro Speech Therapy Oxford Ltd | Health & social care | 2023 |
| Neuroflourish Ltd | Professional & scientific | 2017 |
| Neurowise Academy Ltd | Education | 2024 |
| Neutral Density Limited | Administrative & support | 2017 |
| Nevaki Asset Private Limited | Real estate | 2022 |
| New Ainsleys Limited | Professional & scientific | 2023 |
| New Aylesbury Tuck Ltd | Hospitality | 2025 |
| New Body Osteopathy Ltd | Health & social care | 2003 |
| New ERA Logistics Limited | Transport & logistics | 2012 |
| New ERA Lunar Innovations Ltd | Administrative & support | 2024 |
| New Gardener Ltd | Wholesale & retail | 2021 |
| New Green Services Limited | Professional & scientific | 2003 |
| New Homes Consulting Limited | Construction | 2009 |
| New Homes Customer Care Limited | Construction | 2003 |
| New Line Software Limited | Information & communication | 2016 |
| New Link (Southern) Limited | Wholesale & retail | 2018 |
| New Look Driveways Ltd | Construction | 2026 |
| New Road Flats (Moreton) Management Company Ltd | Households | 2024 |
| New Treasure House Ltd | Other services | 2023 |
| New Vision Support Limited | Administrative & support | 2025 |
| New York Deli (Aylesbury) Limited | Wholesale & retail | 2011 |
| New Zavia Ltd | Administrative & support | 2016 |
| New Zealand (Aylesbury) Ltd | Hospitality | 2012 |
| Newark Technology Solutions Limited | Information & communication | 2015 |
| Newbridge Motorsport International Limited | Arts & recreation | 2020 |
| Newfunapps Ltd | Wholesale & retail | 2025 |
| Newgen Heating Solutions Limited | Construction | 2022 |
| Newington Business Consulting Ltd | Administrative & support | 2025 |
| Newleigh Construction Ltd | Construction | 2022 |
| Newlinkit Solutions Ltd | Information & communication | 2018 |
| Newton Landscaping Ltd | Construction | 2019 |
| Nex-G Arti Ltd | Manufacturing | 2004 |
| Nexadeeptech Limited | Information & communication | 2026 |
| Nexagen Cricket Academy Ltd | Education | 2026 |
| Nexainsight Ltd | Professional & scientific | 2023 |
| Nexaone Limited | Administrative & support | 2024 |
| Nexim Healthcare Consultants Ltd | Information & communication | 2018 |
| Nexium Ltd | Information & communication | 2014 |
| Next Phase Academy Limited | Education | 2024 |
| Next Racing Generation Academy Ltd | Education | 2025 |
| Next-Door Mechanic Ltd | Administrative & support | 2025 |
| Nextdata Ltd | Information & communication | 2019 |
| Nexus CRS Ltd | Health & social care | 2025 |
| Nexusonomy Ltd | Information & communication | 2018 |
| NFL Landscapes Ltd | Administrative & support | 2020 |
| Ngage Business Services Limited | Professional & scientific | 2006 |
| Ngage Solutions Limited | Professional & scientific | 2000 |
| NGB Mortgage Consulting Ltd | Financial services | 2016 |
| Ngote Services Limited | Construction | 2025 |
| NGU Catering Ltd | Hospitality | 2024 |
| NGU Logistics Ltd | Transport & logistics | 2019 |
| NH Electrical Solutions Ltd | Construction | 2014 |
| NHB Consulting Limited | Professional & scientific | 2012 |
| NHT Plus Limited | Health & social care | 2013 |
| NHT Rath Ceilings & Partitions Limited | Construction | 2003 |
| NIA Music Limited | Arts & recreation | 2010 |
| Nials Consultancy Ltd | Professional & scientific | 2024 |
| Nials Ltd | Real estate | 2023 |
| Nicco Consultancy Ltd | Professional & scientific | 2023 |
| Niche Bargains Ltd | Wholesale & retail | 2026 |
| Niche Bars Limited | Administrative & support | 2014 |
| Nicholas James Design Limited | Construction | 2002 |
| Nicholas Movers Limited | Transport & logistics | 2024 |
| Nick Bicat Music Limited | Arts & recreation | 1998 |
| Nick Hughes Consulting Ltd | Administrative & support | 2007 |
| Nick Jugaru Construction Ltd | Construction | 2015 |
| Nick Lincoln Limited | Information & communication | 2019 |
| Nick MCC Limited | Health & social care | 2024 |
| Nicky Harrold Limited | Professional & scientific | 2015 |
| Nicl Limited | Real estate | 2024 |
| Nienco Limited | Information & communication | 2019 |
| Nigel Young Marketing Ltd | Professional & scientific | 1999 |
| Nigerian Community Association Aylesbury CIC | Professional & scientific | 2015 |
| Night and Day Limited | Administrative & support | 1993 |
| Night Watch Security Ltd | Other services | 2017 |
| Nikad Group Holdings Limited | Manufacturing | 1992 |
| Nikeni Ltd | Health & social care | 2012 |
| Nikeola Limited | Health & social care | 2024 |
| Nikodem Homes Ltd | Real estate | 2020 |
| Nimadal Limited | Hospitality | 2025 |
| Nina Petty Speech and Language Therapy Services Limited | Health & social care | 2013 |