| Siaway Auchterarder Limited | Hospitality | 2024 |
| Signet Water Limited | Professional & scientific | 2020 |
| Silverweave Marketing Limited | Real estate | 2006 |
| Single Malt Distiller Limited | Manufacturing | 2019 |
| SJ Anderson Holdings Limited | Real estate | 2013 |
| SJF Investments Ltd | Real estate | 2017 |
| Sklb Properties Ltd | Real estate | 2024 |
| Skymore Farm Limited | Agriculture & fishing | 2024 |
| Sofia Spmu Limited | Other services | 2023 |
| Speyborn Limited | Education | 2024 |
| Sprint Finish Studio Ltd | Wholesale & retail | 2025 |
| Square Away Stays Ltd | Real estate | 2024 |
| ST Mungo's Farms | | 2001 |
| St.Pierre Offshore Ltd | Other services | 2024 |
| Stag Developments (Scot) Limited | Construction | 2008 |
| Stewart Brothers Bathroom Collections Limited | Wholesale & retail | 2017 |
| Stone Faced Block Company Ltd | Manufacturing | 2009 |
| Strathallan Properties Ltd | Real estate | 2021 |
| Strathearn Consultancy Limited | Professional & scientific | 2018 |
| Strathearn Developments Limited | Real estate | 1980 |
| Strathearn Engineering Ltd. | Wholesale & retail | 2005 |
| Strathearn Leisure (Auchterarder) | | 1992 |
| Strathearn Luxury Homes Ltd | Real estate | 2025 |
| Stravaig Space Ltd | Real estate | 2025 |
| Studio K Architects Ltd | Professional & scientific | 2014 |
| Subzerocryolab Limited | Other services | 2025 |
| Sugar and Spice (2021) Ltd | Wholesale & retail | 2021 |
| Synco Properties Ltd | Households | 2023 |
| T&A Niblick Ltd | Hospitality | 2024 |
| Taggs Blackford Ltd | Administrative & support | 2026 |
| Tango's (Scotland) Ltd | Hospitality | 2019 |
| Taste of the Trossachs Ltd | Real estate | 2012 |
| Techguyjd Repairs Limited | Manufacturing | 2023 |
| THE Auchter Larder Ltd | Wholesale & retail | 2024 |
| THE David Donnachie Family Investment Company Limited | Professional & scientific | 2019 |
| THE Empath Chef Ltd | Information & communication | 2024 |
| THE Flower POD Ltd | Wholesale & retail | 2026 |
| THE Gentle Touch (Strathaven) Limited | Other services | 2000 |
| THE Glasgow Wine Club Ltd | Hospitality | 2024 |
| THE Highland Games Events Company Limited | Arts & recreation | 2025 |
| THE Highland Queen Scotch Whisky Company Ltd | Administrative & support | 2009 |
| THE Horse Shop Ltd | Wholesale & retail | 2025 |
| THE Pretoria Bar Crieff Ltd | Hospitality | 2018 |
| THE Strathearn Food Company Ltd | Other services | 2020 |
| THE TAN Bar (Auchterarder) Ltd | Other services | 2025 |
| THE Tippler Limited | Professional & scientific | 2016 |
| THE Warehouse Kitchen and Bathroom Company Limited | Manufacturing | 2019 |
| THE WEE Dairy Limited | Agriculture & fishing | 2023 |
| THE Wine Hall Ltd | Wholesale & retail | 2022 |
| TOM Drysdale Creative Studio Ltd | Professional & scientific | 2025 |
| Townhead Feeds Ltd | Wholesale & retail | 2016 |
| Tradeset Solutions Ltd | Construction | 2023 |
| Treaders Traders Ltd | Transport & logistics | 2023 |
| Triple F Consultants Limited | Professional & scientific | 2025 |
| Trls&Rrs Ltd | Professional & scientific | 2020 |
| Tubular Connections Ltd | Professional & scientific | 2015 |
| Tullibardine Leisure Ltd | Wholesale & retail | 2021 |
| Ukspba Ltd | Arts & recreation | 2025 |
| VS Tours Ltd | Administrative & support | 2026 |
| Wallace Collective Ltd | Wholesale & retail | 2025 |
| Water for Health Ltd | Wholesale & retail | 2006 |
| Wayne Smith Renovations Ltd | Construction | 2017 |
| WBP SCO C LLP | | 2024 |
| Well Finished Developments Ltd | Real estate | 2022 |
| West Mains Farm Ltd | Agriculture & fishing | 2025 |
| White Water Coaching & Counselling Ltd. | Professional & scientific | 2009 |
| William Lawson Plumbers Ltd | Construction | 2021 |
| WKC Painting & Decorating Ltd | Construction | 2024 |
| YEE 8 Ltd | Hospitality | 2012 |
| Zappar (Holding) Limited | Professional & scientific | 2011 |
| Zappar Limited | Information & communication | 2011 |
| Zielinski Associates Limited | Administrative & support | 2016 |