| Apollo Accounting Ltd | Professional & scientific | 2017 |
| Appcrowd Ltd | Information & communication | 2011 |
| Appletree Advisory Ltd | Professional & scientific | 2020 |
| Aqua Narrowboats (UK) Limited | Real estate | 2010 |
| Aqua Pro Pool & Spa Specialist Ltd | Administrative & support | 2023 |
| AR Business Supplies Ltd | Other services | 2018 |
| ARA Engineering Limited | Professional & scientific | 2016 |
| Aradyan Motors Limited | Wholesale & retail | 2020 |
| Arbormasters Ltd | Agriculture & fishing | 2002 |
| Arbour Walk Ltd | Financial services | 2025 |
| ARC Ceilings & Partitions Ltd | Construction | 2023 |
| Archer Fires Ltd | Manufacturing | 2023 |
| Archtop Joinery Limited | Manufacturing | 2012 |
| Arden Exclusive Travel Ltd | Transport & logistics | 2016 |
| ARK Impact Limited | Professional & scientific | 2024 |
| ARK Keystone Investments Ltd | Real estate | 2026 |
| ARK Safety Consultancy Ltd | Professional & scientific | 2025 |
| Arlen Education Ltd | Education | 2015 |
| Arme Holdings Limited | Financial services | 2024 |
| Arnold Betts Limited | Real estate | 2024 |
| Art Horizon Limited | Professional & scientific | 2021 |
| Artbourne Limited | Administrative & support | 2025 |
| Artful Resolve Designs Limited | Professional & scientific | 2023 |
| Artisan Creative Communities Ltd | Arts & recreation | 2022 |
| Artisan Creative Limited | Information & communication | 2008 |
| Artur Building Services Limited | Construction | 2026 |
| Ascend Access Limited | Professional & scientific | 2025 |
| Aseno Ltd | Professional & scientific | 2026 |
| Ashbourne Allotment Association Limited | Agriculture & fishing | 2011 |
| Ashbourne Arts Limited | Arts & recreation | 2002 |
| Ashbourne Biscuits Limited | Extraterritorial | 2006 |
| Ashbourne Brickwork Limited | Construction | 2014 |
| Ashbourne Community Transport | Transport & logistics | 2005 |
| Ashbourne Football Club Limited | Arts & recreation | 2018 |
| Ashbourne Golf Club Limited(The) | Arts & recreation | 1980 |
| Ashbourne Link Centre Ltd | Hospitality | 2024 |
| Ashbourne Metals Ltd | Wholesale & retail | 2016 |
| Ashbourne OBD Ltd | Wholesale & retail | 2015 |
| Ashbourne Quality Care Ltd | Administrative & support | 2016 |
| Ashbourne Quality Meats Limited | Financial services | 2018 |
| Ashbourne Refugee Community Support Group Limited | Health & social care | 2025 |
| Ashbourne Roofing Limited | Construction | 2007 |
| Ashbourne Rugby Union Football Club Limited | Arts & recreation | 2007 |
| Ashbourne Scaffolding Limited | Construction | 2013 |
| Ashbourne Shire Horse Society | Administrative & support | 2003 |
| Ashbourne Shoe Repairs Limited | Manufacturing | 2005 |
| Ashbourne Vapes Ltd | Wholesale & retail | 2025 |
| Ashby Steam Ltd | Arts & recreation | 2019 |
| Ashfen Aluminium Ltd | Manufacturing | 2026 |
| Ashwood Timber & Plastics Limited | Manufacturing | 2006 |
| ASK Systems UK Ltd | Professional & scientific | 2013 |
| ASP Manufacturing Ltd | Professional & scientific | 2025 |
| Association of Professional Shooting Instructors Ltd | Other services | 2017 |
| Atlow Winn Consulting Limited | Professional & scientific | 2012 |
| Aumdentgroup Ltd | Health & social care | 2025 |
| Austin Fitness Ltd | Arts & recreation | 2026 |
| Avalon Psychological Health Limited | Health & social care | 2015 |
| Avanti Goldsmiths Ltd | Manufacturing | 2022 |
| Avanti of Ashbourne Limited | Wholesale & retail | 2008 |
| Avela Aesthetics Limited | Health & social care | 2025 |
| B Pegg Haulage Ltd | Transport & logistics | 2016 |
| B.U.Placements Ltd | Education | 2020 |
| B.W Asset Holdings Limited | Real estate | 2017 |
| B.W Commercial Properties Ltd | Real estate | 2024 |
| B.W Engineering (UK) Ltd | Manufacturing | 2013 |
| Baas Wholesale Limited | Real estate | 2010 |
| Baby Swim School Ltd | Education | 2011 |
| Bailey Bears Sensory DEN CIC | Health & social care | 2023 |
| Bakes+Cakes Ltd | Hospitality | 2025 |
| Ballarz Ltd | Wholesale & retail | 2023 |
| Bandglade 11 Limited | Construction | 2007 |
| Bandglade Developments Limited | Construction | 1986 |
| Banjo Retail Limited | Wholesale & retail | 2005 |
| Bank House Associates Limited | Professional & scientific | 2007 |
| Barakuda Limited | Information & communication | 2008 |
| Barleymans Limited | Water & waste | 2003 |
| Bartof Limited | Administrative & support | 2006 |
| Basement Thai Restaurant Ltd | Hospitality | 2011 |
| Basis Classroom Limited | Education | 2021 |
| Basis Ramps Limited | Other services | 2012 |
| Basis Registration Limited | Other services | 1978 |
| Baskerville X-Ray Ltd | Professional & scientific | 2025 |
| Bateman Comfort Homes Ltd | Real estate | 2025 |
| Bayley's of Rosliston Limited | Agriculture & fishing | 2005 |
| Bayley’S Agri Ltd | Manufacturing | 2026 |
| Bayliss Autovents Limited | Extraterritorial | 1976 |
| Bayliss Commercial Holdings Limited | Real estate | 2010 |
| Bayliss Precision Components Limited | Manufacturing | 1969 |
| BCL Acquisitions Ltd | Manufacturing | 2017 |
| BE Human Partnership Ltd | Information & communication | 2025 |
| Beauchamp Business Park Management Company Limited | Real estate | 2023 |
| Becky Meadows Consulting Limited | Professional & scientific | 2007 |
| Beechenhill Artworks Limited | Professional & scientific | 1998 |
| Bellows Glamping and Events Ltd | Administrative & support | 2019 |
| Bellton Properties Limited | Real estate | 1959 |
| Belucci Ltd | Wholesale & retail | 2021 |
| Benedict MEP Ltd | Professional & scientific | 2025 |
| Bentley Bridge Management Company Limited | Real estate | 2009 |
| Bentleyfold Management Limited | Households | 1996 |
| Beobridge Farms Limited | Agriculture & fishing | 2025 |