| Scottish Autism | Education | 1982 |
| SCR Plant Hire Ltd | Construction | 2015 |
| Screwz Limited | Wholesale & retail | 2012 |
| Secret Dojo Limited | Manufacturing | 2010 |
| Service Print Solutions Ltd | Manufacturing | 2023 |
| Seven Events Scotland Limited | Hospitality | 2024 |
| SFB Scotland Ltd | Manufacturing | 2024 |
| SG Pro Cleaning Limited | Administrative & support | 2008 |
| Shahmeer Nextgen Limited | Wholesale & retail | 2025 |
| Shami.Nn Limited | Wholesale & retail | 2022 |
| Sheepdog Coffee Ltd | Manufacturing | 2025 |
| Shelley’S Munchies Ltd | Hospitality | 2026 |
| Shire Cleaning Ltd | Administrative & support | 2023 |
| Shire Construction (Scotland) Limited | Construction | 2017 |
| Simsense Golf Ltd | Wholesale & retail | 2026 |
| SJ Coating Consultancy Limited | Professional & scientific | 2019 |
| SJH Johnson Construction Ltd | Construction | 2026 |
| Sjjc Electrical Ltd | Construction | 2023 |
| Skin Clinic 360 Limited | Health & social care | 2019 |
| Skytech Solar Ltd | Construction | 2022 |
| Skyview Surveys Ltd | Information & communication | 2024 |
| Slanjevents Ltd | Administrative & support | 2021 |
| SM Scot Ltd | Wholesale & retail | 2024 |
| Smac Studios Ltd | Arts & recreation | 2024 |
| Smart POS Ltd | Information & communication | 2026 |
| SMC Retail Ltd | Wholesale & retail | 2026 |
| Smextra Limited | Professional & scientific | 2021 |
| Smith & Son’S Roofing Services Ltd | Construction | 2026 |
| Smith Investment Property Ltd | Real estate | 2021 |
| Smiths Short Bark and Sides Ltd | Other services | 2019 |
| Snax Alloa Ltd | Wholesale & retail | 2020 |
| Solar Service Team Ltd | Construction | 2026 |
| Sorcha Ltd | Information & communication | 2023 |
| Spectrum Consulting (International) Ltd | Professional & scientific | 2020 |
| Spices Alloa (Scott) Ltd | Hospitality | 2024 |
| Spirit Tile and Stone Limited | Construction | 2021 |
| Sports Markings Scotland Limited | Arts & recreation | 2023 |
| Springking Marketing Ltd | Professional & scientific | 2024 |
| Spruce Landscapes Ltd | Administrative & support | 2026 |
| Squax Ltd | Information & communication | 2022 |
| SS Desserts Limited | Hospitality | 2021 |
| SSJ Property Ltd | Real estate | 2021 |
| SSJ Retail Ltd | Wholesale & retail | 2017 |
| SSJ Services Scotland Ltd | Administrative & support | 2021 |
| ST David's (Bathgate) Limited | Real estate | 2009 |
| Stagg and Woolfe Ltd | Construction | 2025 |
| Standguide Scotland Ltd | Administrative & support | 2024 |
| Stepping Bricks Training Ltd | Education | 2023 |
| Sterling Roofing Limited | Construction | 2025 |
| Stewart Accounting Services Limited | Professional & scientific | 2009 |
| Stewart Industrial Limited | Manufacturing | 2024 |
| Stirling Leisure Management Ltd | Administrative & support | 2023 |
| Stirling Storage Solutions Ltd | Transport & logistics | 2020 |
| Stirlingshire Vending (Scotland) Limited | Administrative & support | 2011 |
| Stuart & SON Haulage Ltd | Transport & logistics | 2026 |
| Studio Erda Limited | Professional & scientific | 2025 |
| Studio TGT Ltd | Manufacturing | 2026 |
| Style Hair & Beauty Ltd | Other services | 2019 |
| Sublime Beauty Aesthetics Ltd | Other services | 2019 |
| Sun Tron Ltd | Other services | 2022 |
| Surface Investments Limited | Administrative & support | 2013 |
| Sutcliffe Jones Pharmaceutical Ltd | Wholesale & retail | 2023 |
| SWD UK Engineering Ltd | Manufacturing | 2024 |
| Sweet HEAVEN1 Limited | Hospitality | 2025 |
| SZ Express Ltd | Hospitality | 2019 |
| SZH Residential Limited | Real estate | 2026 |
| T D Super Stores Ltd | Wholesale & retail | 2025 |
| Tablet Academy (Scotland) Limited | Information & communication | 2013 |
| Taxi Management Limited | Transport & logistics | 2010 |
| Taxtax Accountants Ltd | Professional & scientific | 2021 |
| Taylor-Duncan Exterior Cleaning Ltd | Administrative & support | 2026 |
| Taylor-Duncan Investments Ltd | Real estate | 2026 |
| TBS 25 Ltd | Hospitality | 2025 |
| Tejal Retail Ltd | Wholesale & retail | 2025 |
| THE BEE Seen Company Ltd | Information & communication | 2024 |
| THE Boiler and Bathroom Centre Ltd | Wholesale & retail | 2025 |
| THE Business Development Accountant Limited | Professional & scientific | 2024 |
| THE Construction Accountant Limited | Professional & scientific | 2024 |
| THE Dream Tree House Ltd | Wholesale & retail | 2019 |
| THE Emperors Cards Limited | Manufacturing | 2024 |
| THE Falcon Fish and Chip Company Ltd | Hospitality | 2022 |
| THE Fuel House Cafe Limited | Hospitality | 2019 |
| THE Hifi Gallery Ltd | Wholesale & retail | 2020 |
| THE INK Balm Ltd | Wholesale & retail | 2022 |
| THE Innovation Agency Ltd | Information & communication | 2023 |
| THE Ladybird Tea Room Limited | Hospitality | 2015 |
| THE Landlord Accountant Limited | Professional & scientific | 2024 |
| THE New Fangled Glass Company Limited | Manufacturing | 2007 |
| THE Oak Alloa Limited | Hospitality | 2016 |
| THE Oaky Limited | Wholesale & retail | 2019 |
| THE Ochil Fudge Pantry Limited | Manufacturing | 2017 |
| THE Problem MMA Limited | Education | 2025 |
| THE Problem Property's Limited | Real estate | 2025 |
| THE Problem Store Ltd | Wholesale & retail | 2026 |
| THE Proglaze Group Limited | Construction | 2025 |
| THE Proper Pioneer Ltd | Extraterritorial | 2025 |
| THE Roofing Bureau Ltd | Construction | 2025 |
| THE Ross Consultancy Limited | Professional & scientific | 2017 |
| THE Social Media Coach Ltd | Professional & scientific | 2023 |
| THE Tartan Rooster Ltd | Hospitality | 2020 |