| Elby Sings Ltd | Arts & recreation | 2026 |
| Elegant Flooring Limited | Construction | 2024 |
| Elevate Productions Ltd | Arts & recreation | 2024 |
| Elevate Referral Marketing Ltd | Other services | 2025 |
| Elevation 11 Lifts Ltd | Construction | 2026 |
| Elias Speed Ltd | Transport & logistics | 2021 |
| Elite Emporium Ltd | Wholesale & retail | 2019 |
| Elite Imports Limited | Wholesale & retail | 1986 |
| Elizabeth Tyler Ltd | Professional & scientific | 2023 |
| Elizae Investment Properties Ltd | Real estate | 2026 |
| Elpatech Ltd | Information & communication | 2015 |
| Emasco Limited | Professional & scientific | 1998 |
| Emba Collection Ltd | Wholesale & retail | 2026 |
| Embley Estates Limited | Real estate | 2017 |
| Emcat Mortgages Ltd | Financial services | 2023 |
| Emdazogs Limited | Health & social care | 2016 |
| Emily Hill Lifestyle Limited | Professional & scientific | 2022 |
| Emily Stevens - the Tutor Ltd | Education | 2022 |
| Emily the Physio Ltd | Health & social care | 2023 |
| Emka Transport Ltd | Wholesale & retail | 2011 |
| Emma Clarkson Webb Ltd | Professional & scientific | 2015 |
| Emma Hudson Technical Consultancy Ltd | Professional & scientific | 2019 |
| Emmanuel Three Angels Ltd | Education | 2026 |
| Emmwood Sweets Ltd | Hospitality | 2025 |
| Empathylab Community Interest Company | Education | 2017 |
| Enache Limited | Transport & logistics | 2018 |
| Enchanted Attire Co Ltd | Wholesale & retail | 2023 |
| Energy Safety Trust | Extraterritorial | 2009 |
| Engineering Solutions and Supply Limited | Manufacturing | 1992 |
| Engineering Supplies Limited | Professional & scientific | 2002 |
| English and Maths Tuition for YOU Limited | Education | 2020 |
| Engsol Group Ltd | Financial services | 2024 |
| Enham Binders Limited | Extraterritorial | 2004 |
| Enhance Hair Beauty & Tanning Ltd | Other services | 2017 |
| Enhance Nails Limited | Other services | 2023 |
| Enhance New Media Ltd | Professional & scientific | 2011 |
| Enjoy Natural Foods Limited | Financial services | 2014 |
| Entrepreneur Apparel Ltd | Wholesale & retail | 2023 |
| Enville Business Services Ltd | Professional & scientific | 2014 |
| Enviroboards Ltd | Construction | 2010 |
| Envy Bathrooms (LP) Ltd | Manufacturing | 2024 |
| Envy Bathrooms Ltd | Manufacturing | 2020 |
| EPF Consultancy Ltd | Real estate | 2006 |
| Epic Andover CIC | Health & social care | 2024 |
| Equicare Veterinary Services Ltd | Professional & scientific | 2020 |
| Equilibrium RNR Ltd | Professional & scientific | 2026 |
| Equity Dynamics Corporate Communications Limited | Professional & scientific | 2023 |
| ERA Group Holdings Limited | Financial services | 2021 |
| Ertransporter Ltd | Transport & logistics | 2022 |
| Es.Ka Service Ltd | Transport & logistics | 2015 |
| Eseidr Limited | Professional & scientific | 2019 |
| Esperanza Property Limited | Real estate | 2021 |
| Esperienza Learning Ltd | Information & communication | 2011 |
| Esseborne Development Limited | Construction | 2025 |
| Esseborne Estate Management Ltd | Households | 2025 |
| Esseborne Manor Hotel Limited | Hospitality | 1995 |
| Essential Fire Ltd | Construction | 2017 |
| Esta Trans Ltd | Transport & logistics | 2019 |
| Etest Systems Ltd | Information & communication | 2002 |
| Eugentrans Ltd | Transport & logistics | 2011 |
| Euronics Limited | Wholesale & retail | 1995 |
| Evees Properties Ltd | Real estate | 2024 |
| Evees Stays Ltd | Hospitality | 2026 |
| Eventsertainment Limited | Hospitality | 2020 |
| Everard Global Limited | Professional & scientific | 2020 |
| Everclean (GBR) Ltd | Administrative & support | 2025 |
| Every Freedom Ltd | Extraterritorial | 2024 |
| Everyday Nest Ltd | Wholesale & retail | 2025 |
| EVO Design and Build Ltd | Construction | 2023 |
| Evolution Agility Ltd | Other services | 2024 |
| Evolution Measurement Group Limited | Financial services | 2021 |
| Evolution Measurement Limited | Professional & scientific | 2016 |
| Evolve Websites Limited | Administrative & support | 2007 |
| Evrfit Limited | Education | 2025 |
| Exalter IT Ltd | Extraterritorial | 2015 |
| Excel Windows Limited | Construction | 2025 |
| Exotank Ltd | Manufacturing | 2021 |
| Exotic VET Support Ltd | Professional & scientific | 2019 |
| Express Strip OUT & Clearance Ltd | Water & waste | 2026 |
| Extend Managed Resource Solutions Limited | Administrative & support | 2018 |
| Exterior Maintenance Solutions UK Ltd | Administrative & support | 2026 |
| Extreme Soft Clothing Ltd | Wholesale & retail | 2024 |
| Exzibit Design Limited | Administrative & support | 2019 |
| EY18 Limited | Hospitality | 2017 |
| Ezra's Catering Services Limited | Hospitality | 2023 |
| Ezras Diner Limited | Hospitality | 2022 |
| F & D Market Ltd | Wholesale & retail | 2018 |
| F&G Management Ltd | Information & communication | 2025 |
| Fabricating Beams Contracts Trading AS FBC Ltd | Construction | 2024 |
| Fairah Limited | Real estate | 2018 |
| Fairways Cars Ltd | Transport & logistics | 2021 |
| Fairwood Owners Ltd | Households | 2005 |
| Falca Productions Ltd | Information & communication | 2026 |
| Falcon Cars & Minibuses Ltd | Transport & logistics | 2020 |
| Falk Group Limited | Professional & scientific | 2017 |
| Falsto Ltd | Real estate | 2005 |
| FAM Care Ltd | Administrative & support | 2020 |
| Family Harmony Ltd | Professional & scientific | 2024 |
| Faraday Building Contractors Ltd | Construction | 2021 |
| Faregos Home Education Group (Exams & Tuition) CIC | Education | 2016 |