| LFB Vehicle Recovery Ltd | Water & waste | 2026 |
| Libra Business Essentials Limited | Professional & scientific | 2010 |
| LIC Coffee Ltd | Hospitality | 2020 |
| Lidds Clothing Ltd | Wholesale & retail | 2025 |
| Lievesley Transport Limited | Transport & logistics | 2004 |
| Lighthouse Restaurant (Ashbourne) Ltd | Hospitality | 2014 |
| Lime Tree Holiday Park Limited | Hospitality | 2011 |
| Limestone Valley Contracting Ltd | Construction | 2025 |
| Litmus Electronics Ltd | Professional & scientific | 2026 |
| Little Image Ltd | Manufacturing | 1995 |
| Little Moor Farm Limited | Construction | 2018 |
| Little Tiger Consultancy Ltd | Professional & scientific | 2025 |
| Little Trekkers Limited | Wholesale & retail | 2002 |
| Littlehotdogwatson Ltd | Wholesale & retail | 2015 |
| Livearth Ltd | Construction | 2009 |
| Living Heritage Event Hire Services Ltd. | Arts & recreation | 1986 |
| Livingstone Inn Ltd | Hospitality | 2013 |
| LK Movements Limited | Transport & logistics | 2025 |
| Lleiniog Management Company Ltd | Households | 2021 |
| LMB Peak Performance Ltd | Professional & scientific | 2023 |
| Loaf Consultancy Services Limited | Professional & scientific | 2017 |
| Loaf Horizons Ltd | Financial services | 2025 |
| Local Produce Ltd | Hospitality | 2024 |
| Lodge & Home Rentals LLP | | 2025 |
| Lomas Electrical Contractors Limited | Construction | 2003 |
| Longford Films Limited | Arts & recreation | 2018 |
| LOS Civils Limited | Construction | 2021 |
| Lourose Limited | Wholesale & retail | 2003 |
| Lower Stables Investments Limited | Financial services | 2020 |
| LTM Holdings Ltd | Administrative & support | 2026 |
| Lucannezz Recycling Limited | Water & waste | 2006 |
| Luis and Bell Limited | Professional & scientific | 2017 |
| Luis and Bell Surveyors Ltd | Professional & scientific | 2013 |
| Lupo Construction Ltd | Construction | 2025 |
| Lupo Project Management Ltd | Professional & scientific | 2025 |
| Lupo Property Group Ltd | Real estate | 2023 |
| LWB Engineering Ltd | Wholesale & retail | 2019 |
| Lyle-Smith Catering Ltd | Hospitality | 2015 |
| Lymer Procura Ltd | Professional & scientific | 2024 |
| Lyon Oak Village Ltd | Wholesale & retail | 2019 |
| M & H Gardening Solutions Limited | Administrative & support | 2016 |
| M & P Acquisitions Limited | Information & communication | 2016 |
| M & P Design Concepts Ltd | Professional & scientific | 2017 |
| M a Equestrian Limited | Agriculture & fishing | 2025 |
| M H Electrical & Solar Installations Ltd | Construction | 2021 |
| M H Pipework Installation Limited | Manufacturing | 2014 |
| M J R Commercials Limited | Wholesale & retail | 2009 |
| M Lees Builders Limited | Construction | 2004 |
| M P Wright Ltd | Agriculture & fishing | 2013 |
| M&J Premises Ltd | Real estate | 2020 |
| M-Mover Holdings Limited | Manufacturing | 2009 |
| M. J. S. Fabrications Limited | Real estate | 1986 |
| M. James Mobile Auto Electrical Services Limited | Wholesale & retail | 2002 |
| M2 Civils Ltd | Construction | 2015 |
| Macdconsult Limited | Administrative & support | 2005 |
| Macroblock Ltd | Professional & scientific | 2019 |
| Made in Britain Lighting Ltd | Manufacturing | 2020 |
| Main Street Consultants Limited | Administrative & support | 2014 |
| Mainbrand Limited | Real estate | 2002 |
| Manor Investments (Derbyshire) Limited | Professional & scientific | 2019 |
| Manusolve Limited | Manufacturing | 2011 |
| Mapperley SIX Flats Limited | Households | 2010 |
| Mapsedge Ltd | Hospitality | 2025 |
| Marcus Trans Ltd | Transport & logistics | 2022 |
| Mark Airey Enterprises Ltd | Transport & logistics | 2018 |
| Mark Bettany Joinery Limited | Construction | 2001 |
| Mark Hallam Plant Hire & Groundworks Limited | Construction | 2002 |
| Mark Harrison Plant Hire Limited | Construction | 2002 |
| Mark S Beresford Building Services Limited | Construction | 2014 |
| Markeaton Limited | Real estate | 2020 |
| Market Place Fish and Chips Limited | Hospitality | 2017 |
| Marks Family Butchers Limited | Wholesale & retail | 2012 |
| Marmalade Projects Limited | Hospitality | 2018 |
| Marsh Tech Automotive Ltd | Wholesale & retail | 2018 |
| Marshmail Investments Limited | Professional & scientific | 1989 |
| Marston Park Farm Limited | Agriculture & fishing | 2016 |
| Marston Residents Management Company Limited | Households | 2021 |
| Martin Hyde Limited | Arts & recreation | 2007 |
| Maskery Trading Ltd | Wholesale & retail | 2021 |
| Mason Lathe Ltd | Real estate | 2020 |
| Mastermover International Limited | Extraterritorial | 1994 |
| Mastermover Limited | Manufacturing | 2008 |
| Mastermover Service and Rentals Limited | Administrative & support | 2000 |
| Mates Mobiles Ashbourne Ltd | Manufacturing | 2022 |
| MAX Garage Door Repairs Limited | Other services | 2023 |
| Maxmarshallgolf Ltd | Education | 2023 |
| Mayfair Property Management Company (Derby) Limited | Extraterritorial | 1964 |
| MB Contracting Ltd | Transport & logistics | 2022 |
| MBN Catering Ltd | Hospitality | 2024 |
| MCS Build Solutions Ltd | Construction | 2025 |
| MDL (Derby) Ltd | Construction | 2012 |
| Meadow Signs Limited | Professional & scientific | 2020 |
| Medi-Kelsey Limited | Professional & scientific | 2005 |
| Meerbrook Construction Services Ltd | Construction | 2011 |
| Merci Healthcare & Solutions Ltd | Health & social care | 2022 |
| Mercia Scaffolding (Midlands) Ltd | Construction | 2024 |
| METHOD66 Ltd | Administrative & support | 2017 |
| Mewood Ltd | Manufacturing | 2024 |
| Meynell Langley Gardens Limited | Wholesale & retail | 2016 |
| MGM GAS Services Limited | Construction | 2019 |