| A Hutchinson Services Ltd | Transport & logistics | 2025 |
| Access and Inspection Solutions Ltd | Construction | 2021 |
| ALL Things Ecommerce Ltd | Wholesale & retail | 2013 |
| Alston Moor Fitness Club Limited | Arts & recreation | 2003 |
| Alston Moor Partnership Limited | Other services | 2004 |
| Alston Moor Taxis Ltd | Transport & logistics | 2026 |
| Alston MOT Centre Ltd | Wholesale & retail | 2025 |
| Alston Pharmacy Limited | Health & social care | 2010 |
| Alston Pre-School | Education | 2013 |
| Alston Taxis Ltd | Transport & logistics | 2026 |
| Alston Wholefoods Shop LLP | | 2009 |
| Appgov Ltd | Information & communication | 2012 |
| Architectural Design and Planning Limited | Professional & scientific | 2005 |
| Aroma Essential Oils Ltd | Wholesale & retail | 2023 |
| Ayle Property Company Limited | Extraterritorial | 1997 |
| Berry Management Consultants Limited | Professional & scientific | 2010 |
| Brassmonkey Technical Services Ltd | Professional & scientific | 2014 |
| Broken Illusion Pictures Ltd | Information & communication | 2022 |
| C Wood Services Limited | Agriculture & fishing | 2022 |
| Cimec Limited | Professional & scientific | 2016 |
| Concise Consulting Limited | Information & communication | 1997 |
| CW Business Group Limited | Wholesale & retail | 2009 |
| Cybermoor Services Ltd | Information & communication | 2003 |
| DAC Building Maintenance Ltd | Construction | 2010 |
| DAC National Group Limited | Construction | 2013 |
| Darkwood Forge Ltd | Manufacturing | 2024 |
| DJ Contract Services Limited | Information & communication | 2009 |
| Eco Natural Ltd | Wholesale & retail | 2022 |
| Ecofranking Ltd | Extraterritorial | 2009 |
| Feco Pharmaceuticals Ltd | Agriculture & fishing | 2024 |
| Fell Rebel Fitness Limited | Arts & recreation | 2023 |
| Fellside Forestry Ltd | Agriculture & fishing | 2021 |
| Gary G Services Limited | Construction | 2018 |
| Gecw Consulting Limited | Professional & scientific | 2026 |
| George & Dragon Inn Ltd | Hospitality | 2019 |
| Gilderdale Forest School Limited | Education | 2020 |
| Glasstap Limited | Information & communication | 1999 |
| Green Grout Limited | Construction | 2026 |
| Heljay Limited | Information & communication | 2006 |
| HI Mill CIC | Construction | 2023 |
| High Galligill Farm Ltd | Agriculture & fishing | 2020 |
| Hudson Associates (Surrey) Limited | Financial services | 2003 |
| Hymers Construction Ltd | Construction | 2018 |
| J & M Building Limited | Construction | 2018 |
| J E Land and Developments Limited | Real estate | 2017 |
| J.H. Henderson and Sons (Haulage) Limited | Transport & logistics | 1977 |
| J.H. Henderson and Sons Limited | Wholesale & retail | 1955 |
| Jads Enterprises Limited | Real estate | 2006 |
| Jads Estate Planning Ltd | Professional & scientific | 2026 |
| Jads Financial Services Ltd | Financial services | 2022 |
| Jads Lettings Limited | Real estate | 2019 |
| JET Physics Limited | Professional & scientific | 2009 |
| JG Heating Solutions Limited | Construction | 2013 |
| Ludlow Property Holdings Ltd | Real estate | 2026 |
| Ludlow Property Solutions Ltd | Construction | 2021 |
| MAD Hatters Hardware Limited | Wholesale & retail | 2022 |
| Mahalo RAE Ltd | Professional & scientific | 2022 |
| MBN Puretech Ltd | Wholesale & retail | 2016 |
| MFN Technology Limited | Manufacturing | 2005 |
| Moorlands Candles Limited | Manufacturing | 2018 |
| Moorwell Health Ltd | Health & social care | 2024 |
| Nent Hall Hotel Limited | Hospitality | 2025 |
| Nenthead Community Works C.I.C. | Other services | 2011 |
| Old Stone Limited | Professional & scientific | 2005 |
| ONE23MXCOMPETITIONS Ltd | Other services | 2023 |
| Overwater Management Company Limited | Households | 2026 |
| Paymán Holdings 7 Ltd | Hospitality | 2020 |
| Pennine Performance Ltd | Wholesale & retail | 2020 |
| Pennine Ways Holiday Cottages Ltd | Hospitality | 2022 |
| Pinnacle Law Limited | Professional & scientific | 2015 |
| Quebec Sierra Limited | Professional & scientific | 2018 |
| Raise Park Developments Limited | Other services | 2011 |
| Red FOX Consulting Limited | Professional & scientific | 2013 |
| Renwick Fencing Limited | Agriculture & fishing | 2016 |
| Renwick Property Services Limited | Real estate | 2024 |
| Richard Renwick Builders Limited | Construction | 2015 |
| Seljahol Limited | Agriculture & fishing | 2010 |
| SGW Wealth Management Ltd | Financial services | 2020 |
| Stamford, Hart and Gold Ltd | Administrative & support | 2023 |
| Tautenay Limited | Professional & scientific | 2026 |
| THE Chat Room CIC | Health & social care | 2022 |
| THE Creators Cortex Ltd | Real estate | 2022 |
| THE Cumberland Hotel LLP | | 2007 |
| THE Moors Inn Ltd | Hospitality | 2025 |
| Totalpost Mailing Ltd | Manufacturing | 2007 |
| Tower Web Design Limited | Information & communication | 2013 |
| Trainers' Library Limited | Extraterritorial | 2003 |
| Urban Horse Ltd | Extraterritorial | 2009 |
| Walter's Alston Ltd | Real estate | 2025 |
| Wanwood Holidays Limited | Hospitality | 2023 |
| West Cocklake Farm Ltd | Agriculture & fishing | 2020 |
| Westbrook Engineering Ltd | Professional & scientific | 2022 |
| Wright-Brodie Homes Ltd | Households | 2025 |