| E-HUM4N Ltd | Administrative & support | 2025 |
| E-Human Ltd | Administrative & support | 2018 |
| E-Teck Limited | Wholesale & retail | 1997 |
| Ealing (T) Hairdressing Limited | Other services | 2016 |
| EAR Trumpet Creative LLP | | 2010 |
| Early Years Business Solutions Ltd | Education | 2023 |
| Eashing Lane Investments Limited | Financial services | 1955 |
| Eastbourne Cadrive Ltd | Administrative & support | 2024 |
| Easter Hill Limited | Professional & scientific | 2008 |
| Easthants Motors Ltd | Administrative & support | 2022 |
| Ebbo Civils Limited | Construction | 2016 |
| ECG Property Investments Limited | Real estate | 2020 |
| Eclipse Building Contracts Limited | Construction | 2005 |
| ECS Plumbing & Heating Ltd | Construction | 2014 |
| ECS Pumps Ltd | Water & waste | 2020 |
| ED Davey Mortgage Services Limited | Financial services | 2016 |
| ED Edits Ltd | Information & communication | 2026 |
| EDB Hairdressing Limited | Other services | 2012 |
| EDC CF Ltd | Arts & recreation | 2025 |
| EDC CF Wessex Limited | Arts & recreation | 2023 |
| EDC Performance Ltd | Arts & recreation | 2026 |
| Eden Blooms Floral Design Ltd | Wholesale & retail | 2021 |
| Eden Equine Hampshire Ltd | Agriculture & fishing | 2024 |
| Eden Escapes Body and Soul Ltd | Wholesale & retail | 2024 |
| Eden Maya Building and Landscaping Services Ltd | Construction | 2023 |
| Edge Sports & Activities Limited | Real estate | 2015 |
| Edible Hugs Limited | Manufacturing | 2020 |
| Edifice Capital Holdings | Financial services | 2024 |
| Edifice Capital Investments | Financial services | 2024 |
| Edinburgh (T) Hairdressing Limited | Other services | 2014 |
| Edradynate Limited | Real estate | 1939 |
| EDT Ventures Limited | Financial services | 2025 |
| EF Property Ltd | Real estate | 2017 |
| EG2HOME Limited | Wholesale & retail | 2018 |
| Egbert Mews Estate Company Ltd | Households | 2021 |
| Egbert Mews Management Company Ltd | Households | 2020 |
| Egginton Properties Ltd | Real estate | 2021 |
| Egham & Hythe Funeral Directors Limited | Other services | 1998 |
| EHS Equine Veterinary Medicine Limited | Professional & scientific | 2024 |
| EHS Property Limited | Real estate | 2017 |
| EJO (Elizabeth Johnson Organisation) Ltd | Administrative & support | 2011 |
| Elecnet Limited | Wholesale & retail | 2012 |
| Electrical and Pump Services Ltd | Water & waste | 2011 |
| Electrocode Limited | Information & communication | 2022 |
| Electroluminate Limited | Manufacturing | 2007 |
| Elitech International Limited | Administrative & support | 2016 |
| Elixir Aviation Services Limited | Transport & logistics | 2009 |
| Elizabeth Johnson Organisation LLP | | 2008 |
| Elizabeth Magpantay Limited | Health & social care | 2014 |
| ELK Capital Investments (UK) Limited | Financial services | 2014 |
| ELK Logistics Limited | Wholesale & retail | 2024 |
| Elkolet Humanitarian Developers Limited | Education | 2015 |
| Elkolet Ministries (UK) Limited | Education | 2014 |
| Ellis & Sons Amalgamated Investments Limited | Real estate | 1930 |
| Ellis & Sons Amalgamated Properties Limited | Real estate | 1939 |
| Ellis and Sons Third Amalgamated Properties Limited | Financial services | 1939 |
| Ellis Campbell Coxbridge Limited | Real estate | 2002 |
| Ellis Campbell Developments Limited | Real estate | 1934 |
| Ellis Campbell Group Ltd | Financial services | 2020 |
| Ellis Campbell Holdings Limited | Financial services | 1940 |
| Ellis Campbell Trading Ltd | Financial services | 2017 |
| Elliver Asset Management Ltd | Financial services | 2024 |
| ELM Solutions Limited | Manufacturing | 2020 |
| Elstead Group Holdings Ltd | Financial services | 2021 |
| Elstead Group Limited | Administrative & support | 1999 |
| Elstead Lighting Limited | Manufacturing | 1969 |
| Embers and Ice Newbury Ltd | Hospitality | 2025 |
| Emby Engineering Limited | Extraterritorial | 2021 |
| Emily Trewhella-Clark Limited | Real estate | 2025 |
| Emmerson Property Investments Ltd | Real estate | 2021 |
| Emoby Limited | Information & communication | 2025 |
| Empress Laboratories Ltd | Professional & scientific | 2020 |
| Empshott LLP | | 2024 |
| Endeavour Place Management Company Limited | Households | 2005 |
| Energique Health Club & Spa Ltd | Administrative & support | 2011 |
| Energique Holdings Ltd | Financial services | 2025 |
| Energy Electrical Distributors Limited | Wholesale & retail | 2012 |
| Enfranchisement Capital Holdings Ltd | Administrative & support | 2003 |
| Engelmann & Buckham Ltd | Wholesale & retail | 2001 |
| English Hampshire Lavender Limited | Wholesale & retail | 1999 |
| Enitharmon Press Limited | Extraterritorial | 2010 |
| Enquiry Into Thought Limited | Professional & scientific | 2013 |
| Epsom (T) Hairdressing Limited | Other services | 2010 |
| Equalbrief Limited | Manufacturing | 1988 |
| Equinox Contemporary Jewellery Limited | Wholesale & retail | 2011 |
| Erganit Ltd | Information & communication | 2023 |
| Esperanza Consultants Ltd | Administrative & support | 2016 |
| Essence Hair Limited | Other services | 1999 |
| Essensuals Group Limited | Other services | 1997 |
| Estream Communications UK Limited | Information & communication | 2014 |
| Ethicheck Limited | Wholesale & retail | 2014 |
| Eureka PR and Communications Ltd | Administrative & support | 2005 |
| Eurofire Protection Limited | Professional & scientific | 2008 |
| Eurotech Graphics Limited | Professional & scientific | 1998 |
| EV Home Chargers Limited | Construction | 2024 |
| Everest UK Finco Limited | Financial services | 2018 |
| Everest UK Hedgeco Limited | Financial services | 2018 |
| Everwood Furniture Studio Ltd | Manufacturing | 2019 |
| Evolve Mortgage Advice Ltd | Financial services | 2019 |
| Evolvexconsultancy Limited | Professional & scientific | 2026 |