| Vegas Barber Limited | Other services | 2020 |
| Vehicle One Stop Limited | Wholesale & retail | 2009 |
| Venture Accessories Ltd | Wholesale & retail | 2018 |
| Venture Flooring Limited | Construction | 2010 |
| Venture Vehicles Ltd | Wholesale & retail | 2017 |
| Verndale Systems Limited | Information & communication | 1989 |
| Vertex Consulting Limited | Information & communication | 2005 |
| Veso Ltd | Professional & scientific | 2007 |
| Veterinary Endoscopy Training Services Ltd | Professional & scientific | 2022 |
| Vianova UK Limited | Professional & scientific | 2004 |
| Viceroy 101 Limited | Professional & scientific | 2020 |
| Viceroy 102 Limited | Professional & scientific | 2020 |
| Viceroy 103 Limited | Professional & scientific | 2020 |
| Viceroy 2020 Limited | Professional & scientific | 2020 |
| Village Hospitality Ltd | Hospitality | 2023 |
| Village Taxis of Ashbourne Limited | Transport & logistics | 2018 |
| Vintage Thinking Ltd | Professional & scientific | 2020 |
| Violet Evelyn Ltd | Wholesale & retail | 2025 |
| Virgil Estates Ltd | Real estate | 2022 |
| Vital Training Services Limited | Administrative & support | 2004 |
| Vitesse Engineering Services Ltd | Professional & scientific | 2009 |
| Vitmedics Ltd | Health & social care | 2020 |
| Viva Hair & Beauty (Ashbourne) Ltd | Other services | 2015 |
| Vivello Solutions Ltd | Manufacturing | 2024 |
| Vivid Soul Ltd | Arts & recreation | 2023 |
| VJK Limited | Real estate | 2019 |
| Vsta EEE Consultancy Limited | Professional & scientific | 2020 |
| Vulcan Self Storage Limited | Real estate | 2020 |
| W & PE Gosling & SON Limited | Agriculture & fishing | 2008 |
| W Barker and Sons Limited | Transport & logistics | 2013 |
| W.H.Ratcliffe(Haulage)Limited | Transport & logistics | 1961 |
| Walbrook Coaching Limited | Professional & scientific | 2016 |
| Walker Farms Ltd | Agriculture & fishing | 2008 |
| Walkers of Ashbourne Limited | Transport & logistics | 1978 |
| Walkthrough Partners Limited | Administrative & support | 2025 |
| Walters Way Ltd | Real estate | 2023 |
| Water Applications Ltd | Construction | 2023 |
| Waterfield Property Ltd. | Construction | 2010 |
| Watkiss Thermalbreak Limited | Manufacturing | 1996 |
| Wayahead Training Limited | Education | 2015 |
| Wcfm Ltd | Health & social care | 2023 |
| Websearch Limited | Hospitality | 2011 |
| Wellcroft Grange Management Company Limited | Households | 2001 |
| Wellcroft Holiday Cottages Ltd | Hospitality | 2026 |
| Wellcroft Holiday Lets Ltd | Hospitality | 2026 |
| WES Developments Limited | Construction | 2025 |
| Wessex Capital Limited | Real estate | 2000 |
| West Viking Way Management Company Limited | Real estate | 2024 |
| WG Services (Derby) Limited | Administrative & support | 2021 |
| Wharf Property Limited | Real estate | 2026 |
| Whiskey FOX Limited | Professional & scientific | 2024 |
| White Barn Co. Ltd | Wholesale & retail | 2022 |
| White Peak Building Services Limited | Construction | 2017 |
| White Peak Cleaning Ltd | Administrative & support | 2012 |
| White Peak Dental Limited | Health & social care | 2013 |
| White Peak Plant Hire Limited | Administrative & support | 2025 |
| Whitehouse Construction Co. Limited | Construction | 1977 |
| Whitehouse P.L.P Holdings Limited | Financial services | 2017 |
| Whitehouse P.L.P Limited | Manufacturing | 2014 |
| Whitelaw Agriculture Ltd | Agriculture & fishing | 2024 |
| Whizzlets Property Solutions Limited | Real estate | 2010 |
| WHY NOT Test Equipment Limited | Wholesale & retail | 2009 |
| Wifionboard Limited | Information & communication | 2013 |
| Wild Park Leisure Ltd | Arts & recreation | 2003 |
| Willow Court (Ashbourne) RTM Company Limited | Households | 2012 |
| Windby Ltd | Wholesale & retail | 2020 |
| Windby Travel Ltd | Administrative & support | 2023 |
| Windmill Extrusions Limited | Manufacturing | 2000 |
| Windmill Stores (Retail) Limited | Wholesale & retail | 2007 |
| Winmore Engineering Limited | Professional & scientific | 2016 |
| Wood Farm (Boylestone) Management Company Ltd | Households | 2021 |
| Woodhall Radbourne Ltd | Agriculture & fishing | 2025 |
| Workshop 208 Limited | Information & communication | 2014 |
| Worldwide Ultimate Trading Ltd | Administrative & support | 2014 |
| Worthington Building Contractors Ltd | Construction | 2012 |
| Wright & Holmes Limited | Construction | 2008 |
| Wyaston Gardens Limited | Households | 1992 |
| WYE Associates Ltd | Financial services | 2006 |
| X.R.O. Chemical Services Limited | Manufacturing | 1991 |
| Y99 Ltd | Real estate | 2017 |
| Yates Plumbing & Heating Works Ltd | Construction | 2026 |
| Yeaveley Estate Limited | Arts & recreation | 1995 |
| Yellow Projects Ltd | Professional & scientific | 2024 |
| Yeomans Access ARB Ltd | Agriculture & fishing | 2026 |
| YEW Tree Records (Derbyshire) Ltd | Wholesale & retail | 2024 |
| York Place (Derby) Management Company Limited | Real estate | 2006 |
| Young Ideas Group Limited | Professional & scientific | 2007 |
| Young Ideas Limited | Wholesale & retail | 1983 |
| Your Own Space Ltd | Real estate | 2022 |
| Zanaria Ltd | Wholesale & retail | 2021 |
| Zenith Executive Coaching Limited | Professional & scientific | 2019 |
| Zollara Ltd | Professional & scientific | 2023 |
| Zulu's Landscaping & Powerwashing Company Ltd | Administrative & support | 2023 |