| THE Paddock (Alresford) Management Company Ltd | Households | 2017 |
| THE Retro Cleaning Company Limited | Administrative & support | 2011 |
| THE South Downs Olive OIL Company Limited | Agriculture & fishing | 2021 |
| THE Travelling Naturalist Limited | Extraterritorial | 2016 |
| THE Waney Edge Ltd | Manufacturing | 2011 |
| THE Winchester Film Society | Arts & recreation | 1994 |
| Thomas Miller Creative Ltd | Professional & scientific | 2014 |
| Three Hands Ltd | Other services | 2002 |
| Three Points Advisory Ltd | Professional & scientific | 2012 |
| Tichborne Estate Limited Partnership | | 2020 |
| Tjvn Limited | Professional & scientific | 2025 |
| Torf House Consulting Limited | Professional & scientific | 2022 |
| Total Food Solutions Sales Ltd | Wholesale & retail | 2024 |
| Town Arms Limited | Hospitality | 2014 |
| Townend Stables LLP | | 2025 |
| Tranquilo | Financial services | 2018 |
| Travelling Clays Limited | Arts & recreation | 2007 |
| Trio Link Healthcare Ltd | Administrative & support | 2025 |
| Truenorth Agile Consulting Ltd | Information & communication | 2025 |
| TTS Star Ltd | Wholesale & retail | 2016 |
| Tumra Limited | Information & communication | 2012 |
| Turnure LLP | | 2025 |
| Two Scoops Consulting Ltd | Professional & scientific | 2018 |
| Twynamite Productions Limited | Arts & recreation | 2013 |
| Tyler Medcalf Ltd | Professional & scientific | 2012 |
| UHF (Woodlands) Ltd. | Households | 1997 |
| Ukonga Ltd | Financial services | 2023 |
| UMC Brands Limited | Wholesale & retail | 2018 |
| Upper Itchen Restoration Community Interest Company | Professional & scientific | 2024 |
| Upper Wield Farm Residents Management Company Limited | Households | 1988 |
| Uptime Limited | Wholesale & retail | 1986 |
| Urbanhold Partners Ltd | Real estate | 2025 |
| Ursus Ventures Limited | Professional & scientific | 2004 |
| VB Functions UK Ltd | Hospitality | 2024 |
| Velvet Shark Productions Limited | Information & communication | 2003 |
| Vereenigde Oostindische Compagnie Ltd | Professional & scientific | 2024 |
| Vertic Arts Limited | Wholesale & retail | 2014 |
| VIDE01 Ltd | Information & communication | 2022 |
| Vision Electrical Contracting Limited | Construction | 2019 |
| Vita Multi Academy Trust | Education | 2011 |
| VMG Yacht Management Ltd | Professional & scientific | 2013 |
| W Appleton Builder Ltd | Construction | 2008 |
| Wadebridge (34007) Locomotive Limited | Transport & logistics | 1982 |
| Wakefield Partners Limited | Financial services | 2013 |
| Wakeham Farms Limited | Agriculture & fishing | 2023 |
| Waldron Trading Limited | Wholesale & retail | 2013 |
| Walks Worldwide Limited | Administrative & support | 2010 |
| Wallers ASH Consulting Limited | Administrative & support | 2007 |
| Walters PM Ltd | Professional & scientific | 2026 |
| Warwick Court (Alresford) Management Company Limited | Hospitality | 2009 |
| Watercress Holidays Ltd | Hospitality | 2022 |
| Waterlily Luxe Ltd | Hospitality | 2025 |
| Wave Rise Ltd | Professional & scientific | 2025 |
| Wayside Technologies Limited | Professional & scientific | 2000 |
| WE Have Heads Ltd | Arts & recreation | 2026 |
| WEF Bramdean Company Limited | Real estate | 2022 |
| Wellbeing Hub Limited | Health & social care | 2016 |
| Wellington Partners Ltd | Professional & scientific | 2020 |
| Wessex Medical Care Limited | Health & social care | 2021 |
| Wessex Pharmacies Limited | Wholesale & retail | 1942 |
| Westbourne Freeholder Ltd | Households | 2026 |
| White Worxs Ltd | Construction | 2012 |
| Whittome Developments Limited | Extraterritorial | 2023 |
| Whittome Properties Limited | Extraterritorial | 2023 |
| Wield Wines Limited | Financial services | 2010 |
| Wild Ambitions Limited | Extraterritorial | 2001 |
| Wilde and Well Company Ltd | Professional & scientific | 2026 |
| Wilder Magazine Ltd | Information & communication | 2021 |
| Wildfair Ltd | Extraterritorial | 2021 |
| Wildlife Positive Travel Ltd | Professional & scientific | 2023 |
| Wildlife Worldwide Limited | Extraterritorial | 2010 |
| Wildones Kids Limited | Wholesale & retail | 2020 |
| Williams of Bordon Limited | Water & waste | 1995 |
| Winchester Awnings Limited | Manufacturing | 2021 |
| Winchester Diocesan Board of Finance(The) | Other services | 1915 |
| Winchester House Management Limited | Households | 2022 |
| Winchesterhr Limited | Professional & scientific | 2017 |
| Winnie Consultancy LLP | | 2025 |
| Wintil Services Limited | Transport & logistics | 2024 |
| WJS Hardacre Limited | Professional & scientific | 2015 |
| WL Enterprises Ltd | Administrative & support | 2026 |
| Wonwell Limited | Real estate | 1999 |
| Woods Mortgages Ltd | Financial services | 2021 |
| Woolpack Operating Co Limited | Hospitality | 2012 |
| Wordsalad Publications Limited | Information & communication | 2004 |
| X-Point Solutions Ltd | Information & communication | 2007 |
| Xena Life Ltd | Health & social care | 2023 |
| Xkvate Ltd | Administrative & support | 2002 |
| Yasmin Ingham Eventing Limited | Arts & recreation | 2024 |
| Yettie Ltd | Manufacturing | 2019 |
| Your Home Care Limited | Health & social care | 2026 |
| Zantar Limited | Administrative & support | 2005 |
| Zetooni Ltd | Wholesale & retail | 2026 |