| A Forward Step Ltd | Other services | 2019 |
| A P M Hydraulics Limited | Professional & scientific | 2015 |
| ABC Fleet Safe Training Limited | Transport & logistics | 2015 |
| Absolute Powder Coatings Limited | Manufacturing | 2006 |
| Acacia Property Holdings Limited | Real estate | 2018 |
| Activepure Air UK Ltd | Energy | 2022 |
| Ajar Property Ltd | Construction | 2015 |
| Alacrity Project Consultants Limited | Professional & scientific | 2017 |
| Alchemy Fireworks Limited | Arts & recreation | 2007 |
| Ampthill & District Community Rugby Club Limited | Arts & recreation | 1959 |
| Ampthill Electronic Manufacturing Services Limited | Manufacturing | 2009 |
| Ampthill Fireplaces Limited | Wholesale & retail | 2012 |
| Ampthill Glass Centre Limited | Construction | 1986 |
| Ampthill Masonic Centre Limited | Administrative & support | 1973 |
| Ampthill Metal Company Limited | Water & waste | 1979 |
| Ampthill Town FC Holdings Limited | Financial services | 2025 |
| Ampthill Town Football Club Limited | Arts & recreation | 2025 |
| Anne Rogers Soft Furnishings Limited | Other services | 2014 |
| Apex Computing Limited | Other services | 2018 |
| Arcadia Horticultural Services Limited | Administrative & support | 2018 |
| Arena Balti House Ltd | Hospitality | 2020 |
| ARM Plumbing & Heating Limited | Construction | 2011 |
| Ashburnham Road (Ampthill) Limited | Extraterritorial | 2010 |
| Atolas LLP | | 2023 |
| Aya-Uk Ltd | Hospitality | 2025 |
| B.W. Estates Limited | Real estate | 1986 |
| Bare Necessity Solutions Limited | Wholesale & retail | 2008 |
| Bare Necessity Solutions Properties Ltd | Real estate | 2020 |
| Barford Consultants Limited | Administrative & support | 2014 |
| Bedford Body Shop Holdings Limited | Professional & scientific | 2020 |
| Beetrootandblack Limited | Professional & scientific | 2023 |
| Bespoke Product Solutions Ltd | Manufacturing | 2023 |
| BI Aereboe Consulting Ltd | Administrative & support | 2024 |
| Bidfix Limited | Professional & scientific | 2018 |
| Bisumibi Limited | Other services | 2018 |
| Block 4 Porters Wood Management Company Limited | Real estate | 2005 |
| Block 5 Porters Wood Management Company Limited | Real estate | 2005 |
| BMC Sport Limited | Other services | 2025 |
| Bojie Holdings Ltd | Other services | 1996 |
| Bota Takeaway Ltd | Other services | 2022 |
| Bounce EM Ltd | Arts & recreation | 2023 |
| BR Property Hub Limited | Real estate | 2018 |
| Braywood Associates Limited | Professional & scientific | 2013 |
| Bridgnorth Consulting Limited | Professional & scientific | 2014 |
| Brit Media Company Limited | Professional & scientific | 2019 |
| Brit Media Company Pro Limited | Other services | 2024 |
| BSV Ltd | Professional & scientific | 2016 |
| BSV UK Holdings Limited | Other services | 2017 |
| C.S. Funding Limited | Financial services | 1993 |
| Caliber Financial Associates Limited | Financial services | 2001 |
| Caliber Financial Management Limited | Professional & scientific | 2007 |
| Carpenter Enterprises Limited | Financial services | 2021 |
| Carter Sullivan Learning Ltd | Education | 2017 |
| Carter Sullivan Limited | Administrative & support | 2003 |
| Case Interiors Limited | Manufacturing | 2017 |
| Cawe's Consulting Ltd | Professional & scientific | 2021 |
| Change Leadership (UK) Limited | Professional & scientific | 2005 |
| Charlotte’S Nails Lashes & Beauty Limited | Other services | 2019 |
| CKB R&D Ltd | Real estate | 2024 |
| Claribes Limited | Wholesale & retail | 1993 |
| Clarion Roadside Limited | Construction | 2005 |
| Cloud 9 Property Group Limited | Financial services | 2025 |
| Cobbitts Management Limited | Real estate | 2002 |
| Content Craving Studio Ltd | Professional & scientific | 2025 |
| Contented Homes Limited | Real estate | 2021 |
| Contract Property Interiors Ltd | Other services | 2018 |
| Cubs Business Consultancy Services Ltd | Professional & scientific | 2025 |
| Curated by Design Limited | Other services | 2025 |
| CWA BIM Limited | Other services | 2016 |
| D & H Projects Limited | Health & social care | 2008 |
| D B C Motorcycles Limited | Wholesale & retail | 2003 |
| D S Harris Leisure Limited | Hospitality | 2024 |
| Daniel James Holding Limited | Financial services | 2017 |
| David Tinker & Associates Limited | Professional & scientific | 2005 |
| Deeptech Addon Investors LLP | | 2021 |
| Deeptech Early Investors LLP | | 2021 |
| Delightfully Dressage Limited | Arts & recreation | 2026 |
| Denise Patricia Davis Ltd | Extraterritorial | 2023 |
| Dewesoft UK Ltd | Wholesale & retail | 1997 |
| DGK Consulting Limited | Information & communication | 2011 |
| Diane Butterworth Ltd | Professional & scientific | 2015 |
| Digivoxx Ltd | Administrative & support | 2016 |
| Door Access Limited | Construction | 2008 |
| DVA Consulting Ltd | Other services | 2022 |
| Dvekaria Limited | Real estate | 2016 |
| Easton Aviation Limited | Transport & logistics | 2021 |
| Ecom Products Ltd | Wholesale & retail | 2023 |
| Edison and Tilley Property Limited | Real estate | 2021 |
| Edward Consulting Services Limited | Construction | 2024 |
| Efil Limited | Professional & scientific | 2012 |
| Eisbears Limited | Other services | 2021 |
| Ekkh Limited | Other services | 2017 |
| Ella & White Ltd. | Construction | 1995 |
| Emerson Property Hub Ltd | Real estate | 2021 |
| Emerson White Limited | Information & communication | 2006 |
| Ergodinamica UK Limited | Other services | 2024 |
| ESK Hub Limited | Real estate | 2020 |
| ETH Mechanical Limited | Other services | 2017 |
| Eton Property Hub Ltd | Real estate | 2020 |
| EVO Automotive Solutions Limited | Wholesale & retail | 1999 |